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[Effects of electroacupuncture on the expression of related protein mRNA in the striatum in rats with Parkinson's disease].
Zhongguo Zhen Jiu. 2015 Jul; 35(7):697-701.ZZ

Abstract

OBJECTIVE

To explore action mechanism of electroacupuncture (EA) on treatment of Parkinson's disease (PD).

METHODS

A total of 40 healthy male SD rats were randomly divided into a normal group, a sham operation group, a model group and an EA group, 10 rats in each group. PD rat model was duplicated by micro injection of 6-hydroxyl dopamine into right striatum of rats, and the rats in the sham operation group were treated with micro injection of 0. 9% NaCl. Rats in the normal group, model group and the sham operation group received no treatment; rats in the EA group were treated by EA at "Fengfu" (GV 16) and "Taichong" (LR 3) with continuous wave, 2 Hz in frequency, 1 mA in intensity for 30 min. The treatment was given once a day for total 2 weeks. Behavioral test was used to evaluate rotational behavior changes of PD rats. RT-PCR method was applied to detect the expression of GFAP (glial fiber acidic protein) mRNA and Cx43 (connexin 43) mRNA in the striatum.

RESULTS

The difference of rotational behavior was not significant before and after treatment in the model group (P>0. 05), while that in the EA group was significant (P<0. 01). The expression of GFAP mRNA and Cx43 mRNA in the model group was significantly higher than that in the normal group and sham operation group (all P<0. 01); after EA treatment, the expression of GFAP mRNA and Cx43 mRNA in the EA group was lower significantly than that in the model group (both P<0. 01).

CONCLUSION

The action mechanism of EA for prevention and treatment of Parkinson' s disease may be associated with inhibiting the activation of astrocytes.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26521587

Citation

Wang, Shuju, et al. "[Effects of Electroacupuncture On the Expression of Related Protein mRNA in the Striatum in Rats With Parkinson's Disease]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 35, no. 7, 2015, pp. 697-701.
Wang S, Ma B, Ma J, et al. [Effects of electroacupuncture on the expression of related protein mRNA in the striatum in rats with Parkinson's disease]. Zhongguo Zhen Jiu. 2015;35(7):697-701.
Wang, S., Ma, B., Ma, J., Liu, F., Wang, Y., & Sun, G. (2015). [Effects of electroacupuncture on the expression of related protein mRNA in the striatum in rats with Parkinson's disease]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 35(7), 697-701.
Wang S, et al. [Effects of Electroacupuncture On the Expression of Related Protein mRNA in the Striatum in Rats With Parkinson's Disease]. Zhongguo Zhen Jiu. 2015;35(7):697-701. PubMed PMID: 26521587.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of electroacupuncture on the expression of related protein mRNA in the striatum in rats with Parkinson's disease]. AU - Wang,Shuju, AU - Ma,Biao, AU - Ma,Jun, AU - Liu,Fang, AU - Wang,Yanchun, AU - Sun,Guojie, PY - 2015/11/3/entrez PY - 2015/11/3/pubmed PY - 2015/11/13/medline SP - 697 EP - 701 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 35 IS - 7 N2 - OBJECTIVE: To explore action mechanism of electroacupuncture (EA) on treatment of Parkinson's disease (PD). METHODS: A total of 40 healthy male SD rats were randomly divided into a normal group, a sham operation group, a model group and an EA group, 10 rats in each group. PD rat model was duplicated by micro injection of 6-hydroxyl dopamine into right striatum of rats, and the rats in the sham operation group were treated with micro injection of 0. 9% NaCl. Rats in the normal group, model group and the sham operation group received no treatment; rats in the EA group were treated by EA at "Fengfu" (GV 16) and "Taichong" (LR 3) with continuous wave, 2 Hz in frequency, 1 mA in intensity for 30 min. The treatment was given once a day for total 2 weeks. Behavioral test was used to evaluate rotational behavior changes of PD rats. RT-PCR method was applied to detect the expression of GFAP (glial fiber acidic protein) mRNA and Cx43 (connexin 43) mRNA in the striatum. RESULTS: The difference of rotational behavior was not significant before and after treatment in the model group (P>0. 05), while that in the EA group was significant (P<0. 01). The expression of GFAP mRNA and Cx43 mRNA in the model group was significantly higher than that in the normal group and sham operation group (all P<0. 01); after EA treatment, the expression of GFAP mRNA and Cx43 mRNA in the EA group was lower significantly than that in the model group (both P<0. 01). CONCLUSION: The action mechanism of EA for prevention and treatment of Parkinson' s disease may be associated with inhibiting the activation of astrocytes. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/26521587/[Effects_of_electroacupuncture_on_the_expression_of_related_protein_mRNA_in_the_striatum_in_rats_with_Parkinson's_disease]_ L2 - https://medlineplus.gov/parkinsonsdisease.html DB - PRIME DP - Unbound Medicine ER -