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Evaluation of an enzyme immunoassay for antibodies to a recombinant Blastomyces adhesin-1 repeat antigen as an aid in the diagnosis of blastomycosis in dogs.
J Am Vet Med Assoc. 2015 Nov 15; 247(10):1133-8.JA

Abstract

OBJECTIVE

To evaluate the sensitivity and specificity of an enzyme immunoassay (EIA) for antibodies to a recombinant Blastomyces adhesin-1 repeat antigen (rBAD-1) to aid in the diagnosis of blastomycosis in dogs and compare the findings with results from other tests used for this purpose.

DESIGN

Prospective analytic study.

SAMPLE

Serum and urine from 70 dogs with and without blastomycosis.

PROCEDURES

Serum and urine samples were collected from dogs with blastomycosis (n = 21), histoplasmosis (8), or nonfungal pulmonary disease (21) and from healthy control dogs living in a blastomycosis-endemic area (20). Serum was tested for antibodies against Blastomyces dermatitidis with the rBAD-1 antibody EIA and an A-antigen antibody agar gel immunodiffusion (AGID) assay. Serum and urine were tested for B dermatitidis antigen with a quantitative EIA.

RESULTS

Sensitivity of the quantitative antigen EIA was 100% in serum and urine samples from dogs with blastomycosis, with specificity of 95% in urine samples from dogs with nonfungal pulmonary disease and 100% in urine samples from healthy dogs. Sensitivity of the rBAD-1 antibody EIA (95%) was significantly greater than that of the A-antigen antibody AGID assay (65%). Specificity of the antibody EIA was 88% in dogs with histoplasmosis, 95% in healthy dogs, and 100% in dogs with nonfungal pulmonary disease.

CONCLUSIONS AND CLINICAL RELEVANCE

The rBAD-1 antibody EIA had greater sensitivity than the A-antigen antibody AGID assay in dogs with blastomycosis. This antibody EIA may assist in distinguishing histoplasmosis from blastomycosis. Further evaluation in a larger prospective study is needed to verify these results.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26517616

Citation

Mourning, Alyssa C., et al. "Evaluation of an Enzyme Immunoassay for Antibodies to a Recombinant Blastomyces Adhesin-1 Repeat Antigen as an Aid in the Diagnosis of Blastomycosis in Dogs." Journal of the American Veterinary Medical Association, vol. 247, no. 10, 2015, pp. 1133-8.
Mourning AC, Patterson EE, Kirsch EJ, et al. Evaluation of an enzyme immunoassay for antibodies to a recombinant Blastomyces adhesin-1 repeat antigen as an aid in the diagnosis of blastomycosis in dogs. J Am Vet Med Assoc. 2015;247(10):1133-8.
Mourning, A. C., Patterson, E. E., Kirsch, E. J., Renschler, J. S., Wolf, L. A., Paris, J. K., Durkin, M. M., & Wheat, L. J. (2015). Evaluation of an enzyme immunoassay for antibodies to a recombinant Blastomyces adhesin-1 repeat antigen as an aid in the diagnosis of blastomycosis in dogs. Journal of the American Veterinary Medical Association, 247(10), 1133-8. https://doi.org/10.2460/javma.247.10.1133
Mourning AC, et al. Evaluation of an Enzyme Immunoassay for Antibodies to a Recombinant Blastomyces Adhesin-1 Repeat Antigen as an Aid in the Diagnosis of Blastomycosis in Dogs. J Am Vet Med Assoc. 2015 Nov 15;247(10):1133-8. PubMed PMID: 26517616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of an enzyme immunoassay for antibodies to a recombinant Blastomyces adhesin-1 repeat antigen as an aid in the diagnosis of blastomycosis in dogs. AU - Mourning,Alyssa C, AU - Patterson,Edward E, AU - Kirsch,Emily J, AU - Renschler,Janelle S, AU - Wolf,Linda A, AU - Paris,Jasmin K, AU - Durkin,Michelle M, AU - Wheat,Lawrence J, PY - 2015/10/31/entrez PY - 2015/11/1/pubmed PY - 2016/9/13/medline SP - 1133 EP - 8 JF - Journal of the American Veterinary Medical Association JO - J Am Vet Med Assoc VL - 247 IS - 10 N2 - OBJECTIVE: To evaluate the sensitivity and specificity of an enzyme immunoassay (EIA) for antibodies to a recombinant Blastomyces adhesin-1 repeat antigen (rBAD-1) to aid in the diagnosis of blastomycosis in dogs and compare the findings with results from other tests used for this purpose. DESIGN: Prospective analytic study. SAMPLE: Serum and urine from 70 dogs with and without blastomycosis. PROCEDURES: Serum and urine samples were collected from dogs with blastomycosis (n = 21), histoplasmosis (8), or nonfungal pulmonary disease (21) and from healthy control dogs living in a blastomycosis-endemic area (20). Serum was tested for antibodies against Blastomyces dermatitidis with the rBAD-1 antibody EIA and an A-antigen antibody agar gel immunodiffusion (AGID) assay. Serum and urine were tested for B dermatitidis antigen with a quantitative EIA. RESULTS: Sensitivity of the quantitative antigen EIA was 100% in serum and urine samples from dogs with blastomycosis, with specificity of 95% in urine samples from dogs with nonfungal pulmonary disease and 100% in urine samples from healthy dogs. Sensitivity of the rBAD-1 antibody EIA (95%) was significantly greater than that of the A-antigen antibody AGID assay (65%). Specificity of the antibody EIA was 88% in dogs with histoplasmosis, 95% in healthy dogs, and 100% in dogs with nonfungal pulmonary disease. CONCLUSIONS AND CLINICAL RELEVANCE: The rBAD-1 antibody EIA had greater sensitivity than the A-antigen antibody AGID assay in dogs with blastomycosis. This antibody EIA may assist in distinguishing histoplasmosis from blastomycosis. Further evaluation in a larger prospective study is needed to verify these results. SN - 1943-569X UR - https://www.unboundmedicine.com/medline/citation/26517616/Evaluation_of_an_enzyme_immunoassay_for_antibodies_to_a_recombinant_Blastomyces_adhesin_1_repeat_antigen_as_an_aid_in_the_diagnosis_of_blastomycosis_in_dogs_ L2 - https://avmajournals.avma.org/doi/10.2460/javma.247.10.1133?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -