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Identification of novel alleles of the rice blast resistance gene Pi54.
Sci Rep. 2015 Oct 26; 5:15678.SR

Abstract

Rice blast is one of the most devastating rice diseases and continuous resistance breeding is required to control the disease. The rice blast resistance gene Pi54 initially identified in an Indian cultivar confers broad-spectrum resistance in India. We explored the allelic diversity of the Pi54 gene among 885 Indian rice genotypes that were found resistant in our screening against field mixture of naturally existing M. oryzae strains as well as against five unique strains. These genotypes are also annotated as rice blast resistant in the International Rice Genebank database. Sequence-based allele mining was used to amplify and clone the Pi54 allelic variants. Nine new alleles of Pi54 were identified based on the nucleotide sequence comparison to the Pi54 reference sequence as well as to already known Pi54 alleles. DNA sequence analysis of the newly identified Pi54 alleles revealed several single polymorphic sites, three double deletions and an eight base pair deletion. A SNP-rich region was found between a tyrosine kinase phosphorylation site and the nucleotide binding site (NBS) domain. Together, the newly identified Pi54 alleles expand the allelic series and are candidates for rice blast resistance breeding programs.

Authors+Show Affiliations

Plant Biotechnology, Department of Biology, ETH Zurich (Swiss Federal Institute of Technology), Zurich, Switzerland.Plant Biotechnology, Department of Biology, ETH Zurich (Swiss Federal Institute of Technology), Zurich, Switzerland.Plant Biotechnology, Department of Biology, ETH Zurich (Swiss Federal Institute of Technology), Zurich, Switzerland.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26498172

Citation

Vasudevan, Kumar, et al. "Identification of Novel Alleles of the Rice Blast Resistance Gene Pi54." Scientific Reports, vol. 5, 2015, p. 15678.
Vasudevan K, Gruissem W, Bhullar NK. Identification of novel alleles of the rice blast resistance gene Pi54. Sci Rep. 2015;5:15678.
Vasudevan, K., Gruissem, W., & Bhullar, N. K. (2015). Identification of novel alleles of the rice blast resistance gene Pi54. Scientific Reports, 5, 15678. https://doi.org/10.1038/srep15678
Vasudevan K, Gruissem W, Bhullar NK. Identification of Novel Alleles of the Rice Blast Resistance Gene Pi54. Sci Rep. 2015 Oct 26;5:15678. PubMed PMID: 26498172.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of novel alleles of the rice blast resistance gene Pi54. AU - Vasudevan,Kumar, AU - Gruissem,Wilhelm, AU - Bhullar,Navreet K, Y1 - 2015/10/26/ PY - 2015/04/21/received PY - 2015/10/01/accepted PY - 2015/10/27/entrez PY - 2015/10/27/pubmed PY - 2016/8/31/medline SP - 15678 EP - 15678 JF - Scientific reports JO - Sci Rep VL - 5 N2 - Rice blast is one of the most devastating rice diseases and continuous resistance breeding is required to control the disease. The rice blast resistance gene Pi54 initially identified in an Indian cultivar confers broad-spectrum resistance in India. We explored the allelic diversity of the Pi54 gene among 885 Indian rice genotypes that were found resistant in our screening against field mixture of naturally existing M. oryzae strains as well as against five unique strains. These genotypes are also annotated as rice blast resistant in the International Rice Genebank database. Sequence-based allele mining was used to amplify and clone the Pi54 allelic variants. Nine new alleles of Pi54 were identified based on the nucleotide sequence comparison to the Pi54 reference sequence as well as to already known Pi54 alleles. DNA sequence analysis of the newly identified Pi54 alleles revealed several single polymorphic sites, three double deletions and an eight base pair deletion. A SNP-rich region was found between a tyrosine kinase phosphorylation site and the nucleotide binding site (NBS) domain. Together, the newly identified Pi54 alleles expand the allelic series and are candidates for rice blast resistance breeding programs. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/26498172/Identification_of_novel_alleles_of_the_rice_blast_resistance_gene_Pi54_ L2 - https://doi.org/10.1038/srep15678 DB - PRIME DP - Unbound Medicine ER -