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Mitochondrial superoxide dismutase deficiency accelerates chronological aging in the fission yeast Schizosaccharomyces pombe.
Cell Biol Int. 2016 Jan; 40(1):100-6.CB

Abstract

A mitochondrial superoxide dismutase (SOD2) is the first line of antioxidant defense against mitochondrial superoxide. Even though the involvement of SOD2 in lifespan has been studied extensively in several organisms, characterization of the aging process has not been performed for the sod2 mutant (sod2Δ) of a prominent model Schizosaccharomyces pombe. In this study, we measured the chronological lifespan of sod2Δ cells by their ability to survive in long-term culture. SOD2 deficiency drastically decreased cell viability in the stationary phase. The mutation frequency of nuclear DNA in sod2Δ was elevated in the stationary phase, and cellular proteins and nuclear DNA were extensively degraded, concurrent with cell death. The sod2 gene in wild-type cells could be induced by an increase in endogenous oxidative stresses, after which, SOD2 activity was substantially elevated during the stationary phase. Culture in a lower glucose concentration (calorie restriction) prominently extended the sod2Δ lifespan. Therefore, S. pombe SOD2 plays a critical role in longevity through its upregulation in the non-dividing phase.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama, 700-0005, Japan.Department of Biochemistry, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama, 700-0005, Japan.Department of Biochemistry, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama, 700-0005, Japan.Department of Biochemistry, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama, 700-0005, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26507459

Citation

Ogata, Toshiya, et al. "Mitochondrial Superoxide Dismutase Deficiency Accelerates Chronological Aging in the Fission Yeast Schizosaccharomyces Pombe." Cell Biology International, vol. 40, no. 1, 2016, pp. 100-6.
Ogata T, Senoo T, Kawano S, et al. Mitochondrial superoxide dismutase deficiency accelerates chronological aging in the fission yeast Schizosaccharomyces pombe. Cell Biol Int. 2016;40(1):100-6.
Ogata, T., Senoo, T., Kawano, S., & Ikeda, S. (2016). Mitochondrial superoxide dismutase deficiency accelerates chronological aging in the fission yeast Schizosaccharomyces pombe. Cell Biology International, 40(1), 100-6. https://doi.org/10.1002/cbin.10556
Ogata T, et al. Mitochondrial Superoxide Dismutase Deficiency Accelerates Chronological Aging in the Fission Yeast Schizosaccharomyces Pombe. Cell Biol Int. 2016;40(1):100-6. PubMed PMID: 26507459.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mitochondrial superoxide dismutase deficiency accelerates chronological aging in the fission yeast Schizosaccharomyces pombe. AU - Ogata,Toshiya, AU - Senoo,Takanori, AU - Kawano,Shinji, AU - Ikeda,Shogo, Y1 - 2015/11/19/ PY - 2015/06/09/received PY - 2015/10/23/accepted PY - 2015/10/29/entrez PY - 2015/10/29/pubmed PY - 2016/12/15/medline KW - Schizosaccharomyces pombe KW - calorie restriction KW - chronological lifespan KW - mitochondrial SOD KW - oxidative damage SP - 100 EP - 6 JF - Cell biology international JO - Cell Biol Int VL - 40 IS - 1 N2 - A mitochondrial superoxide dismutase (SOD2) is the first line of antioxidant defense against mitochondrial superoxide. Even though the involvement of SOD2 in lifespan has been studied extensively in several organisms, characterization of the aging process has not been performed for the sod2 mutant (sod2Δ) of a prominent model Schizosaccharomyces pombe. In this study, we measured the chronological lifespan of sod2Δ cells by their ability to survive in long-term culture. SOD2 deficiency drastically decreased cell viability in the stationary phase. The mutation frequency of nuclear DNA in sod2Δ was elevated in the stationary phase, and cellular proteins and nuclear DNA were extensively degraded, concurrent with cell death. The sod2 gene in wild-type cells could be induced by an increase in endogenous oxidative stresses, after which, SOD2 activity was substantially elevated during the stationary phase. Culture in a lower glucose concentration (calorie restriction) prominently extended the sod2Δ lifespan. Therefore, S. pombe SOD2 plays a critical role in longevity through its upregulation in the non-dividing phase. SN - 1095-8355 UR - https://www.unboundmedicine.com/medline/citation/26507459/Mitochondrial_superoxide_dismutase_deficiency_accelerates_chronological_aging_in_the_fission_yeast_Schizosaccharomyces_pombe_ L2 - https://doi.org/10.1002/cbin.10556 DB - PRIME DP - Unbound Medicine ER -