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[Effects of Acupuncture Intervention on Levels of T Lymphocyte Subsets in Plasma and Thymus in Stress-induced Anxiety Rats].
Zhen Ci Yan Jiu. 2015 Aug; 40(4):265-9.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) intervention on the levels of CD 4+ and CD 8+ lymphocytes in the plasma and thymus in anxiety disorder rats, so as to explore its mechanism underlying favorable regulation of immune function.

METHODS

Thirty-four SD rats were randomly divided into control (n = 10) , model (n = 12) and EA (n = 12) groups. The anxiety model was established by using chronic unpredictable emotional stress stimulation (fasting, water-deprivation, shaking, tail-clamping, forced warm- and cool-water swimming, electrical shock stimulation, etc.) for 15 days. EA (15 Hz/ 25 Hz) was applied to "Neiguan" (PC 6) and "Shenmen" (HT 7) for 15 min, once every other day for 15 days. The anxiety-like behavior was measured by elevated plus maze test. Anxiety reduction in the plus-maze is indicated by an increase in the proportion of time spent in the open arms (time in open arms/total time in open or closed arms) , and an increase in the proportion of entries into the open arms (entries into open arms/total entries into open or closed arms). While the contents and expression of CD 4+ and CD 8+ in the plasma and thymus tissues were measured by ELISA and immunohistochemistry, respectively.

RESULTS

The values of proportions of open-arm entries (OE%) and the content of plasma CD 4+ lymphocytes were obviously lower in the model group than in the control group (P < 0.05), and were significantly higher in the EA group than in the model group (P < 0.05). Whereas, the content of plasma CD 8+ lymphocytes was obviously higher in the model group than in the control group (P < 0.05) and markedly down-regulated in the EA group after the treatment (P < 0.05). The ratio of CD 4+/CD 8+ was decreased in the model group in comparison with the control group (P = 0.054), and was significantly up-regulated in the EA group after EA stimulation (P < 0.01). Compared with the control group, the expression levels (optical density, OD values) of CD 4+ and CD 8+ lymphocytes in the thymus tissue were obviously decreased in the model group (P < 0.01). After EA, the expression levels of thymus CD 4+ and CD 8+ were remarkably up-regulated in the EA group (P < 0.05). No significant changes were found in proportion of open-arm time (OT%, P > 0.05).

CONCLUSION

EA can effectively relieve anxiety disorder in anxiety rats, which may be related to its effects in up-regulating plasma CD 4+ , thymus CD 4+ and CD 8' levels as well as CD 4+/CD 8+ ratio, and down-regulating plasma CD 8+ content.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26502538

Citation

Huang, Wen-qin, et al. "[Effects of Acupuncture Intervention On Levels of T Lymphocyte Subsets in Plasma and Thymus in Stress-induced Anxiety Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 40, no. 4, 2015, pp. 265-9.
Huang WQ, Zhou QZ, Liu XG, et al. [Effects of Acupuncture Intervention on Levels of T Lymphocyte Subsets in Plasma and Thymus in Stress-induced Anxiety Rats]. Zhen Ci Yan Jiu. 2015;40(4):265-9.
Huang, W. Q., Zhou, Q. Z., Liu, X. G., Wei, D. N., He, W., Zhang, X. D., & Peng, X. H. (2015). [Effects of Acupuncture Intervention on Levels of T Lymphocyte Subsets in Plasma and Thymus in Stress-induced Anxiety Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 40(4), 265-9.
Huang WQ, et al. [Effects of Acupuncture Intervention On Levels of T Lymphocyte Subsets in Plasma and Thymus in Stress-induced Anxiety Rats]. Zhen Ci Yan Jiu. 2015;40(4):265-9. PubMed PMID: 26502538.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of Acupuncture Intervention on Levels of T Lymphocyte Subsets in Plasma and Thymus in Stress-induced Anxiety Rats]. AU - Huang,Wen-qin, AU - Zhou,Qi-zhi, AU - Liu,Xu-guang, AU - Wei,Da-neng, AU - He,Wei, AU - Zhang,Xiao-dan, AU - Peng,Xiao-hua, PY - 2015/10/28/entrez PY - 2015/10/28/pubmed PY - 2015/12/23/medline SP - 265 EP - 9 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 40 IS - 4 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) intervention on the levels of CD 4+ and CD 8+ lymphocytes in the plasma and thymus in anxiety disorder rats, so as to explore its mechanism underlying favorable regulation of immune function. METHODS: Thirty-four SD rats were randomly divided into control (n = 10) , model (n = 12) and EA (n = 12) groups. The anxiety model was established by using chronic unpredictable emotional stress stimulation (fasting, water-deprivation, shaking, tail-clamping, forced warm- and cool-water swimming, electrical shock stimulation, etc.) for 15 days. EA (15 Hz/ 25 Hz) was applied to "Neiguan" (PC 6) and "Shenmen" (HT 7) for 15 min, once every other day for 15 days. The anxiety-like behavior was measured by elevated plus maze test. Anxiety reduction in the plus-maze is indicated by an increase in the proportion of time spent in the open arms (time in open arms/total time in open or closed arms) , and an increase in the proportion of entries into the open arms (entries into open arms/total entries into open or closed arms). While the contents and expression of CD 4+ and CD 8+ in the plasma and thymus tissues were measured by ELISA and immunohistochemistry, respectively. RESULTS: The values of proportions of open-arm entries (OE%) and the content of plasma CD 4+ lymphocytes were obviously lower in the model group than in the control group (P < 0.05), and were significantly higher in the EA group than in the model group (P < 0.05). Whereas, the content of plasma CD 8+ lymphocytes was obviously higher in the model group than in the control group (P < 0.05) and markedly down-regulated in the EA group after the treatment (P < 0.05). The ratio of CD 4+/CD 8+ was decreased in the model group in comparison with the control group (P = 0.054), and was significantly up-regulated in the EA group after EA stimulation (P < 0.01). Compared with the control group, the expression levels (optical density, OD values) of CD 4+ and CD 8+ lymphocytes in the thymus tissue were obviously decreased in the model group (P < 0.01). After EA, the expression levels of thymus CD 4+ and CD 8+ were remarkably up-regulated in the EA group (P < 0.05). No significant changes were found in proportion of open-arm time (OT%, P > 0.05). CONCLUSION: EA can effectively relieve anxiety disorder in anxiety rats, which may be related to its effects in up-regulating plasma CD 4+ , thymus CD 4+ and CD 8' levels as well as CD 4+/CD 8+ ratio, and down-regulating plasma CD 8+ content. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/26502538/[Effects_of_Acupuncture_Intervention_on_Levels_of_T_Lymphocyte_Subsets_in_Plasma_and_Thymus_in_Stress_induced_Anxiety_Rats]_ DB - PRIME DP - Unbound Medicine ER -