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A Cultivated Form of a Red Seaweed (Chondrus crispus), Suppresses β-Amyloid-Induced Paralysis in Caenorhabditis elegans.
Mar Drugs. 2015 Oct 20; 13(10):6407-24.MD

Abstract

We report here the protective effects of a methanol extract from a cultivated strain of the red seaweed, Chondrus crispus, against β-amyloid-induced toxicity, in a transgenic Caenorhabditis elegans, expressing human Aβ1-42 gene. The methanol extract of C. crispus (CCE), delayed β-amyloid-induced paralysis, whereas the water extract (CCW) was not effective. The CCE treatment did not affect the transcript abundance of amy1; however, Western blot analysis revealed a significant decrease of Aβ species, as compared to untreated worms. The transcript abundance of stress response genes; sod3, hsp16.2 and skn1 increased in CCE-treated worms. Bioassay guided fractionation of the CCE yielded a fraction enriched in monogalactosyl diacylglycerols (MGDG) that significantly delayed the onset of β-amyloid-induced paralysis. Taken together, these results suggested that the cultivated strain of C. crispus, whilst providing dietary nutritional value, may also have significant protective effects against β-amyloid-induced toxicity in C. elegans, partly through reduced β-amyloid species, up-regulation of stress induced genes and reduced accumulation of reactive oxygen species (ROS).

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Authors+Show Affiliations

Sangha JS
Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, PO Box 550, Truro, NS B2N 5E3, Canada. jatinder.sangha@dal.ca.
Wally O
Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, PO Box 550, Truro, NS B2N 5E3, Canada. owenwally@gmail.com.
Banskota AH
Aquatic and Crop Resources Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. arjun.banskota@nrc-cnrc.gc.ca.
Stefanova R
Aquatic and Crop Resources Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. Roumiana.Stefanova@nrc.gc.ca.
Hafting JT
Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, NS B3B 1X8, Canada. jhafting@acadian.ca.
Critchley AT
Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, NS B3B 1X8, Canada. Alan.Critchley@acadian.ca.
Prithiviraj B
Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, PO Box 550, Truro, NS B2N 5E3, Canada. bprithiviraj@dal.ca.

MeSH

Amyloid beta-PeptidesAnimalsAnimals, Genetically ModifiedBlotting, WesternCaenorhabditis elegansChondrusHumansMethanolParalysisPlant ExtractsReactive Oxygen SpeciesStress, PhysiologicalUp-Regulation

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26492254

Citation

Sangha, Jatinder Singh, et al. "A Cultivated Form of a Red Seaweed (Chondrus Crispus), Suppresses β-Amyloid-Induced Paralysis in Caenorhabditis Elegans." Marine Drugs, vol. 13, no. 10, 2015, pp. 6407-24.
Sangha JS, Wally O, Banskota AH, et al. A Cultivated Form of a Red Seaweed (Chondrus crispus), Suppresses β-Amyloid-Induced Paralysis in Caenorhabditis elegans. Mar Drugs. 2015;13(10):6407-24.
Sangha, J. S., Wally, O., Banskota, A. H., Stefanova, R., Hafting, J. T., Critchley, A. T., & Prithiviraj, B. (2015). A Cultivated Form of a Red Seaweed (Chondrus crispus), Suppresses β-Amyloid-Induced Paralysis in Caenorhabditis elegans. Marine Drugs, 13(10), 6407-24. https://doi.org/10.3390/md13106407
Sangha JS, et al. A Cultivated Form of a Red Seaweed (Chondrus Crispus), Suppresses β-Amyloid-Induced Paralysis in Caenorhabditis Elegans. Mar Drugs. 2015 Oct 20;13(10):6407-24. PubMed PMID: 26492254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Cultivated Form of a Red Seaweed (Chondrus crispus), Suppresses β-Amyloid-Induced Paralysis in Caenorhabditis elegans. AU - Sangha,Jatinder Singh, AU - Wally,Owen, AU - Banskota,Arjun H, AU - Stefanova,Roumiana, AU - Hafting,Jeff T, AU - Critchley,Alan T, AU - Prithiviraj,Balakrishnan, Y1 - 2015/10/20/ PY - 2015/06/15/received PY - 2015/09/23/revised PY - 2015/09/30/accepted PY - 2015/10/23/entrez PY - 2015/10/23/pubmed PY - 2016/7/9/medline KW - Caenorhabditis elegans KW - cultivated Chondrus crispus KW - glycolipid KW - monogalactosyl diacylglycerol (MGDG) KW - neuroprotection KW - red seaweeds KW - β-amyloid SP - 6407 EP - 24 JF - Marine drugs JO - Mar Drugs VL - 13 IS - 10 N2 - We report here the protective effects of a methanol extract from a cultivated strain of the red seaweed, Chondrus crispus, against β-amyloid-induced toxicity, in a transgenic Caenorhabditis elegans, expressing human Aβ1-42 gene. The methanol extract of C. crispus (CCE), delayed β-amyloid-induced paralysis, whereas the water extract (CCW) was not effective. The CCE treatment did not affect the transcript abundance of amy1; however, Western blot analysis revealed a significant decrease of Aβ species, as compared to untreated worms. The transcript abundance of stress response genes; sod3, hsp16.2 and skn1 increased in CCE-treated worms. Bioassay guided fractionation of the CCE yielded a fraction enriched in monogalactosyl diacylglycerols (MGDG) that significantly delayed the onset of β-amyloid-induced paralysis. Taken together, these results suggested that the cultivated strain of C. crispus, whilst providing dietary nutritional value, may also have significant protective effects against β-amyloid-induced toxicity in C. elegans, partly through reduced β-amyloid species, up-regulation of stress induced genes and reduced accumulation of reactive oxygen species (ROS). SN - 1660-3397 UR - https://www.unboundmedicine.com/medline/citation/26492254/A_Cultivated_Form_of_a_Red_Seaweed__Chondrus_crispus__Suppresses_β_Amyloid_Induced_Paralysis_in_Caenorhabditis_elegans_ L2 - https://www.mdpi.com/resolver?pii=md13106407 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓11 ↑29]

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