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[Effect of Electroacupuncture at "Zusanli" (ST 36), etc. on Gastrointestinal Motility and Expression of Ghrelin mRNA and Growth Hormone Secretagogue Receptor mRNA in Diabetic Gastroparesis Rats].
Zhen Ci Yan Jiu. 2015 Aug; 40(4):290-5.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) stimulation of "Zusanli" (ST 36), "Liangmen" (ST 21) and "Sanyinjiao" (SP 6) on gastrointestinal motility and expression of ghrelin and growth hormone secretagogue receptor (GHSR) mRNA in diabetic gastroparesis (diabetic gastroparesis, DGP) rats, so as to explore its mechanism underlying improvement of diabetic gastroparesis.

METHODS

Sixty SD rats were randomly and equally divided into normal control, model, EA acupoint, EA non-acupoint, medication (metoclopramide) groups (n = 12 rat/group). The DGP model was established by intraperitoneal injection of streptozotocin (STZ, 55 mg/kg, 2%) and raised by high-sugar and high-fat fodder irregularly for 8 weeks. EA was applied to "Zusanli" (ST 36), "Liangmen" (ST 21) and "Sanyinjiao" (SP 6), and non-acupoints (about 5 mm lateral to the 3 acupoints, respectively). Those rats of the medication group were given with metoclopramide (1 mL/100 g). The blood sugar level was measured by glucometer, and the urine sugar level measured by Tes-Tape [urine glucose test paper]. The intestinal propulsion rate (IPR) was identified as ratio of the phenolsulfonphthalein promoting distance and the total length of the small intestine, and the gastric emptying rate (GER) was assessed by gavage of a liquid test meal (1.5 mL) containing a nonabsorbable marker (0. 5 mg/mL of phenol red in 5% glucose solution) and identification after being sacrificed 72 h after modeling. The expression levels of ghrelin mRNA and GHSR mRNA of gastric antrum tissue were examined by Quantitative real-time PCR.

RESULTS

In comparison with the normal control group, the blood sugar and urine sugar levels of the model group was significantly increased in the model group (P < 0.01), while the GER and IPR and the expression level of ghrelin mRNA and GHSR mRNA in the gastric antrum were significantly decreased in the model group (P < 0.01, P < 0.05). Following EA stimulation of acupoint, the blood sugar and urine sugar levels were obviously down-regulated (P < 0.05), both GER and IPR, and ghrelin mRNA and GHSR mRNA expression levels in the EA-acupoint group were significantly up-regulated (P < 0.05, P < 0.01). No significant changes were found in both blood sugar and urine sugar levels, in both GER and IPR, and ghrelin mRNA and GHSR mRNA expression levels of both EA non-acupoint and medication groups after the intervention (P > 0.05). The GER and IPR were significantly higher in the EA acupoint group than in the EA non-acupoint group (P < 0.05, P < 0.01).

CONCLUSION

EA stimulation of ST 36, ST 21 and SP 6 can promote the gastrointestinal motility of DGP rats, which may be closely associated with its effects in up-regulating the expression level of ghrelin mRNA and GHSR mRNA in gastric antrum.

Authors

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Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26502542

