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Carnosine and taurine treatments diminished brain oxidative stress and apoptosis in D-galactose aging model.
Metab Brain Dis 2016; 31(2):337-45MB

Abstract

D-galactose (GAL) has been used as an animal model for brain aging and antiaging studies. GAL stimulates oxidative stress in several tissues including brain. Carnosine (CAR; β-alanil-L-histidine) and taurine (TAU; 2-aminoethanesulfonic acid) exhibit antioxidant properties. CAR and TAU have anti-aging and neuroprotective effects. We investigated the effect of CAR and TAU supplementations on oxidative stress and brain damage in GAL-treated rats. Rats received GAL (300 mg/kg; s.c.; 5 days per week) alone or together with CAR (250 mg/kg/daily; i.p.; 5 days per week) or TAU (2.5% w/w; in rat chow) for 2 months. Brain malondialdehyde (MDA), protein carbonyl (PC) and glutathione (GSH) levels and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione transferase (GST) and acetylcholinesterase (AChE) activities were determined. Expressions of B cell lymphoma-2 (Bcl-2), Bax and caspase-3 were also evaluated in the brains by immunohistochemistry. GAL treatment increased brain MDA and PC levels and AChE activities. It decreased significantly brain GSH levels, SOD and GSH-Px but not GST activities. GAL treatment caused histopathological changes and increased apoptosis. CAR and TAU significantly reduced brain AChE activities, MDA and PC levels and elevated GSH levels in GAL-treated rats. CAR, but not TAU, significantly increased low activities of SOD and GSH-Px. Both CAR and TAU diminished apoptosis and ameliorated histopathological findings in the brain of GAL-treated rats. Our results indicate that CAR and TAU may be effective to prevent the development of oxidative stress, apoptosis and histopathological deterioration in the brain of GAL-treated rats.

Authors+Show Affiliations

Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.Department of Biochemistry, Yeditepe University Medical Faculty, Istanbul, Turkey.Department of Pathology, Yeditepe University Medical Faculty, Istanbul, Turkey.Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey. m.mujdatuysal@hotmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26518192

Citation

Aydın, A Fatih, et al. "Carnosine and Taurine Treatments Diminished Brain Oxidative Stress and Apoptosis in D-galactose Aging Model." Metabolic Brain Disease, vol. 31, no. 2, 2016, pp. 337-45.
Aydın AF, Çoban J, Doğan-Ekici I, et al. Carnosine and taurine treatments diminished brain oxidative stress and apoptosis in D-galactose aging model. Metab Brain Dis. 2016;31(2):337-45.
Aydın, A. F., Çoban, J., Doğan-Ekici, I., Betül-Kalaz, E., Doğru-Abbasoğlu, S., & Uysal, M. (2016). Carnosine and taurine treatments diminished brain oxidative stress and apoptosis in D-galactose aging model. Metabolic Brain Disease, 31(2), pp. 337-45. doi:10.1007/s11011-015-9755-0.
Aydın AF, et al. Carnosine and Taurine Treatments Diminished Brain Oxidative Stress and Apoptosis in D-galactose Aging Model. Metab Brain Dis. 2016;31(2):337-45. PubMed PMID: 26518192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carnosine and taurine treatments diminished brain oxidative stress and apoptosis in D-galactose aging model. AU - Aydın,A Fatih, AU - Çoban,Jale, AU - Doğan-Ekici,Işın, AU - Betül-Kalaz,Esra, AU - Doğru-Abbasoğlu,Semra, AU - Uysal,Müjdat, Y1 - 2015/10/31/ PY - 2015/05/11/received PY - 2015/10/25/accepted PY - 2015/11/1/entrez PY - 2015/11/1/pubmed PY - 2017/1/11/medline KW - Brain KW - Carnosine KW - D-Galactose KW - Oxidative stress KW - Taurine SP - 337 EP - 45 JF - Metabolic brain disease JO - Metab Brain Dis VL - 31 IS - 2 N2 - D-galactose (GAL) has been used as an animal model for brain aging and antiaging studies. GAL stimulates oxidative stress in several tissues including brain. Carnosine (CAR; β-alanil-L-histidine) and taurine (TAU; 2-aminoethanesulfonic acid) exhibit antioxidant properties. CAR and TAU have anti-aging and neuroprotective effects. We investigated the effect of CAR and TAU supplementations on oxidative stress and brain damage in GAL-treated rats. Rats received GAL (300 mg/kg; s.c.; 5 days per week) alone or together with CAR (250 mg/kg/daily; i.p.; 5 days per week) or TAU (2.5% w/w; in rat chow) for 2 months. Brain malondialdehyde (MDA), protein carbonyl (PC) and glutathione (GSH) levels and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione transferase (GST) and acetylcholinesterase (AChE) activities were determined. Expressions of B cell lymphoma-2 (Bcl-2), Bax and caspase-3 were also evaluated in the brains by immunohistochemistry. GAL treatment increased brain MDA and PC levels and AChE activities. It decreased significantly brain GSH levels, SOD and GSH-Px but not GST activities. GAL treatment caused histopathological changes and increased apoptosis. CAR and TAU significantly reduced brain AChE activities, MDA and PC levels and elevated GSH levels in GAL-treated rats. CAR, but not TAU, significantly increased low activities of SOD and GSH-Px. Both CAR and TAU diminished apoptosis and ameliorated histopathological findings in the brain of GAL-treated rats. Our results indicate that CAR and TAU may be effective to prevent the development of oxidative stress, apoptosis and histopathological deterioration in the brain of GAL-treated rats. SN - 1573-7365 UR - https://www.unboundmedicine.com/medline/citation/26518192/Carnosine_and_taurine_treatments_diminished_brain_oxidative_stress_and_apoptosis_in_D_galactose_aging_model_ L2 - https://doi.org/10.1007/s11011-015-9755-0 DB - PRIME DP - Unbound Medicine ER -