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Interferon-γ promotes phagocytosis of Cryptococcus neoformans but not Cryptococcus gattii by murine macrophages.
J Infect Chemother. 2015 Dec; 21(12):831-6.JI

Abstract

Among invasive fungal infections, cryptococcosis caused by inhalation of Cryptococcus neoformans or Cryptococcus gattii is particularly dangerous because it can disseminate to the central nervous system and cause life-threatening meningitis or meningoencephalitis. Previous reports described significant differences in the histopathological features of C. neoformans and C. gattii infection, such as greater pathogen proliferation and a limited macrophage response in mouse lung infected by C. gattii. To elucidate the difference in pathogenicity of these two Cryptococcus species, we investigated the interaction of C. neoformans and C. gattii with murine macrophages, the first line of host defense, by confocal laser microscopy. Only thin-capsulated, and not thick-capsulated C. neoformans and C. gattii were phagocytosed by macrophages. Preactivation with interferon-γ increased the phagocytic rate of thin-capsulated C. neoformans up to two-fold, but did not promote phagocytosis of thin-capsulated C. gattii. Lipopolysaccharide preactivation or Aspergillus fumigatus conidia co-incubation had no effect on internalization of thin-capsulated C. neoformans or C. gattii by macrophages. Phagocytosis of live thin-capsulated C. neoformans, but not that of live thin-capsulated C. gattii, induced interleukin-12 release from macrophages. However, phagocytosis of heat-killed or paraformaldehyde-fixed thin-capsulated C. neoformans did not increase IL-12 release, showing that the internalization of live yeast is important for initiating the immune response during C. neoformans-macrophage interactions. Our data suggest that macrophage response to C. gattii is limited compared with that to C. neoformans and that these results may partially explain the limited immune response and the greater pathogenicity of C. gattii.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ikeda-Dantsuji Y

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan.

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan; Department of Infectious Diseases and Infection Control, Saitama Medical Center, Saitama Medical University, Saitama, Japan.

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan.

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan.

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan.

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan.

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan.

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan.

Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan. Electronic address: ym46@niid.go.jp.

MeSH

AnimalsCell LineCryptococcosisCryptococcus gattiiCryptococcus neoformansInterferon-gammaInterleukin-12LipopolysaccharidesLungMacrophagesMicePhagocytosis

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26477011

Citation

Ikeda-Dantsuji, Yurika, et al. "Interferon-γ Promotes Phagocytosis of Cryptococcus Neoformans but Not Cryptococcus Gattii By Murine Macrophages." Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy, vol. 21, no. 12, 2015, pp. 831-6.

Ikeda-Dantsuji Y, Ohno H, Tanabe K, et al. Interferon-γ promotes phagocytosis of Cryptococcus neoformans but not Cryptococcus gattii by murine macrophages. J Infect Chemother. 2015;21(12):831-6.

Ikeda-Dantsuji, Y., Ohno, H., Tanabe, K., Umeyama, T., Ueno, K., Nagi, M., Yamagoe, S., Kinjo, Y., & Miyazaki, Y. (2015). Interferon-γ promotes phagocytosis of Cryptococcus neoformans but not Cryptococcus gattii by murine macrophages. Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy, 21(12), 831-6. https://doi.org/10.1016/j.jiac.2015.08.001

Ikeda-Dantsuji Y, et al. Interferon-γ Promotes Phagocytosis of Cryptococcus Neoformans but Not Cryptococcus Gattii By Murine Macrophages. J Infect Chemother. 2015;21(12):831-6. PubMed PMID: 26477011.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Interferon-γ promotes phagocytosis of Cryptococcus neoformans but not Cryptococcus gattii by murine macrophages. AU - Ikeda-Dantsuji,Yurika, AU - Ohno,Hideaki, AU - Tanabe,Koichi, AU - Umeyama,Takashi, AU - Ueno,Keigo, AU - Nagi,Minoru, AU - Yamagoe,Satoshi, AU - Kinjo,Yuki, AU - Miyazaki,Yoshitsugu, Y1 - 2015/10/23/ PY - 2015/06/03/received PY - 2015/07/23/revised PY - 2015/08/07/accepted PY - 2015/10/19/entrez PY - 2015/10/20/pubmed PY - 2016/9/9/medline KW - Cryptococcus gattii KW - Cryptococcus neoformans KW - Interferon-γ KW - Interleukin-12 KW - Macrophage KW - Phagocytosis SP - 831 EP - 6 JF - Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy JO - J Infect Chemother VL - 21 IS - 12 N2 - Among invasive fungal infections, cryptococcosis caused by inhalation of Cryptococcus neoformans or Cryptococcus gattii is particularly dangerous because it can disseminate to the central nervous system and cause life-threatening meningitis or meningoencephalitis. Previous reports described significant differences in the histopathological features of C. neoformans and C. gattii infection, such as greater pathogen proliferation and a limited macrophage response in mouse lung infected by C. gattii. To elucidate the difference in pathogenicity of these two Cryptococcus species, we investigated the interaction of C. neoformans and C. gattii with murine macrophages, the first line of host defense, by confocal laser microscopy. Only thin-capsulated, and not thick-capsulated C. neoformans and C. gattii were phagocytosed by macrophages. Preactivation with interferon-γ increased the phagocytic rate of thin-capsulated C. neoformans up to two-fold, but did not promote phagocytosis of thin-capsulated C. gattii. Lipopolysaccharide preactivation or Aspergillus fumigatus conidia co-incubation had no effect on internalization of thin-capsulated C. neoformans or C. gattii by macrophages. Phagocytosis of live thin-capsulated C. neoformans, but not that of live thin-capsulated C. gattii, induced interleukin-12 release from macrophages. However, phagocytosis of heat-killed or paraformaldehyde-fixed thin-capsulated C. neoformans did not increase IL-12 release, showing that the internalization of live yeast is important for initiating the immune response during C. neoformans-macrophage interactions. Our data suggest that macrophage response to C. gattii is limited compared with that to C. neoformans and that these results may partially explain the limited immune response and the greater pathogenicity of C. gattii. SN - 1437-7780 UR - https://www.unboundmedicine.com/medline/citation/26477011/Interferon_γ_promotes_phagocytosis_of_Cryptococcus_neoformans_but_not_Cryptococcus_gattii_by_murine_macrophages_ DB - PRIME DP - Unbound Medicine ER -