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Stability, purification, and applications of bromelain: A review.
Biotechnol Prog 2016 Jan-Feb; 32(1):5-13BP

Abstract

Bromelain is a cysteine protease found in pineapple tissue. Because of its anti-inflammatory and anti-cancer activities, as well as its ability to induce apoptotic cell death, bromelain has proved useful in several therapeutic areas. The market for this protease is growing, and several studies exploring various properties of this molecule have been reported. This review aims to compile this data, and summarize the main findings on bromelain in the literature to date. The physicochemical properties and stability of bromelain under different conditions are discussed. Several studies on the purification of bromelain from crude extracts using a wide range of techniques such as liquid-liquid extractions by aqueous two-phase system, ultrafiltration, precipitation, and chromatography, have been reported. Finally, the various applications of bromelain are presented. This review therefore covers the main properties of bromelain, aiming to provide an up-to-date compilation of the data reported on this enzyme.

Authors+Show Affiliations

Dept. of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil. Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), University of Campinas, Campinas, Brazil.Dept. of Pharmacy, School of Pharmaceutical Science, Sorocaba University, Sorocaba, Brazil.Dept. of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.Dept. of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, UNESP-University Estadual Paulista, Araraquara, Brazil.Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), University of Campinas, Campinas, Brazil.Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), University of Campinas, Campinas, Brazil.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

26518672

Citation

de Lencastre Novaes, Letícia Celia, et al. "Stability, Purification, and Applications of Bromelain: a Review." Biotechnology Progress, vol. 32, no. 1, 2016, pp. 5-13.
de Lencastre Novaes LC, Jozala AF, Lopes AM, et al. Stability, purification, and applications of bromelain: A review. Biotechnol Prog. 2016;32(1):5-13.
de Lencastre Novaes, L. C., Jozala, A. F., Lopes, A. M., de Carvalho Santos-Ebinuma, V., Mazzola, P. G., & Pessoa Junior, A. (2016). Stability, purification, and applications of bromelain: A review. Biotechnology Progress, 32(1), pp. 5-13. doi:10.1002/btpr.2190.
de Lencastre Novaes LC, et al. Stability, Purification, and Applications of Bromelain: a Review. Biotechnol Prog. 2016;32(1):5-13. PubMed PMID: 26518672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stability, purification, and applications of bromelain: A review. AU - de Lencastre Novaes,Letícia Celia, AU - Jozala,Angela Faustino, AU - Lopes,André Moreni, AU - de Carvalho Santos-Ebinuma,Valéria, AU - Mazzola,Priscila Gava, AU - Pessoa Junior,Adalberto, Y1 - 2015/11/17/ PY - 2014/11/13/received PY - 2015/07/24/revised PY - 2015/11/1/entrez PY - 2015/11/1/pubmed PY - 2016/11/12/medline KW - anti-inflammatory KW - bromelain KW - downstream processes KW - pineapple KW - protease KW - purification SP - 5 EP - 13 JF - Biotechnology progress JO - Biotechnol. Prog. VL - 32 IS - 1 N2 - Bromelain is a cysteine protease found in pineapple tissue. Because of its anti-inflammatory and anti-cancer activities, as well as its ability to induce apoptotic cell death, bromelain has proved useful in several therapeutic areas. The market for this protease is growing, and several studies exploring various properties of this molecule have been reported. This review aims to compile this data, and summarize the main findings on bromelain in the literature to date. The physicochemical properties and stability of bromelain under different conditions are discussed. Several studies on the purification of bromelain from crude extracts using a wide range of techniques such as liquid-liquid extractions by aqueous two-phase system, ultrafiltration, precipitation, and chromatography, have been reported. Finally, the various applications of bromelain are presented. This review therefore covers the main properties of bromelain, aiming to provide an up-to-date compilation of the data reported on this enzyme. SN - 1520-6033 UR - https://www.unboundmedicine.com/medline/citation/26518672/Stability_purification_and_applications_of_bromelain:_A_review_ L2 - https://doi.org/10.1002/btpr.2190 DB - PRIME DP - Unbound Medicine ER -