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Association of vitamin D receptor gene polymorphisms and serum 25-hydroxyvitamin D levels with Crohn's disease in Chinese patients.
J Gastroenterol Hepatol. 2016 Apr; 31(4):795-801.JG

Abstract

BACKGROUND AND AIM

The vitamin D receptor (VDR) regulates immune responses and inflammation through binding with 1,25-dihydroxyvitamin D, the active form of vitamin D. The serum 25-hydroxyvitamin D (25(OH)D) level clinically reflects vitamin D status in the human body. We investigated the association of VDR polymorphisms and 25(OH)D levels in Chinese patients with Crohn's disease (CD).

METHODS

Vitamin D receptor polymorphisms (FokI, BsmI, ApaI, and TaqI) were genotyped by SNaPshot. Serum 25(OH)D levels were measured by electro-chemiluminescence immunoassay.

RESULTS

A total of 297 patients with CD and 446 controls were recruited. Compared with controls, mutant alleles and genotypes of BsmI and TaqI were less prevalent in patients with CD (all P < 0.05/4 = 0.0125). The AAC haplotype formed by BsmI, ApaI, and TaqI was also less prevalent in patients with CD (P = 0.004). Furthermore, 124 patients and 188 controls were randomly selected for measurements of 25(OH)D levels. Average 25(OH)D level was lower in patients with CD than in controls (15.46 ± 8.11 vs 21.64 ± 9.45 ng/mL, P < 0.001) and negatively linked to CD activity index (β = -0.829, P < 0.001), platelet count (β = -0.253, P < 0.001) and neutrophil percentage (β = -0.136, P = 0.005) in patients with CD. The ApaI mutant genotype and vitamin D deficiency (<20 ng/mL) were independently associated with CD (P = 0.009, P < 0.001, respectively). In patients with CD, vitamin D deficiency interacted with FokI, ApaI, and TaqI mutant genotypes (P = 0.027, P = 0.024, and P = 0.040, respectively).

CONCLUSIONS

Vitamin D receptor (BsmI, ApaI, and TaqI) mutations and lower 25(OH)D levels are associated with CD in Chinese patients. Moreover, VDR (FokI, ApaI, and TaqI) mutations and vitamin D deficiency may have a combined impact on CD.

Authors+Show Affiliations

Department of Gastroenterology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.Department of Gastroenterology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.Department of Gastroenterology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.Department of Gastroenterology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.Department of Gastroenterology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.Department of Gastroenterology, The Wenzhou Central Hospital, Wenzhou, China.Department of Gastroenterology, The Wenzhou Central Hospital, Wenzhou, China.Department of Gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.Department of Gastroenterology, The Wenzhou Renmin Hospital, Wenzhou, China.Department of Gastroenterology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26513524

Citation

Xia, Sheng-long, et al. "Association of Vitamin D Receptor Gene Polymorphisms and Serum 25-hydroxyvitamin D Levels With Crohn's Disease in Chinese Patients." Journal of Gastroenterology and Hepatology, vol. 31, no. 4, 2016, pp. 795-801.
Xia SL, Lin XX, Guo MD, et al. Association of vitamin D receptor gene polymorphisms and serum 25-hydroxyvitamin D levels with Crohn's disease in Chinese patients. J Gastroenterol Hepatol. 2016;31(4):795-801.
Xia, S. L., Lin, X. X., Guo, M. D., Zhang, D. G., Zheng, S. Z., Jiang, L. J., Jin, J., Lin, X. Q., Ding, R., & Jiang, Y. (2016). Association of vitamin D receptor gene polymorphisms and serum 25-hydroxyvitamin D levels with Crohn's disease in Chinese patients. Journal of Gastroenterology and Hepatology, 31(4), 795-801. https://doi.org/10.1111/jgh.13221
Xia SL, et al. Association of Vitamin D Receptor Gene Polymorphisms and Serum 25-hydroxyvitamin D Levels With Crohn's Disease in Chinese Patients. J Gastroenterol Hepatol. 2016;31(4):795-801. PubMed PMID: 26513524.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of vitamin D receptor gene polymorphisms and serum 25-hydroxyvitamin D levels with Crohn's disease in Chinese patients. AU - Xia,Sheng-long, AU - Lin,Xin-xin, AU - Guo,Mao-dong, AU - Zhang,Da-guan, AU - Zheng,Shu-zi, AU - Jiang,Li-jia, AU - Jin,Jie, AU - Lin,Xiu-qing, AU - Ding,Ran, AU - Jiang,Yi, PY - 2015/02/04/received PY - 2015/10/13/revised PY - 2015/10/16/accepted PY - 2015/10/30/entrez PY - 2015/10/30/pubmed PY - 2016/12/16/medline KW - 25-hydroxyvitamin D KW - Crohn's disease KW - polymorphism KW - vitamin D receptor SP - 795 EP - 801 JF - Journal of gastroenterology and hepatology JO - J Gastroenterol Hepatol VL - 31 IS - 4 N2 - BACKGROUND AND AIM: The vitamin D receptor (VDR) regulates immune responses and inflammation through binding with 1,25-dihydroxyvitamin D, the active form of vitamin D. The serum 25-hydroxyvitamin D (25(OH)D) level clinically reflects vitamin D status in the human body. We investigated the association of VDR polymorphisms and 25(OH)D levels in Chinese patients with Crohn's disease (CD). METHODS: Vitamin D receptor polymorphisms (FokI, BsmI, ApaI, and TaqI) were genotyped by SNaPshot. Serum 25(OH)D levels were measured by electro-chemiluminescence immunoassay. RESULTS: A total of 297 patients with CD and 446 controls were recruited. Compared with controls, mutant alleles and genotypes of BsmI and TaqI were less prevalent in patients with CD (all P < 0.05/4 = 0.0125). The AAC haplotype formed by BsmI, ApaI, and TaqI was also less prevalent in patients with CD (P = 0.004). Furthermore, 124 patients and 188 controls were randomly selected for measurements of 25(OH)D levels. Average 25(OH)D level was lower in patients with CD than in controls (15.46 ± 8.11 vs 21.64 ± 9.45 ng/mL, P < 0.001) and negatively linked to CD activity index (β = -0.829, P < 0.001), platelet count (β = -0.253, P < 0.001) and neutrophil percentage (β = -0.136, P = 0.005) in patients with CD. The ApaI mutant genotype and vitamin D deficiency (<20 ng/mL) were independently associated with CD (P = 0.009, P < 0.001, respectively). In patients with CD, vitamin D deficiency interacted with FokI, ApaI, and TaqI mutant genotypes (P = 0.027, P = 0.024, and P = 0.040, respectively). CONCLUSIONS: Vitamin D receptor (BsmI, ApaI, and TaqI) mutations and lower 25(OH)D levels are associated with CD in Chinese patients. Moreover, VDR (FokI, ApaI, and TaqI) mutations and vitamin D deficiency may have a combined impact on CD. SN - 1440-1746 UR - https://www.unboundmedicine.com/medline/citation/26513524/Association_of_vitamin_D_receptor_gene_polymorphisms_and_serum_25_hydroxyvitamin_D_levels_with_Crohn's_disease_in_Chinese_patients_ L2 - https://doi.org/10.1111/jgh.13221 DB - PRIME DP - Unbound Medicine ER -