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Detection of MRSA ST3061-t843-mecC and ST398-t011-mecA in white stork nestlings exposed to human residues.
J Antimicrob Chemother. 2016 Jan; 71(1):53-7.JA

Abstract

OBJECTIVES

The objective of this study was to analyse the prevalence of tracheal carriage of Staphylococcus aureus/MRSA in storks and to study the resistance and virulence genes in the obtained isolates.

METHODS

Tracheal samples from 92 stork nestlings of two landfill-associated and two natural-habitat colonies were inoculated in specific media for S. aureus and MRSA recovery. Antimicrobial susceptibility was tested, and the presence of resistance, virulence and immune evasion cluster (IEC) genes was analysed by PCR. S. aureus isolates were characterized by spa and agr typing. Staphylococcal cassette chromosome (SCC) mec type was determined for mecC-positive isolates, and MLST was performed for 17 selected S. aureus isolates.

RESULTS

S. aureus isolates were identified in 32/92 samples (34.8%), and 38 isolates were recovered. The prevalence of S. aureus was higher in nestlings from landfills (24/43, 55.8%) than in those from natural habitats (8/49, 16.3%). Three birds from landfill-associated colonies carried MRSA, two with mecA-positive strains [clonal complex (CC) 5-spa-t002 and CC398-spa-t011] and one with a mecC-positive strain [sequence type (ST) 3061-CC130-spa-t843-agr-III-SCCmecXI). None of the MRSA isolates presented IEC genes. Thirty-five MSSA isolates, which showed 18 different spa types (ascribed to CC5, CC7, CC22, CC30, CC45, CC59, CC133 and CC398), were obtained. The agr types detected were I (63%), II (29%) and III (8%). Resistance and virulence genes identified in MSSA were blaZ (n = 25), erm(T) (n = 9), erm(A) (n = 1), tet(M) (n = 2), fexA (n = 3), str (n = 2), tst (n = 2), eta (n = 1) and cna (n = 15). The IEC types B, C, D and G were found in MSSA isolates, and two new STs were identified (ST3060 and ST3061).

CONCLUSIONS

White storks are frequently tracheal carriers of S. aureus, including ST398 isolates. MRSA isolates of lineages CC398-mecA and CC130-mecC were detected in storks from landfill-associated colonies exposed to human residues.

Authors+Show Affiliations

Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.SaBio, Spanish Wildlife Research Institute IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.SaBio, Spanish Wildlife Research Institute IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.SaBio, Spanish Wildlife Research Institute IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.SaBio, Spanish Wildlife Research Institute IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain carmen.torres@unirioja.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26490014

Citation

Gómez, Paula, et al. "Detection of MRSA ST3061-t843-mecC and ST398-t011-mecA in White Stork Nestlings Exposed to Human Residues." The Journal of Antimicrobial Chemotherapy, vol. 71, no. 1, 2016, pp. 53-7.
Gómez P, Lozano C, Camacho MC, et al. Detection of MRSA ST3061-t843-mecC and ST398-t011-mecA in white stork nestlings exposed to human residues. J Antimicrob Chemother. 2016;71(1):53-7.
Gómez, P., Lozano, C., Camacho, M. C., Lima-Barbero, J. F., Hernández, J. M., Zarazaga, M., Höfle, Ú., & Torres, C. (2016). Detection of MRSA ST3061-t843-mecC and ST398-t011-mecA in white stork nestlings exposed to human residues. The Journal of Antimicrobial Chemotherapy, 71(1), 53-7. https://doi.org/10.1093/jac/dkv314
Gómez P, et al. Detection of MRSA ST3061-t843-mecC and ST398-t011-mecA in White Stork Nestlings Exposed to Human Residues. J Antimicrob Chemother. 2016;71(1):53-7. PubMed PMID: 26490014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of MRSA ST3061-t843-mecC and ST398-t011-mecA in white stork nestlings exposed to human residues. AU - Gómez,Paula, AU - Lozano,Carmen, AU - Camacho,Mari Cruz, AU - Lima-Barbero,José-Francisco, AU - Hernández,José-Manuel, AU - Zarazaga,Myriam, AU - Höfle,Úrsula, AU - Torres,Carmen, Y1 - 2015/10/21/ PY - 2015/04/09/received PY - 2015/08/31/accepted PY - 2015/10/23/entrez PY - 2015/10/23/pubmed PY - 2016/10/7/medline SP - 53 EP - 7 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 71 IS - 1 N2 - OBJECTIVES: The objective of this study was to analyse the prevalence of tracheal carriage of Staphylococcus aureus/MRSA in storks and to study the resistance and virulence genes in the obtained isolates. METHODS: Tracheal samples from 92 stork nestlings of two landfill-associated and two natural-habitat colonies were inoculated in specific media for S. aureus and MRSA recovery. Antimicrobial susceptibility was tested, and the presence of resistance, virulence and immune evasion cluster (IEC) genes was analysed by PCR. S. aureus isolates were characterized by spa and agr typing. Staphylococcal cassette chromosome (SCC) mec type was determined for mecC-positive isolates, and MLST was performed for 17 selected S. aureus isolates. RESULTS: S. aureus isolates were identified in 32/92 samples (34.8%), and 38 isolates were recovered. The prevalence of S. aureus was higher in nestlings from landfills (24/43, 55.8%) than in those from natural habitats (8/49, 16.3%). Three birds from landfill-associated colonies carried MRSA, two with mecA-positive strains [clonal complex (CC) 5-spa-t002 and CC398-spa-t011] and one with a mecC-positive strain [sequence type (ST) 3061-CC130-spa-t843-agr-III-SCCmecXI). None of the MRSA isolates presented IEC genes. Thirty-five MSSA isolates, which showed 18 different spa types (ascribed to CC5, CC7, CC22, CC30, CC45, CC59, CC133 and CC398), were obtained. The agr types detected were I (63%), II (29%) and III (8%). Resistance and virulence genes identified in MSSA were blaZ (n = 25), erm(T) (n = 9), erm(A) (n = 1), tet(M) (n = 2), fexA (n = 3), str (n = 2), tst (n = 2), eta (n = 1) and cna (n = 15). The IEC types B, C, D and G were found in MSSA isolates, and two new STs were identified (ST3060 and ST3061). CONCLUSIONS: White storks are frequently tracheal carriers of S. aureus, including ST398 isolates. MRSA isolates of lineages CC398-mecA and CC130-mecC were detected in storks from landfill-associated colonies exposed to human residues. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/26490014/Detection_of_MRSA_ST3061_t843_mecC_and_ST398_t011_mecA_in_white_stork_nestlings_exposed_to_human_residues_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkv314 DB - PRIME DP - Unbound Medicine ER -