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Identification and mapping of Tril, a homeodomain-leucine zipper gene involved in multicellular trichome initiation in Cucumis sativus.
Theor Appl Genet. 2016 Feb; 129(2):305-16.TA

Abstract

KEY MESSAGE

Using map-based cloning of Tril gene, we identified a homeodomain-leucine zipper gene involved in the initiation of multicellular trichomes (including the spines of fruit) in cucumber.

ABSTRACT

Fruit spines are a special type of trichome that impacts the quality and appearance of cucumber (Cucumis sativus L.) fruit. Scanning electron microscopy revealed that the trichome-less (tril) mutant originating from European greenhouse cucumber has a completely glabrous phenotype on cotyledons, hypocotyls, young leaves, fruits, and fruit stalks. Genetic analysis revealed that tril was inherited as a recessive allele at a single locus. Using 1058 F2 individuals derived from a cross between cucumber tril mutant CGN19839 and the micro-trichome (mict) mutant 06-2, tril was mapped to chromosome 6, and narrowed down to a 37.4 kb genomic region which carries seven predicted genes. Genetic and molecular analyses revealed that gene Cucsa.045360 is a possible candidate gene for the differentiation of epidermal cells to trichomes. It is a member of the class IV homeodomain-leucine zipper (HD-Zip IV) family and encodes homeodomain and START domain, sharing 66.7% predicted amino acid sequence identity to PROTODERMAL FACTOR2 (PDF2) and 35.0% to GLABRA2 (GL2) of Arabidopsis. The homeobox domain had changed amino acid sequence because of an insertion in tril mutant. The results of genetic analysis and transcriptome profiling indicated that the Tril gene had an epistatic effect on the Mict gene in trichome development. Phenotypes of the tril mutant such as glabrous fruits and female flowers at every node could be used in developing new cultivars.

Authors+Show Affiliations

School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.Hunan Vegetable Research Institute, Hunan Academy of Agriculture Sciences, Changsha, 410125, China.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China. jspan71@sjtu.edu.cn.School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai, 200240, China. cairun@sjtu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26518574

Citation

Wang, Yun-Li, et al. "Identification and Mapping of Tril, a Homeodomain-leucine Zipper Gene Involved in Multicellular Trichome Initiation in Cucumis Sativus." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 129, no. 2, 2016, pp. 305-16.
Wang YL, Nie JT, Chen HM, et al. Identification and mapping of Tril, a homeodomain-leucine zipper gene involved in multicellular trichome initiation in Cucumis sativus. Theor Appl Genet. 2016;129(2):305-16.
Wang, Y. L., Nie, J. T., Chen, H. M., Guo, C. L., Pan, J., He, H. L., Pan, J. S., & Cai, R. (2016). Identification and mapping of Tril, a homeodomain-leucine zipper gene involved in multicellular trichome initiation in Cucumis sativus. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 129(2), 305-16. https://doi.org/10.1007/s00122-015-2628-4
Wang YL, et al. Identification and Mapping of Tril, a Homeodomain-leucine Zipper Gene Involved in Multicellular Trichome Initiation in Cucumis Sativus. Theor Appl Genet. 2016;129(2):305-16. PubMed PMID: 26518574.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and mapping of Tril, a homeodomain-leucine zipper gene involved in multicellular trichome initiation in Cucumis sativus. AU - Wang,Yun-Li, AU - Nie,Jing-tao, AU - Chen,Hui-Ming, AU - Guo,Chun-li, AU - Pan,Jian, AU - He,Huan-Le, AU - Pan,Jun-Song, AU - Cai,Run, Y1 - 2015/10/30/ PY - 2015/07/08/received PY - 2015/10/17/accepted PY - 2015/11/1/entrez PY - 2015/11/1/pubmed PY - 2016/6/9/medline SP - 305 EP - 16 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 129 IS - 2 N2 - KEY MESSAGE: Using map-based cloning of Tril gene, we identified a homeodomain-leucine zipper gene involved in the initiation of multicellular trichomes (including the spines of fruit) in cucumber. ABSTRACT: Fruit spines are a special type of trichome that impacts the quality and appearance of cucumber (Cucumis sativus L.) fruit. Scanning electron microscopy revealed that the trichome-less (tril) mutant originating from European greenhouse cucumber has a completely glabrous phenotype on cotyledons, hypocotyls, young leaves, fruits, and fruit stalks. Genetic analysis revealed that tril was inherited as a recessive allele at a single locus. Using 1058 F2 individuals derived from a cross between cucumber tril mutant CGN19839 and the micro-trichome (mict) mutant 06-2, tril was mapped to chromosome 6, and narrowed down to a 37.4 kb genomic region which carries seven predicted genes. Genetic and molecular analyses revealed that gene Cucsa.045360 is a possible candidate gene for the differentiation of epidermal cells to trichomes. It is a member of the class IV homeodomain-leucine zipper (HD-Zip IV) family and encodes homeodomain and START domain, sharing 66.7% predicted amino acid sequence identity to PROTODERMAL FACTOR2 (PDF2) and 35.0% to GLABRA2 (GL2) of Arabidopsis. The homeobox domain had changed amino acid sequence because of an insertion in tril mutant. The results of genetic analysis and transcriptome profiling indicated that the Tril gene had an epistatic effect on the Mict gene in trichome development. Phenotypes of the tril mutant such as glabrous fruits and female flowers at every node could be used in developing new cultivars. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/26518574/Identification_and_mapping_of_Tril_a_homeodomain_leucine_zipper_gene_involved_in_multicellular_trichome_initiation_in_Cucumis_sativus_ L2 - https://dx.doi.org/10.1007/s00122-015-2628-4 DB - PRIME DP - Unbound Medicine ER -