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In vivo activity of vancomycin combined with colistin against multidrug-resistant strains of Acinetobacter baumannii in a Galleria mellonella model.
Infect Dis (Lond). 2016; 48(3):189-94.ID

Abstract

BACKGROUND

With increasing antibiotic resistance, the selection of effective treatment of A. baumannii infections is particularly challenging.

METHODS

This study assessed the activities of the combination of vancomycin and colistin combination in vitro and in vivo using a Galleria mellonella model against four colistin-susceptible or colistin-resistant A. baumannii strains.

RESULTS

In checkerboard assays, synergy was observed between vancomycin and colistin for all four strains tested (0.156 ≤ Fractional inhibitory concentration indices [FICI] ≤ 0.281). In time-kill assays, the combination showed continued bactericidal activity and synergy after 24 h for colistin-susceptible strains. For colistin-resistant strains, the combination resulted in bactericidal activity within 8 h, but sustained bacterial re-growth was then observed. Treatment of G. mellonella larvae infected with lethal doses of A. baumannii (except 19606R) resulted in significantly increased survival rates when vancomycin was given with colistin compared to colistin treatment alone (p < 0.05).

CONCLUSIONS

These findings suggest that regimens containing vancomycin may be useful for infections due to multidrug-resistant Acinetobacter baumannii.

Authors+Show Affiliations

a Department of Infectious Diseases.b Department of Dentistry , the First Affiliated Hospital of Anhui Medical University , Hefei , PR China.a Department of Infectious Diseases.c Institute of Bacterium Resistance, Anhui Medical University , Hefei , PR China. d Anhui Center for Surveillance of Bacterial Resistance , Hefei , PR China.a Department of Infectious Diseases.a Department of Infectious Diseases. c Institute of Bacterium Resistance, Anhui Medical University , Hefei , PR China. d Anhui Center for Surveillance of Bacterial Resistance , Hefei , PR China.a Department of Infectious Diseases. c Institute of Bacterium Resistance, Anhui Medical University , Hefei , PR China. d Anhui Center for Surveillance of Bacterial Resistance , Hefei , PR China. e Department of Infectious Diseases , Chaohu Hospital of Anhui Medical University , Hefei , PR China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26503239

Citation

Yang, Haifei, et al. "In Vivo Activity of Vancomycin Combined With Colistin Against Multidrug-resistant Strains of Acinetobacter Baumannii in a Galleria Mellonella Model." Infectious Diseases (London, England), vol. 48, no. 3, 2016, pp. 189-94.
Yang H, Lv N, Hu L, et al. In vivo activity of vancomycin combined with colistin against multidrug-resistant strains of Acinetobacter baumannii in a Galleria mellonella model. Infect Dis (Lond). 2016;48(3):189-94.
Yang, H., Lv, N., Hu, L., Liu, Y., Cheng, J., Ye, Y., & Li, J. (2016). In vivo activity of vancomycin combined with colistin against multidrug-resistant strains of Acinetobacter baumannii in a Galleria mellonella model. Infectious Diseases (London, England), 48(3), 189-94. https://doi.org/10.3109/23744235.2015.1103894
Yang H, et al. In Vivo Activity of Vancomycin Combined With Colistin Against Multidrug-resistant Strains of Acinetobacter Baumannii in a Galleria Mellonella Model. Infect Dis (Lond). 2016;48(3):189-94. PubMed PMID: 26503239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo activity of vancomycin combined with colistin against multidrug-resistant strains of Acinetobacter baumannii in a Galleria mellonella model. AU - Yang,Haifei, AU - Lv,Na, AU - Hu,Lifen, AU - Liu,Yanyan, AU - Cheng,Jun, AU - Ye,Ying, AU - Li,Jiabin, Y1 - 2015/10/26/ PY - 2015/10/28/entrez PY - 2015/10/28/pubmed PY - 2016/6/23/medline KW - Acinetobacter baumannii KW - Galleria mellonella KW - colistin KW - insect model KW - vancomycin SP - 189 EP - 94 JF - Infectious diseases (London, England) JO - Infect Dis (Lond) VL - 48 IS - 3 N2 - BACKGROUND: With increasing antibiotic resistance, the selection of effective treatment of A. baumannii infections is particularly challenging. METHODS: This study assessed the activities of the combination of vancomycin and colistin combination in vitro and in vivo using a Galleria mellonella model against four colistin-susceptible or colistin-resistant A. baumannii strains. RESULTS: In checkerboard assays, synergy was observed between vancomycin and colistin for all four strains tested (0.156 ≤ Fractional inhibitory concentration indices [FICI] ≤ 0.281). In time-kill assays, the combination showed continued bactericidal activity and synergy after 24 h for colistin-susceptible strains. For colistin-resistant strains, the combination resulted in bactericidal activity within 8 h, but sustained bacterial re-growth was then observed. Treatment of G. mellonella larvae infected with lethal doses of A. baumannii (except 19606R) resulted in significantly increased survival rates when vancomycin was given with colistin compared to colistin treatment alone (p < 0.05). CONCLUSIONS: These findings suggest that regimens containing vancomycin may be useful for infections due to multidrug-resistant Acinetobacter baumannii. SN - 2374-4243 UR - https://www.unboundmedicine.com/medline/citation/26503239/In_vivo_activity_of_vancomycin_combined_with_colistin_against_multidrug_resistant_strains_of_Acinetobacter_baumannii_in_a_Galleria_mellonella_model_ L2 - http://www.tandfonline.com/doi/abs/10.3109/23744235.2015.1103894 DB - PRIME DP - Unbound Medicine ER -