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Sanguiin H6 suppresses TGF-β induction of the epithelial-mesenchymal transition and inhibits migration and invasion in A549 lung cancer.
Bioorg Med Chem Lett. 2015 Dec 01; 25(23):5508-13.BM

Abstract

In the epithelial-mesenchymal transition (EMT), an important cellular process, epithelial cells become mesenchymal cells. This process is also critically involved in cancer metastasis. Sanguiin H6 is a compound derived from ellagitannin, which is found in berries. Sanguiin H6 shows various pharmacological properties, including anti-angiogenic activity. Because the possible role of sanguiin H6 in the EMT and the underlying molecular mechanisms are unclear, we investigated the effect of sanguiin H6 on the EMT. Transforming growth factor-beta 1 (TGF-β1) induces the EMT and promotes lung adenocarcinoma migration and invasion through the Smad2/3 signaling pathway. Thus, to understand the inhibitory effects of sanguiin H6 on lung cancer migration and invasion, we investigated the ability of sanguiin H6 to inhibit TGF-β1-induced EMT in the A549 cell line. We found that sanguiin H6 significantly prevented the activation of Smad2/3 signaling pathway by TGF-β1. Additionally, sanguiin H6 increased the expression of the epithelial marker E-cadherin and repressed the expression of Snail and the mesenchymal marker N-cadherin during TGF-β1-induced EMT. Moreover, sanguiin H6 regulated the expression of EMT-dependent genes induced by TGF-β1. Finally, sanguiin H6 inhibited the migration and invasion of TGF-β1-stimulated A549 cells. Taken together, our findings provide new evidence that sanguiin H6 suppresses lung cancer migration and invasion in vitro by inhibiting TGF-β1 induction of the EMT.

Authors+Show Affiliations

Laboratory of Molecular Oncology, Cheil General Hospital & Women's Healthcare Center, Dankook University College of Medicine, Seoul, South Korea.Department of Biomedical Sciences, University of Ulsan College of Medicine, Seoul, South Korea; Department of Pharmacology, University of Ulsan College of Medicine, Seoul, South Korea; Cell Dysfunction Research Center (CDRC), University of Ulsan College of Medicine, Seoul, South Korea.College of Korean Medicine, Gachon University, Seongnam, South Korea.Division of Nutrition and Metabolism Research Group, Korea Food Research Institute, Gyeonggi-do, South Korea.College of Korean Medicine, Gachon University, Seongnam, South Korea. Electronic address: kkang@gachon.ac.kr.Department of Biomedical Sciences, University of Ulsan College of Medicine, Seoul, South Korea; Department of Pharmacology, University of Ulsan College of Medicine, Seoul, South Korea; Cell Dysfunction Research Center (CDRC), University of Ulsan College of Medicine, Seoul, South Korea. Electronic address: choikc75@amc.seoul.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26508552

Citation

Ko, Hyeonseok, et al. "Sanguiin H6 Suppresses TGF-β Induction of the Epithelial-mesenchymal Transition and Inhibits Migration and Invasion in A549 Lung Cancer." Bioorganic & Medicinal Chemistry Letters, vol. 25, no. 23, 2015, pp. 5508-13.
Ko H, Jeon H, Lee D, et al. Sanguiin H6 suppresses TGF-β induction of the epithelial-mesenchymal transition and inhibits migration and invasion in A549 lung cancer. Bioorg Med Chem Lett. 2015;25(23):5508-13.
Ko, H., Jeon, H., Lee, D., Choi, H. K., Kang, K. S., & Choi, K. C. (2015). Sanguiin H6 suppresses TGF-β induction of the epithelial-mesenchymal transition and inhibits migration and invasion in A549 lung cancer. Bioorganic & Medicinal Chemistry Letters, 25(23), 5508-13. https://doi.org/10.1016/j.bmcl.2015.10.067
Ko H, et al. Sanguiin H6 Suppresses TGF-β Induction of the Epithelial-mesenchymal Transition and Inhibits Migration and Invasion in A549 Lung Cancer. Bioorg Med Chem Lett. 2015 Dec 1;25(23):5508-13. PubMed PMID: 26508552.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sanguiin H6 suppresses TGF-β induction of the epithelial-mesenchymal transition and inhibits migration and invasion in A549 lung cancer. AU - Ko,Hyeonseok, AU - Jeon,Hyelin, AU - Lee,Dahae, AU - Choi,Hyo-Kyoung, AU - Kang,Ki Sung, AU - Choi,Kyung-Chul, Y1 - 2015/10/23/ PY - 2015/09/15/received PY - 2015/10/18/revised PY - 2015/10/21/accepted PY - 2015/10/29/entrez PY - 2015/10/29/pubmed PY - 2016/10/7/medline KW - Epithelial–mesenchymal transition (EMT) KW - Invasion KW - Lung cancer KW - Migration KW - Sanguiin H6 KW - Smad2/3 signaling pathway KW - Transforming growth factor-beta 1 (TGF-β1) SP - 5508 EP - 13 JF - Bioorganic & medicinal chemistry letters JO - Bioorg Med Chem Lett VL - 25 IS - 23 N2 - In the epithelial-mesenchymal transition (EMT), an important cellular process, epithelial cells become mesenchymal cells. This process is also critically involved in cancer metastasis. Sanguiin H6 is a compound derived from ellagitannin, which is found in berries. Sanguiin H6 shows various pharmacological properties, including anti-angiogenic activity. Because the possible role of sanguiin H6 in the EMT and the underlying molecular mechanisms are unclear, we investigated the effect of sanguiin H6 on the EMT. Transforming growth factor-beta 1 (TGF-β1) induces the EMT and promotes lung adenocarcinoma migration and invasion through the Smad2/3 signaling pathway. Thus, to understand the inhibitory effects of sanguiin H6 on lung cancer migration and invasion, we investigated the ability of sanguiin H6 to inhibit TGF-β1-induced EMT in the A549 cell line. We found that sanguiin H6 significantly prevented the activation of Smad2/3 signaling pathway by TGF-β1. Additionally, sanguiin H6 increased the expression of the epithelial marker E-cadherin and repressed the expression of Snail and the mesenchymal marker N-cadherin during TGF-β1-induced EMT. Moreover, sanguiin H6 regulated the expression of EMT-dependent genes induced by TGF-β1. Finally, sanguiin H6 inhibited the migration and invasion of TGF-β1-stimulated A549 cells. Taken together, our findings provide new evidence that sanguiin H6 suppresses lung cancer migration and invasion in vitro by inhibiting TGF-β1 induction of the EMT. SN - 1464-3405 UR - https://www.unboundmedicine.com/medline/citation/26508552/Sanguiin_H6_suppresses_TGF_β_induction_of_the_epithelial_mesenchymal_transition_and_inhibits_migration_and_invasion_in_A549_lung_cancer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-894X(15)30175-X DB - PRIME DP - Unbound Medicine ER -