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Involvement of epithelial-to-mesenchymal transition and associated transforming growth factor-β/Smad signaling in paraquat-induced pulmonary fibrosis.
Mol Med Rep. 2015 Dec; 12(6):7979-84.MM

Abstract

Paraquat (PQ) is a highly toxic herbicide which is able to induce pulmonary fibrosis in humans and animals. The epithelial‑to‑mesenchymal transition (EMT) was demonstrated to be an important factor in pulmonary fibrosis. However, it has remained elusive whether PQ induces pulmonary fibrosis via EMT, which was therefore investigated in the present study. In addition, the underlying mechanisms of PQ‑induced EMT were examined in vitro. Hematoxylin and eosin staining of rat lung tissues demonstrated that PQ induced pulmonary fibrosis in vivo. Western blot analysis then revealed that the expression of epithelial cell marker E‑cadherin was significantly decreased, while the expression of mesenchymal markers α‑smooth‑muscle actin and vimentin was significantly increased in rat lung tissues and A549 cells following PQ treatment. Transforming growth factor (TGF)‑β/Smad signaling was also induced by PQ as evidenced by increased expression of TGF‑β1 and Smad2. However, PQ‑induced EMT in A549 cells was abolished by transfection with TGF‑β1‑specific small hairpin RNA. In conclusion, the present study demonstrated that PQ induced EMT in vivo and in vitro, which may be an important process in the development of PQ‑induced pulmonary fibrosis. In addition, TGF-β/Smad signaling was involved in PQ-induced EMT.

Authors+Show Affiliations

Department of Nephrology, Tianjin First Center Hospital, Tianjin 300192, P.R. China.Department of Emergency Medicine, Tianjin First Center Hospital, Tianjin 300192, P.R. China.Department of Nephrology, Tianjin First Center Hospital, Tianjin 300192, P.R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26499763

Citation

Han, Ying-Ying, et al. "Involvement of Epithelial-to-mesenchymal Transition and Associated Transforming Growth factor-β/Smad Signaling in Paraquat-induced Pulmonary Fibrosis." Molecular Medicine Reports, vol. 12, no. 6, 2015, pp. 7979-84.
Han YY, Shen P, Chang WX. Involvement of epithelial-to-mesenchymal transition and associated transforming growth factor-β/Smad signaling in paraquat-induced pulmonary fibrosis. Mol Med Rep. 2015;12(6):7979-84.
Han, Y. Y., Shen, P., & Chang, W. X. (2015). Involvement of epithelial-to-mesenchymal transition and associated transforming growth factor-β/Smad signaling in paraquat-induced pulmonary fibrosis. Molecular Medicine Reports, 12(6), 7979-84. https://doi.org/10.3892/mmr.2015.4454
Han YY, Shen P, Chang WX. Involvement of Epithelial-to-mesenchymal Transition and Associated Transforming Growth factor-β/Smad Signaling in Paraquat-induced Pulmonary Fibrosis. Mol Med Rep. 2015;12(6):7979-84. PubMed PMID: 26499763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of epithelial-to-mesenchymal transition and associated transforming growth factor-β/Smad signaling in paraquat-induced pulmonary fibrosis. AU - Han,Ying-Ying, AU - Shen,Peng, AU - Chang,Wen-Xiu, Y1 - 2015/10/19/ PY - 2014/12/30/received PY - 2015/09/25/accepted PY - 2015/10/27/entrez PY - 2015/10/27/pubmed PY - 2016/9/13/medline SP - 7979 EP - 84 JF - Molecular medicine reports JO - Mol Med Rep VL - 12 IS - 6 N2 - Paraquat (PQ) is a highly toxic herbicide which is able to induce pulmonary fibrosis in humans and animals. The epithelial‑to‑mesenchymal transition (EMT) was demonstrated to be an important factor in pulmonary fibrosis. However, it has remained elusive whether PQ induces pulmonary fibrosis via EMT, which was therefore investigated in the present study. In addition, the underlying mechanisms of PQ‑induced EMT were examined in vitro. Hematoxylin and eosin staining of rat lung tissues demonstrated that PQ induced pulmonary fibrosis in vivo. Western blot analysis then revealed that the expression of epithelial cell marker E‑cadherin was significantly decreased, while the expression of mesenchymal markers α‑smooth‑muscle actin and vimentin was significantly increased in rat lung tissues and A549 cells following PQ treatment. Transforming growth factor (TGF)‑β/Smad signaling was also induced by PQ as evidenced by increased expression of TGF‑β1 and Smad2. However, PQ‑induced EMT in A549 cells was abolished by transfection with TGF‑β1‑specific small hairpin RNA. In conclusion, the present study demonstrated that PQ induced EMT in vivo and in vitro, which may be an important process in the development of PQ‑induced pulmonary fibrosis. In addition, TGF-β/Smad signaling was involved in PQ-induced EMT. SN - 1791-3004 UR - https://www.unboundmedicine.com/medline/citation/26499763/Involvement_of_epithelial_to_mesenchymal_transition_and_associated_transforming_growth_factor_β/Smad_signaling_in_paraquat_induced_pulmonary_fibrosis_ L2 - http://www.spandidos-publications.com/mmr/12/6/7979 DB - PRIME DP - Unbound Medicine ER -