Enhancement of α-linolenic acid content in transgenic tobacco seeds by targeting a plastidial ω-3 fatty acid desaturase (fad7) gene of Sesamum indicum to ER.
Plant Cell Rep. 2016 Jan; 35(1):213-26.PC

Abstract

KEY MESSAGE

Expression of sesame plastidial FAD7 desaturase modified with the endoplasmic reticulum targeting and retention signals, enhances the α-linolenic acid accumulation in seeds of Nicotiana tabacum. In plants, plastidial ω-3 fatty acid desaturase-7 (FAD7) catalyzes the formation of C16 and C18 trienoic fatty acids using organellar glycerolipids and participate in the membrane lipid formation. The plastidial ω-3 desaturases (FAD7) share high sequence homology with the microsomal ω-3 desaturases (FAD3) at the amino acid level except the N-terminal organelle transit peptide. In the present study, the predicted N-terminal plastidial signal peptide of fad7 gene was replaced by the endoplasmic reticulum signal peptide and an endoplasmic reticulum retention signal was placed at the C-terminal. The expression of the modified sesame ω-3 desaturase increases the α-linolenic acid content in the range of 4.78-6.77 % in the seeds of transgenic tobacco plants with concomitant decrease in linoleic acid content. The results suggested the potential of the engineered plastidial ω-3 desaturase from sesame to influence the profile of α-linolenic acid in tobacco plant by shifting the carbon flux from linoleic acid, and thus it can be used in suitable genetic engineering strategy to increase the α-linolenic acid content in sesame and other vegetable oils.

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Authors+Show Affiliations

Bhunia RK

Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, Kharagpur, 721302, India. Advanced Technology Development Centre, Indian Institute of Technology, Kharagpur, 721302, India. Department of Biochemistry, Biophysics and Molecular Biology (BBMB), Iowa State University, Ames, IA, 50011, USA.

Chakraborty A

Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, Kharagpur, 721302, India.

Kaur R

Maiti MK

Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, Kharagpur, 721302, India. Advanced Technology Development Centre, Indian Institute of Technology, Kharagpur, 721302, India. Department of Biotechnology, Indian Institute of Technology, Kharagpur, 721302, India.

Sen SK

Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology, Kharagpur, 721302, India. rupam.micro@gmail.com. Advanced Technology Development Centre, Indian Institute of Technology, Kharagpur, 721302, India. rupam.micro@gmail.com.

MeSH

Amino Acid SequenceComputational BiologyEndoplasmic ReticulumFatty Acid DesaturasesFatty AcidsGene Expression Regulation, PlantPlant ProteinsPlants, Genetically ModifiedPlastidsSeedsSequence AlignmentSequence Analysis, DNASequence DeletionSesamumTobaccoalpha-Linolenic Acid

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26521211

Citation

Bhunia, Rupam Kumar, et al. "Enhancement of Α-linolenic Acid Content in Transgenic Tobacco Seeds By Targeting a Plastidial Ω-3 Fatty Acid Desaturase (fad7) Gene of Sesamum Indicum to ER." Plant Cell Reports, vol. 35, no. 1, 2016, pp. 213-26.

Bhunia RK, Chakraborty A, Kaur R, et al. Enhancement of α-linolenic acid content in transgenic tobacco seeds by targeting a plastidial ω-3 fatty acid desaturase (fad7) gene of Sesamum indicum to ER. Plant Cell Rep. 2016;35(1):213-26.

Bhunia, R. K., Chakraborty, A., Kaur, R., Maiti, M. K., & Sen, S. K. (2016). Enhancement of α-linolenic acid content in transgenic tobacco seeds by targeting a plastidial ω-3 fatty acid desaturase (fad7) gene of Sesamum indicum to ER. Plant Cell Reports, 35(1), 213-26. https://doi.org/10.1007/s00299-015-1880-z

Bhunia RK, et al. Enhancement of Α-linolenic Acid Content in Transgenic Tobacco Seeds By Targeting a Plastidial Ω-3 Fatty Acid Desaturase (fad7) Gene of Sesamum Indicum to ER. Plant Cell Rep. 2016;35(1):213-26. PubMed PMID: 26521211.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Enhancement of α-linolenic acid content in transgenic tobacco seeds by targeting a plastidial ω-3 fatty acid desaturase (fad7) gene of Sesamum indicum to ER. AU - Bhunia,Rupam Kumar, AU - Chakraborty,Anirban, AU - Kaur,Ranjeet, AU - Maiti,Mrinal K, AU - Sen,Soumitra Kumar, Y1 - 2015/10/31/ PY - 2015/06/24/received PY - 2015/10/07/accepted PY - 2015/09/25/revised PY - 2015/11/2/entrez PY - 2015/11/2/pubmed PY - 2016/12/15/medline KW - Endoplasmic reticulum KW - Nicotiana tabacum KW - Plastid KW - Sesamum indicum KW - ω-3 Fatty acid desaturase SP - 213 EP - 26 JF - Plant cell reports JO - Plant Cell Rep VL - 35 IS - 1 N2 - KEY MESSAGE: Expression of sesame plastidial FAD7 desaturase modified with the endoplasmic reticulum targeting and retention signals, enhances the α-linolenic acid accumulation in seeds of Nicotiana tabacum. In plants, plastidial ω-3 fatty acid desaturase-7 (FAD7) catalyzes the formation of C16 and C18 trienoic fatty acids using organellar glycerolipids and participate in the membrane lipid formation. The plastidial ω-3 desaturases (FAD7) share high sequence homology with the microsomal ω-3 desaturases (FAD3) at the amino acid level except the N-terminal organelle transit peptide. In the present study, the predicted N-terminal plastidial signal peptide of fad7 gene was replaced by the endoplasmic reticulum signal peptide and an endoplasmic reticulum retention signal was placed at the C-terminal. The expression of the modified sesame ω-3 desaturase increases the α-linolenic acid content in the range of 4.78-6.77 % in the seeds of transgenic tobacco plants with concomitant decrease in linoleic acid content. The results suggested the potential of the engineered plastidial ω-3 desaturase from sesame to influence the profile of α-linolenic acid in tobacco plant by shifting the carbon flux from linoleic acid, and thus it can be used in suitable genetic engineering strategy to increase the α-linolenic acid content in sesame and other vegetable oils. SN - 1432-203X UR - https://www.unboundmedicine.com/medline/citation/26521211/Enhancement_of_α_linolenic_acid_content_in_transgenic_tobacco_seeds_by_targeting_a_plastidial_ω_3_fatty_acid_desaturase__fad7__gene_of_Sesamum_indicum_to_ER_ DB - PRIME DP - Unbound Medicine ER -

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Enhancement of α-linolenic acid content in transgenic tobacco seeds by targeting a plastidial ω-3 fatty acid desaturase (fad7) gene of Sesamum indicum to ER.Plant Cell Rep. 2016 Jan; 35(1):213-26.PC