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The protective effects of Ribes diacanthum Pall on cisplatin-induced nephrotoxicity in mice.
J Ethnopharmacol. 2016 Feb 03; 178:297-306.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Ribes diacanthum Pall. (Saxifragaceae), a Mongolian folk medicinal plant, was used to treat urinary system diseases. The present work aims to investigate the protective effects of Ribes diacanthum Pall (RDP) against cisplatin-induced nephrotoxicity.

METHODS

The renal injury was modeled by intraperitoneal injection of cisplatin for 5 consecutive days (5 mg/kg). Nephroprotection of RDP was investigated by oral administration of RDP aqueous extract at a daily dose of 40 mg/kg for 14 consecutive days, starting 7 days prior to cisplatin administration.

RESULTS

We demonstrated that pretreatment with RDP aqueous extract protected the mice from death induced by cisplatin administration. RDP treatment also significantly reduced blood urea nitrogen (BUN) and serum creatinine (Cr) levels observed in cisplatin-administrated mice. Histopathological analysis demonstrated that RDP administration protected cisplatin-induced renal tubular cell apoptosis. Further western blotting analysis revealed that RDP significantly reversed cisplatin-increased expression levels of cleaved-Caspase-3, Bax and cisplatin-decreased expression level of Bcl-2 in renal tissue. Finally, RDP markedly enhanced enzyme activities of reduced superoxide dismutase (SOD), Heme oxygenase 1 (HO-1) and catalase (CAT), suppressed lipid peroxidation as well as reactive oxygen species (ROS) production.

CONCLUSION

We concluded that RDP displayed nephroprotective effects against cisplatin-induced renal tubular cell apoptosis, possibly associated with both enhanced antioxidase activity and suppressed ROS generation. Given the major nephrotoxicity of cisplatin cancer chemotherapy, RDP might be a potential candidate for neoadjuvant chemotherapy.

Authors+Show Affiliations

Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, Jiangsu 211198, China.Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, Jiangsu 211198, China.Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, Jiangsu 211198, China; Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu 211198, China.Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, Jiangsu 211198, China; Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu 211198, China.Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, Jiangsu 211198, China.Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, Jiangsu 211198, China; Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu 211198, China. Electronic address: zycao1999@hotmail.com.Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, Jiangsu 211198, China; Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, Jiangsu 211198, China. Electronic address: boyangyu59@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26481606

Citation

Tilyek, Akhtolkhyn, et al. "The Protective Effects of Ribes Diacanthum Pall On Cisplatin-induced Nephrotoxicity in Mice." Journal of Ethnopharmacology, vol. 178, 2016, pp. 297-306.
Tilyek A, Chai C, Hou X, et al. The protective effects of Ribes diacanthum Pall on cisplatin-induced nephrotoxicity in mice. J Ethnopharmacol. 2016;178:297-306.
Tilyek, A., Chai, C., Hou, X., Zhou, B., Zhang, C., Cao, Z., & Yu, B. (2016). The protective effects of Ribes diacanthum Pall on cisplatin-induced nephrotoxicity in mice. Journal of Ethnopharmacology, 178, 297-306. https://doi.org/10.1016/j.jep.2015.10.003
Tilyek A, et al. The Protective Effects of Ribes Diacanthum Pall On Cisplatin-induced Nephrotoxicity in Mice. J Ethnopharmacol. 2016 Feb 3;178:297-306. PubMed PMID: 26481606.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The protective effects of Ribes diacanthum Pall on cisplatin-induced nephrotoxicity in mice. AU - Tilyek,Akhtolkhyn, AU - Chai,Chengzhi, AU - Hou,Xiaoli, AU - Zhou,Baoping, AU - Zhang,Chunlei, AU - Cao,Zhengyu, AU - Yu,Boyang, Y1 - 2015/10/17/ PY - 2015/04/29/received PY - 2015/09/26/revised PY - 2015/10/02/accepted PY - 2015/10/21/entrez PY - 2015/10/21/pubmed PY - 2016/10/16/medline KW - Apoptosis KW - Cisplatin KW - Nephrotoxicity/Nephropathology KW - Oxidative stress KW - Ribes diacanthum Pall SP - 297 EP - 306 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 178 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Ribes diacanthum Pall. (Saxifragaceae), a Mongolian folk medicinal plant, was used to treat urinary system diseases. The present work aims to investigate the protective effects of Ribes diacanthum Pall (RDP) against cisplatin-induced nephrotoxicity. METHODS: The renal injury was modeled by intraperitoneal injection of cisplatin for 5 consecutive days (5 mg/kg). Nephroprotection of RDP was investigated by oral administration of RDP aqueous extract at a daily dose of 40 mg/kg for 14 consecutive days, starting 7 days prior to cisplatin administration. RESULTS: We demonstrated that pretreatment with RDP aqueous extract protected the mice from death induced by cisplatin administration. RDP treatment also significantly reduced blood urea nitrogen (BUN) and serum creatinine (Cr) levels observed in cisplatin-administrated mice. Histopathological analysis demonstrated that RDP administration protected cisplatin-induced renal tubular cell apoptosis. Further western blotting analysis revealed that RDP significantly reversed cisplatin-increased expression levels of cleaved-Caspase-3, Bax and cisplatin-decreased expression level of Bcl-2 in renal tissue. Finally, RDP markedly enhanced enzyme activities of reduced superoxide dismutase (SOD), Heme oxygenase 1 (HO-1) and catalase (CAT), suppressed lipid peroxidation as well as reactive oxygen species (ROS) production. CONCLUSION: We concluded that RDP displayed nephroprotective effects against cisplatin-induced renal tubular cell apoptosis, possibly associated with both enhanced antioxidase activity and suppressed ROS generation. Given the major nephrotoxicity of cisplatin cancer chemotherapy, RDP might be a potential candidate for neoadjuvant chemotherapy. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/26481606/The_protective_effects_of_Ribes_diacanthum_Pall_on_cisplatin_induced_nephrotoxicity_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)30164-1 DB - PRIME DP - Unbound Medicine ER -