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Is there a role for neurotrophic factors and their receptors in augmenting the neuroprotective effect of (-)-epigallocatechin-3-gallate treatment of sciatic nerve crush injury?
Neuropharmacology 2016; 102:1-20N

Abstract

This study analyzed and compared the effects of EGCG treatment on the expression of NTFs and NTF receptors expression in the sciatic nerve and the L3-L6 spinal cord segments at the early phase of regeneration following sciatic nerve crush injury. Analysis of BDNF, GDNF and NT3 neurotropic factors and Trk-B, Trk-C and NGFR-p75 receptors in neurons in the spinal cord of CRUSH and CRUSH + EGGC rats showed significant (p < 0.0001) decrease compared to NAÏVE and SHAM at day 1, 3, 7 and 14 after nerve injury. EGCG treatment significantly (p < 0.0001) increased the BDNF, GDN, NT3, Trk-B, Trk-C and NGFR-p75 immunostaining in the L3-L6 spinal cord compared to CRUSH animals. Also, EGCG treatment significantly increased the Trk-B protein concentration and Trk-B, NT3 and Trk-C gene expression in the spinal cords compared to CRUSH group. However, at day 1 and 3 post nerve injury, EGCG treatment significantly decreased the NGFR-p75 expression compared to CRUSH rats. In the sciatic nerve, EGCG treatment significantly (p < 0.01) increased the Trk-B and NGFR-p75 protein concentration in the controls. EGCG treatment significantly (p < 0.0001) increased the Trk-B, Trk-C and NGFR-p75 mRNA gene expressions in the sciatic nerves compared to CRUSH group. Only at day 1, CRUSH + EGCG animals displayed significant rise in the sciatic nerves NT3 gene expression compared to CRUSH group. Our data suggest that the EGCG neuroprotective effect on the spinal cord neurons may be mediated through the modulation of NTFs and NTF receptors following nerve crush injury in a rat model.

Authors+Show Affiliations

Department of Anatomy, Faculty of Medicine, Kuwait University, Kuwait. Electronic address: wrenno@hsc.edu.kw.Department of Anatomy, Faculty of Medicine, Kuwait University, Kuwait.Department of Biochemistry, Faculty of Medicine, Kuwait University, Kuwait.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26514400

Citation

Renno, Waleed M., et al. "Is There a Role for Neurotrophic Factors and Their Receptors in Augmenting the Neuroprotective Effect of (-)-epigallocatechin-3-gallate Treatment of Sciatic Nerve Crush Injury?" Neuropharmacology, vol. 102, 2016, pp. 1-20.
Renno WM, Khan KM, Benov L. Is there a role for neurotrophic factors and their receptors in augmenting the neuroprotective effect of (-)-epigallocatechin-3-gallate treatment of sciatic nerve crush injury? Neuropharmacology. 2016;102:1-20.
Renno, W. M., Khan, K. M., & Benov, L. (2016). Is there a role for neurotrophic factors and their receptors in augmenting the neuroprotective effect of (-)-epigallocatechin-3-gallate treatment of sciatic nerve crush injury? Neuropharmacology, 102, pp. 1-20. doi:10.1016/j.neuropharm.2015.10.029.
Renno WM, Khan KM, Benov L. Is There a Role for Neurotrophic Factors and Their Receptors in Augmenting the Neuroprotective Effect of (-)-epigallocatechin-3-gallate Treatment of Sciatic Nerve Crush Injury. Neuropharmacology. 2016;102:1-20. PubMed PMID: 26514400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Is there a role for neurotrophic factors and their receptors in augmenting the neuroprotective effect of (-)-epigallocatechin-3-gallate treatment of sciatic nerve crush injury? AU - Renno,Waleed M, AU - Khan,Khalid M, AU - Benov,Ludmil, Y1 - 2015/10/26/ PY - 2015/06/09/received PY - 2015/10/01/revised PY - 2015/10/23/accepted PY - 2015/10/31/entrez PY - 2015/10/31/pubmed PY - 2016/10/8/medline KW - BDGF KW - GDGF KW - NGFR-p75 KW - NT-3 KW - Neuroregeneration KW - Trk-B SP - 1 EP - 20 JF - Neuropharmacology JO - Neuropharmacology VL - 102 N2 - This study analyzed and compared the effects of EGCG treatment on the expression of NTFs and NTF receptors expression in the sciatic nerve and the L3-L6 spinal cord segments at the early phase of regeneration following sciatic nerve crush injury. Analysis of BDNF, GDNF and NT3 neurotropic factors and Trk-B, Trk-C and NGFR-p75 receptors in neurons in the spinal cord of CRUSH and CRUSH + EGGC rats showed significant (p < 0.0001) decrease compared to NAÏVE and SHAM at day 1, 3, 7 and 14 after nerve injury. EGCG treatment significantly (p < 0.0001) increased the BDNF, GDN, NT3, Trk-B, Trk-C and NGFR-p75 immunostaining in the L3-L6 spinal cord compared to CRUSH animals. Also, EGCG treatment significantly increased the Trk-B protein concentration and Trk-B, NT3 and Trk-C gene expression in the spinal cords compared to CRUSH group. However, at day 1 and 3 post nerve injury, EGCG treatment significantly decreased the NGFR-p75 expression compared to CRUSH rats. In the sciatic nerve, EGCG treatment significantly (p < 0.01) increased the Trk-B and NGFR-p75 protein concentration in the controls. EGCG treatment significantly (p < 0.0001) increased the Trk-B, Trk-C and NGFR-p75 mRNA gene expressions in the sciatic nerves compared to CRUSH group. Only at day 1, CRUSH + EGCG animals displayed significant rise in the sciatic nerves NT3 gene expression compared to CRUSH group. Our data suggest that the EGCG neuroprotective effect on the spinal cord neurons may be mediated through the modulation of NTFs and NTF receptors following nerve crush injury in a rat model. SN - 1873-7064 UR - https://www.unboundmedicine.com/medline/citation/26514400/Is_there_a_role_for_neurotrophic_factors_and_their_receptors_in_augmenting_the_neuroprotective_effect_of_____epigallocatechin_3_gallate_treatment_of_sciatic_nerve_crush_injury L2 - https://linkinghub.elsevier.com/retrieve/pii/S0028-3908(15)30152-0 DB - PRIME DP - Unbound Medicine ER -