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A novel sensitive cell-based Love Wave biosensor for marine toxin detection.
Biosens Bioelectron. 2016 Mar 15; 77:573-9.BB

Abstract

A novel HepG2 cell-based biosensor using Love Wave sensor was developed to implement the real-time and sensitive detection of a diarrheic shellfish poisoning (DSP) toxin, Okadaic acid (OA). Detachable Love Wave sensor unit and miniaturized 8-channel recording instrument were designed for the convenient experimental preparation and sensor response signal measurement. The Love Wave sensor, whose synchronous frequency is around 160 MHz, was fabricated with ST-cut quartz substrate. To establish a cell-based biosensor, HepG2 cells as sensing elements were cultured onto the Love Wave sensor surface, and the cell attachment process was recorded by this biosensor. Results showed this sensor could monitor the cell attachment process in real time and response signals were related to the initial cell seeding densities. Furthermore, cell-based Love Wave sensor was treated with OA toxin. This biosensor presented a good performance to various OA concentrations, with a wide linear detection range (10-100 μg/L). Based on the ultrasensitive acoustic wave platform, this cell-based biosensor will be a promising tool for real-time and convenient OA screening.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.

Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.

Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.

Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.

Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.

Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.

Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China. Electronic address: huning@zju.edu.cn.

Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China. Electronic address: cnpwang@zju.edu.cn.

MeSH

Biological AssayBiosensing TechniquesCell AdhesionCell SurvivalEquipment DesignEquipment Failure AnalysisHep G2 CellsHumansMarine ToxinsMicro-Electrical-Mechanical SystemsOkadaic AcidReproducibility of ResultsSensitivity and SpecificityToxicity Tests

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26476015

Citation

Zhang, Xi, et al. "A Novel Sensitive Cell-based Love Wave Biosensor for Marine Toxin Detection." Biosensors & Bioelectronics, vol. 77, 2016, pp. 573-9.

Zhang X, Fang J, Zou L, et al. A novel sensitive cell-based Love Wave biosensor for marine toxin detection. Biosens Bioelectron. 2016;77:573-9.

Zhang, X., Fang, J., Zou, L., Zou, Y., Lang, L., Gao, F., Hu, N., & Wang, P. (2016). A novel sensitive cell-based Love Wave biosensor for marine toxin detection. Biosensors & Bioelectronics, 77, 573-9. https://doi.org/10.1016/j.bios.2015.07.062

Zhang X, et al. A Novel Sensitive Cell-based Love Wave Biosensor for Marine Toxin Detection. Biosens Bioelectron. 2016 Mar 15;77:573-9. PubMed PMID: 26476015.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - A novel sensitive cell-based Love Wave biosensor for marine toxin detection. AU - Zhang,Xi, AU - Fang,Jiaru, AU - Zou,Ling, AU - Zou,Yingchang, AU - Lang,Lang, AU - Gao,Fan, AU - Hu,Ning, AU - Wang,Ping, Y1 - 2015/07/29/ PY - 2015/05/23/received PY - 2015/07/22/revised PY - 2015/07/27/accepted PY - 2015/10/18/entrez PY - 2015/10/18/pubmed PY - 2016/9/13/medline KW - Cell attachment KW - Cell-based biosensor KW - Love Wave sensor KW - Okadaic acid (OA) SP - 573 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 77 N2 - A novel HepG2 cell-based biosensor using Love Wave sensor was developed to implement the real-time and sensitive detection of a diarrheic shellfish poisoning (DSP) toxin, Okadaic acid (OA). Detachable Love Wave sensor unit and miniaturized 8-channel recording instrument were designed for the convenient experimental preparation and sensor response signal measurement. The Love Wave sensor, whose synchronous frequency is around 160 MHz, was fabricated with ST-cut quartz substrate. To establish a cell-based biosensor, HepG2 cells as sensing elements were cultured onto the Love Wave sensor surface, and the cell attachment process was recorded by this biosensor. Results showed this sensor could monitor the cell attachment process in real time and response signals were related to the initial cell seeding densities. Furthermore, cell-based Love Wave sensor was treated with OA toxin. This biosensor presented a good performance to various OA concentrations, with a wide linear detection range (10-100 μg/L). Based on the ultrasensitive acoustic wave platform, this cell-based biosensor will be a promising tool for real-time and convenient OA screening. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/26476015/A_novel_sensitive_cell_based_Love_Wave_biosensor_for_marine_toxin_detection_ DB - PRIME DP - Unbound Medicine ER -