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Tailor-Made Boronic Acid Functionalized Magnetic Nanoparticles with a Tunable Polymer Shell-Assisted for the Selective Enrichment of Glycoproteins/Glycopeptides.
ACS Appl Mater Interfaces 2015; 7(44):24576-84AA

Abstract

Biomedical sciences, and in particular biomarker research, demand efficient glycoproteins enrichment platforms. In this work, we present a facile and time-saving method to synthesize phenylboronic acid and copolymer multifunctionalized magnetic nanoparticles (NPs) using a distillation-precipitation polymerization (DPP) technique. The polymer shell is obtained through copolymerization of two monomers-affinity ligand 3-acrylaminophenylboronic acid (AAPBA) and a hydrophilic functional monomer. The resulting hydrophilic Fe3O4@P(AAPBA-co-monomer) NPs exhibit an enhanced binding capacity toward glycoproteins by an additional functional monomer complementary to the surface presentation of the target protein. The effects of monomer ratio of AAPBA to hydrophilic comonomers on the binding of glycoproteins are systematically investigated. The morphology, structure, and composition of all the synthesized microspheres are characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM). The hydrophilic Fe3O4@P(AAPBA-co-monomer) microspheres show an excellent performance in the separation of glycoproteins with high binding capacity; And strong magnetic response allows them to be easily separated from solution in the presence of an external magnetic field. Moreover, both synthetic Fe3O4@P(AAPBA) and copolymeric NPs show good adsorption to glycoproteins in physiological conditions (pH 7.4). The Fe3O4@P(AAPBA-co-monomer) NPs are successfully utilized to selectively capture and identify the low-abundance glycopeptides from the tryptic digest of horseradish peroxidase (HRP). In addition, the selective isolation and enrichment of glycoproteins from the egg white samples at physiological condition is obtained by Fe3O4@P(AAPBA-co-monomer) NPs as adsorbents.

Authors+Show Affiliations

Research Center for Analytical Sciences, College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin 300071, China. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.Research Center for Analytical Sciences, College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin 300071, China. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.Research Center for Analytical Sciences, College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin 300071, China. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.Research Center for Analytical Sciences, College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin 300071, China. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.Research Center for Analytical Sciences, College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Nankai University , Tianjin 300071, China. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China. Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116011, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26479332

Citation

Zhang, Xihao, et al. "Tailor-Made Boronic Acid Functionalized Magnetic Nanoparticles With a Tunable Polymer Shell-Assisted for the Selective Enrichment of Glycoproteins/Glycopeptides." ACS Applied Materials & Interfaces, vol. 7, no. 44, 2015, pp. 24576-84.
Zhang X, Wang J, He X, et al. Tailor-Made Boronic Acid Functionalized Magnetic Nanoparticles with a Tunable Polymer Shell-Assisted for the Selective Enrichment of Glycoproteins/Glycopeptides. ACS Appl Mater Interfaces. 2015;7(44):24576-84.
Zhang, X., Wang, J., He, X., Chen, L., & Zhang, Y. (2015). Tailor-Made Boronic Acid Functionalized Magnetic Nanoparticles with a Tunable Polymer Shell-Assisted for the Selective Enrichment of Glycoproteins/Glycopeptides. ACS Applied Materials & Interfaces, 7(44), pp. 24576-84. doi:10.1021/acsami.5b06445.
Zhang X, et al. Tailor-Made Boronic Acid Functionalized Magnetic Nanoparticles With a Tunable Polymer Shell-Assisted for the Selective Enrichment of Glycoproteins/Glycopeptides. ACS Appl Mater Interfaces. 2015 Nov 11;7(44):24576-84. PubMed PMID: 26479332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tailor-Made Boronic Acid Functionalized Magnetic Nanoparticles with a Tunable Polymer Shell-Assisted for the Selective Enrichment of Glycoproteins/Glycopeptides. AU - Zhang,Xihao, AU - Wang,Jiewen, AU - He,Xiwen, AU - Chen,Langxing, AU - Zhang,Yukui, Y1 - 2015/10/29/ PY - 2015/10/20/entrez PY - 2015/10/20/pubmed PY - 2016/10/7/medline KW - Boronate KW - Distillation−precipitation polymerization KW - Enrichment KW - Glycopeptides KW - Glycoproteins KW - Magnetic particles SP - 24576 EP - 84 JF - ACS applied materials & interfaces JO - ACS Appl Mater Interfaces VL - 7 IS - 44 N2 - Biomedical sciences, and in particular biomarker research, demand efficient glycoproteins enrichment platforms. In this work, we present a facile and time-saving method to synthesize phenylboronic acid and copolymer multifunctionalized magnetic nanoparticles (NPs) using a distillation-precipitation polymerization (DPP) technique. The polymer shell is obtained through copolymerization of two monomers-affinity ligand 3-acrylaminophenylboronic acid (AAPBA) and a hydrophilic functional monomer. The resulting hydrophilic Fe3O4@P(AAPBA-co-monomer) NPs exhibit an enhanced binding capacity toward glycoproteins by an additional functional monomer complementary to the surface presentation of the target protein. The effects of monomer ratio of AAPBA to hydrophilic comonomers on the binding of glycoproteins are systematically investigated. The morphology, structure, and composition of all the synthesized microspheres are characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM). The hydrophilic Fe3O4@P(AAPBA-co-monomer) microspheres show an excellent performance in the separation of glycoproteins with high binding capacity; And strong magnetic response allows them to be easily separated from solution in the presence of an external magnetic field. Moreover, both synthetic Fe3O4@P(AAPBA) and copolymeric NPs show good adsorption to glycoproteins in physiological conditions (pH 7.4). The Fe3O4@P(AAPBA-co-monomer) NPs are successfully utilized to selectively capture and identify the low-abundance glycopeptides from the tryptic digest of horseradish peroxidase (HRP). In addition, the selective isolation and enrichment of glycoproteins from the egg white samples at physiological condition is obtained by Fe3O4@P(AAPBA-co-monomer) NPs as adsorbents. SN - 1944-8252 UR - https://www.unboundmedicine.com/medline/citation/26479332/Tailor_Made_Boronic_Acid_Functionalized_Magnetic_Nanoparticles_with_a_Tunable_Polymer_Shell_Assisted_for_the_Selective_Enrichment_of_Glycoproteins/Glycopeptides_ L2 - https://dx.doi.org/10.1021/acsami.5b06445 DB - PRIME DP - Unbound Medicine ER -