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Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens.
Appl Microbiol Biotechnol. 2016 Jan; 100(2):781-96.AM

Abstract

Strain breeding is much less advanced in the edible and medicinal species Agaricus subrufescens than in Agaricus bisporus, the button mushroom. Both species have a unifactorial system of sexual incompatibility, a mating type locus tightly linked to a centromere, and basidia producing both homokaryotic (n) and heterokaryotic (n + n) spores. In A. bisporus, breeding is mainly based on direct selection among the heterokaryotic offspring and on hybridization between homokaryotic offspring. The parental heterozygosity is highly maintained in the heterokaryotic offspring due to suppression of recombination and preferential pairing in the spores of nuclei, each one per second meiotic divisions; such "non-sister nuclei" heterokaryons are fertile. In A. subrufescens, recent studies revealed that recombination is not suppressed and that nuclei from the same second meiotic division can also be paired in a spore that give rise to a "sister nuclei" heterokaryon in which the nuclei bear the same mating type allele. The objective of the present work was to investigate the potential function of the different categories of spores in A. subrufescens and their possible use in a genetic breeding program. Using eight co-dominant molecular markers, we found that half of the offspring of the A. subrufescens strain WC837 were heterokaryotic, one quarter of them being sister nuclei heterokaryons. These heterokaryons were infertile and behaved like homokaryons, being even able to cross between each other. In contrast, non-sister nuclei heterokaryons could fruit but inconsistently due to inbreeding depression. Potential roles of these two categories of heterokaryons in nature and consequences for strain breeding are discussed.

Authors+Show Affiliations

INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, CS 20032, F-33882, Villenave d'Ornon Cedex, France. Departamento de Biologia, UFLA, Universidade Federal de Lavras, C.P. 3037, 37200-000, Lavras, MG, Brazil.INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, CS 20032, F-33882, Villenave d'Ornon Cedex, France.INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, CS 20032, F-33882, Villenave d'Ornon Cedex, France.Departamento de Biologia, UFLA, Universidade Federal de Lavras, C.P. 3037, 37200-000, Lavras, MG, Brazil.INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, CS 20032, F-33882, Villenave d'Ornon Cedex, France.INRA, UR1264 MycSA, Mycologie et Sécurité des Aliments, CS 20032, F-33882, Villenave d'Ornon Cedex, France. callac@bordeaux.inra.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26497018

Citation

Rocha de Brito, Manuela, et al. "Spore Behaviors Reveal a Category of Mating-competent Infertile Heterokaryons in the Offspring of the Medicinal Fungus Agaricus Subrufescens." Applied Microbiology and Biotechnology, vol. 100, no. 2, 2016, pp. 781-96.
Rocha de Brito M, Foulongne-Oriol M, Moinard M, et al. Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens. Appl Microbiol Biotechnol. 2016;100(2):781-96.
Rocha de Brito, M., Foulongne-Oriol, M., Moinard, M., Souza Dias, E., Savoie, J. M., & Callac, P. (2016). Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens. Applied Microbiology and Biotechnology, 100(2), 781-96. https://doi.org/10.1007/s00253-015-7070-7
Rocha de Brito M, et al. Spore Behaviors Reveal a Category of Mating-competent Infertile Heterokaryons in the Offspring of the Medicinal Fungus Agaricus Subrufescens. Appl Microbiol Biotechnol. 2016;100(2):781-96. PubMed PMID: 26497018.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens. AU - Rocha de Brito,Manuela, AU - Foulongne-Oriol,Marie, AU - Moinard,Magalie, AU - Souza Dias,Eustáquio, AU - Savoie,Jean-Michel, AU - Callac,Philippe, Y1 - 2015/10/26/ PY - 2015/05/28/received PY - 2015/10/15/accepted PY - 2015/10/06/revised PY - 2015/10/27/entrez PY - 2015/10/27/pubmed PY - 2016/11/1/medline KW - Breeding program KW - Buller phenomenon KW - Life cycle KW - Mushroom KW - Pseudohomothallism SP - 781 EP - 96 JF - Applied microbiology and biotechnology JO - Appl. Microbiol. Biotechnol. VL - 100 IS - 2 N2 - Strain breeding is much less advanced in the edible and medicinal species Agaricus subrufescens than in Agaricus bisporus, the button mushroom. Both species have a unifactorial system of sexual incompatibility, a mating type locus tightly linked to a centromere, and basidia producing both homokaryotic (n) and heterokaryotic (n + n) spores. In A. bisporus, breeding is mainly based on direct selection among the heterokaryotic offspring and on hybridization between homokaryotic offspring. The parental heterozygosity is highly maintained in the heterokaryotic offspring due to suppression of recombination and preferential pairing in the spores of nuclei, each one per second meiotic divisions; such "non-sister nuclei" heterokaryons are fertile. In A. subrufescens, recent studies revealed that recombination is not suppressed and that nuclei from the same second meiotic division can also be paired in a spore that give rise to a "sister nuclei" heterokaryon in which the nuclei bear the same mating type allele. The objective of the present work was to investigate the potential function of the different categories of spores in A. subrufescens and their possible use in a genetic breeding program. Using eight co-dominant molecular markers, we found that half of the offspring of the A. subrufescens strain WC837 were heterokaryotic, one quarter of them being sister nuclei heterokaryons. These heterokaryons were infertile and behaved like homokaryons, being even able to cross between each other. In contrast, non-sister nuclei heterokaryons could fruit but inconsistently due to inbreeding depression. Potential roles of these two categories of heterokaryons in nature and consequences for strain breeding are discussed. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/26497018/Spore_behaviors_reveal_a_category_of_mating_competent_infertile_heterokaryons_in_the_offspring_of_the_medicinal_fungus_Agaricus_subrufescens_ L2 - https://dx.doi.org/10.1007/s00253-015-7070-7 DB - PRIME DP - Unbound Medicine ER -