Citation

Lin, Ya-ping, et al. "[Effect of Electroacupuncture at "Zusanli" (ST 36), Etc. On Gastrointestinal Motility and Expression of Ghrelin mRNA and Growth Hormone Secretagogue Receptor mRNA in Diabetic Gastroparesis Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 40, no. 4, 2015, pp. 290-5.
Lin YP, Wan QQ, Peng Y, et al. [Effect of Electroacupuncture at "Zusanli" (ST 36), etc. on Gastrointestinal Motility and Expression of Ghrelin mRNA and Growth Hormone Secretagogue Receptor mRNA in Diabetic Gastroparesis Rats]. Zhen Ci Yan Jiu. 2015;40(4):290-5.
Lin, Y. P., Wan, Q. Q., Peng, Y., He, F. E., & Shen, J. (2015). [Effect of Electroacupuncture at "Zusanli" (ST 36), etc. on Gastrointestinal Motility and Expression of Ghrelin mRNA and Growth Hormone Secretagogue Receptor mRNA in Diabetic Gastroparesis Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 40(4), 290-5.
Lin YP, et al. [Effect of Electroacupuncture at "Zusanli" (ST 36), Etc. On Gastrointestinal Motility and Expression of Ghrelin mRNA and Growth Hormone Secretagogue Receptor mRNA in Diabetic Gastroparesis Rats]. Zhen Ci Yan Jiu. 2015;40(4):290-5. PubMed PMID: 26502542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Electroacupuncture at "Zusanli" (ST 36), etc. on Gastrointestinal Motility and Expression of Ghrelin mRNA and Growth Hormone Secretagogue Receptor mRNA in Diabetic Gastroparesis Rats]. AU - Lin,Ya-ping, AU - Wan,Quan-quan, AU - Peng,Yan, AU - He,Feng-e, AU - Shen,Jing, PY - 2015/10/28/entrez PY - 2015/10/28/pubmed PY - 2015/12/23/medline SP - 290 EP - 5 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 40 IS - 4 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) stimulation of "Zusanli" (ST 36), "Liangmen" (ST 21) and "Sanyinjiao" (SP 6) on gastrointestinal motility and expression of ghrelin and growth hormone secretagogue receptor (GHSR) mRNA in diabetic gastroparesis (diabetic gastroparesis, DGP) rats, so as to explore its mechanism underlying improvement of diabetic gastroparesis. METHODS: Sixty SD rats were randomly and equally divided into normal control, model, EA acupoint, EA non-acupoint, medication (metoclopramide) groups (n = 12 rat/group). The DGP model was established by intraperitoneal injection of streptozotocin (STZ, 55 mg/kg, 2%) and raised by high-sugar and high-fat fodder irregularly for 8 weeks. EA was applied to "Zusanli" (ST 36), "Liangmen" (ST 21) and "Sanyinjiao" (SP 6), and non-acupoints (about 5 mm lateral to the 3 acupoints, respectively). Those rats of the medication group were given with metoclopramide (1 mL/100 g). The blood sugar level was measured by glucometer, and the urine sugar level measured by Tes-Tape [urine glucose test paper]. The intestinal propulsion rate (IPR) was identified as ratio of the phenolsulfonphthalein promoting distance and the total length of the small intestine, and the gastric emptying rate (GER) was assessed by gavage of a liquid test meal (1.5 mL) containing a nonabsorbable marker (0. 5 mg/mL of phenol red in 5% glucose solution) and identification after being sacrificed 72 h after modeling. The expression levels of ghrelin mRNA and GHSR mRNA of gastric antrum tissue were examined by Quantitative real-time PCR. RESULTS: In comparison with the normal control group, the blood sugar and urine sugar levels of the model group was significantly increased in the model group (P < 0.01), while the GER and IPR and the expression level of ghrelin mRNA and GHSR mRNA in the gastric antrum were significantly decreased in the model group (P < 0.01, P < 0.05). Following EA stimulation of acupoint, the blood sugar and urine sugar levels were obviously down-regulated (P < 0.05), both GER and IPR, and ghrelin mRNA and GHSR mRNA expression levels in the EA-acupoint group were significantly up-regulated (P < 0.05, P < 0.01). No significant changes were found in both blood sugar and urine sugar levels, in both GER and IPR, and ghrelin mRNA and GHSR mRNA expression levels of both EA non-acupoint and medication groups after the intervention (P > 0.05). The GER and IPR were significantly higher in the EA acupoint group than in the EA non-acupoint group (P < 0.05, P < 0.01). CONCLUSION: EA stimulation of ST 36, ST 21 and SP 6 can promote the gastrointestinal motility of DGP rats, which may be closely associated with its effects in up-regulating the expression level of ghrelin mRNA and GHSR mRNA in gastric antrum. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/26502542/[Effect_of_Electroacupuncture_at_"Zusanli"__ST_36__etc__on_Gastrointestinal_Motility_and_Expression_of_Ghrelin_mRNA_and_Growth_Hormone_Secretagogue_Receptor_mRNA_in_Diabetic_Gastroparesis_Rats]_ L2 - http://www.diseaseinfosearch.org/result/2998 DB - PRIME DP - Unbound Medicine ER -