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blaNDM-1 Carriage on IncR Plasmid in Enterobacteriaceae Strains.
Microb Drug Resist. 2016 Mar; 22(2):123-8.MD

Abstract

Four NDM-1-producing Enterobacteriaceae strains (three Klebsiella pneumoniae and one Citrobacter koseri) were isolated between 2009 and 2011 through a nationwide surveillance for carbapenem-resistant Enterobacteriaceae in Croatia to study the molecular genetic background of blaNDM and the responsible plasmid types. Phenotypically, the clinical strains proved to be multidrug resistant. All strains remained susceptible to tigecycline and colistin. The clinical strains harbored variable antibiotic resistance determinants, notably, blaNDM-1, blaTEM-1, blaSHV-1, blaSHV-12, blaOXA-1, blaOXA-9, blaCTX-M-15, blaCMY-4, qnrB1, and aac(6')Ib-cr in different combinations. Two K. pneumoniae belonged to sequence type ST15 and one strain to ST16. As for the plasmid types, C. koseri and one of the ST15 K. pneumoniae carried IncR, and the second ST15 K. pneumoniae carried IncR and colE. The K. pneumoniae ST16 strain hosted A/C and colE plasmids. The blaNDM-1 gene was detected on conjugative high-molecular-weight plasmids, namely, A/C and IncR types. It is noteworthy that this is the first description of K. pneumoniae ST16 expressing NDM-1 in Europe. Remarkably, our study underscores the importance of the IncR plasmid as a reservoir of multidrug resistance. To the best of our knowledge, the IncR plasmid carrying blaNDM-1 in C. koseri is reported for the first time.

Authors+Show Affiliations

1 Department of Diagnostics and Public Health, University of Verona , Verona, Italy . 2 Institute of Medical Microbiology, Semmelweis University , Budapest, Hungary .3 Department of Clinical Microbiology, University Hospital for Infectious Diseases , Zagreb, Croatia .3 Department of Clinical Microbiology, University Hospital for Infectious Diseases , Zagreb, Croatia .4 Institute of Public Health , Bjelovar, Croatia .5 Department of Clinical Microbiology and Hospital Infections, University Hospital Dubrava , Zagreb, Croatia .3 Department of Clinical Microbiology, University Hospital for Infectious Diseases , Zagreb, Croatia .1 Department of Diagnostics and Public Health, University of Verona , Verona, Italy .1 Department of Diagnostics and Public Health, University of Verona , Verona, Italy .

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26484384

Citation

Kocsis, Erika, et al. "BlaNDM-1 Carriage On IncR Plasmid in Enterobacteriaceae Strains." Microbial Drug Resistance (Larchmont, N.Y.), vol. 22, no. 2, 2016, pp. 123-8.
Kocsis E, Gužvinec M, Butić I, et al. BlaNDM-1 Carriage on IncR Plasmid in Enterobacteriaceae Strains. Microb Drug Resist. 2016;22(2):123-8.
Kocsis, E., Gužvinec, M., Butić, I., Krešić, S., Crnek, S. Š., Tambić, A., Cornaglia, G., & Mazzariol, A. (2016). BlaNDM-1 Carriage on IncR Plasmid in Enterobacteriaceae Strains. Microbial Drug Resistance (Larchmont, N.Y.), 22(2), 123-8. https://doi.org/10.1089/mdr.2015.0083
Kocsis E, et al. BlaNDM-1 Carriage On IncR Plasmid in Enterobacteriaceae Strains. Microb Drug Resist. 2016;22(2):123-8. PubMed PMID: 26484384.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - blaNDM-1 Carriage on IncR Plasmid in Enterobacteriaceae Strains. AU - Kocsis,Erika, AU - Gužvinec,Marija, AU - Butić,Iva, AU - Krešić,Sanja, AU - Crnek,Sandra Šestan, AU - Tambić,Arjana, AU - Cornaglia,Giuseppe, AU - Mazzariol,Annarita, Y1 - 2015/10/20/ PY - 2015/10/21/entrez PY - 2015/10/21/pubmed PY - 2016/12/15/medline SP - 123 EP - 8 JF - Microbial drug resistance (Larchmont, N.Y.) JO - Microb. Drug Resist. VL - 22 IS - 2 N2 - Four NDM-1-producing Enterobacteriaceae strains (three Klebsiella pneumoniae and one Citrobacter koseri) were isolated between 2009 and 2011 through a nationwide surveillance for carbapenem-resistant Enterobacteriaceae in Croatia to study the molecular genetic background of blaNDM and the responsible plasmid types. Phenotypically, the clinical strains proved to be multidrug resistant. All strains remained susceptible to tigecycline and colistin. The clinical strains harbored variable antibiotic resistance determinants, notably, blaNDM-1, blaTEM-1, blaSHV-1, blaSHV-12, blaOXA-1, blaOXA-9, blaCTX-M-15, blaCMY-4, qnrB1, and aac(6')Ib-cr in different combinations. Two K. pneumoniae belonged to sequence type ST15 and one strain to ST16. As for the plasmid types, C. koseri and one of the ST15 K. pneumoniae carried IncR, and the second ST15 K. pneumoniae carried IncR and colE. The K. pneumoniae ST16 strain hosted A/C and colE plasmids. The blaNDM-1 gene was detected on conjugative high-molecular-weight plasmids, namely, A/C and IncR types. It is noteworthy that this is the first description of K. pneumoniae ST16 expressing NDM-1 in Europe. Remarkably, our study underscores the importance of the IncR plasmid as a reservoir of multidrug resistance. To the best of our knowledge, the IncR plasmid carrying blaNDM-1 in C. koseri is reported for the first time. SN - 1931-8448 UR - https://www.unboundmedicine.com/medline/citation/26484384/blaNDM_1_Carriage_on_IncR_Plasmid_in_Enterobacteriaceae_Strains_ L2 - https://www.liebertpub.com/doi/full/10.1089/mdr.2015.0083?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -