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H2S, a novel gasotransmitter, involves in gastric accommodation.
Sci Rep. 2015 Nov 04; 5:16086.SR

Abstract

H2S is produced mainly by two enzymes:cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE), using L-cysteine (L-Cys) as the substrate. In this study, we investigated the role of H2S in gastric accommodation using CBS(+/-) mice, immunohistochemistry, immunoblot, methylene blue assay, intragastric pressure (IGP) recording and electrical field stimulation (EFS). Mouse gastric fundus expressed H2S-generating enzymes (CBS and CSE) and generated detectable amounts of H2S. The H2S donor, NaHS or L-Cys, caused a relaxation in either gastric fundus or body. The gastric compliance was significantly increased in the presence of L-Cys (1 mM). On the contrary, AOAA, an inhibitor for CBS, largely inhibited gastric compliance. Consistently, CBS(+/-) mice shows a lower gastric compliance. However, PAG, a CSE inhibitor, had no effect on gastric compliances. L-Cys enhances the non-adrenergic, non-cholinergic (NANC) relaxation of fundus strips, but AOAA reduces the magnitude of relaxations to EFS. Notably, the expression level of CBS but not CSE protein was elevated after feeding. Consistently, the production of H2S was also increased after feeding in mice gastric fundus. In addition, AOAA largely reduced food intake and body weight in mice. Furthermore, a metabolic aberration of H2S was found in patients with functional dyspepsia (FD). In conclusion, endogenous H2S, a novel gasotransmitter, involves in gastric accommodation.

Authors+Show Affiliations

Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.Department of gastroenterology, Second Hospital, Shandong University, Jinan, People's Republic of China.Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.Department of gastroenterology, Second Hospital, Shandong University, Jinan, People's Republic of China.Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26531221

Citation

Xiao, Ailin, et al. "H2S, a Novel Gasotransmitter, Involves in Gastric Accommodation." Scientific Reports, vol. 5, 2015, p. 16086.
Xiao A, Wang H, Lu X, et al. H2S, a novel gasotransmitter, involves in gastric accommodation. Sci Rep. 2015;5:16086.
Xiao, A., Wang, H., Lu, X., Zhu, J., Huang, D., Xu, T., Guo, J., Liu, C., & Li, J. (2015). H2S, a novel gasotransmitter, involves in gastric accommodation. Scientific Reports, 5, 16086. https://doi.org/10.1038/srep16086
Xiao A, et al. H2S, a Novel Gasotransmitter, Involves in Gastric Accommodation. Sci Rep. 2015 Nov 4;5:16086. PubMed PMID: 26531221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - H2S, a novel gasotransmitter, involves in gastric accommodation. AU - Xiao,Ailin, AU - Wang,Hongjuan, AU - Lu,Xin, AU - Zhu,Jianchun, AU - Huang,Di, AU - Xu,Tonghui, AU - Guo,Jianqiang, AU - Liu,Chuanyong, AU - Li,Jingxin, Y1 - 2015/11/04/ PY - 2015/07/06/received PY - 2015/10/07/accepted PY - 2015/11/5/entrez PY - 2015/11/5/pubmed PY - 2016/9/30/medline SP - 16086 EP - 16086 JF - Scientific reports JO - Sci Rep VL - 5 N2 - H2S is produced mainly by two enzymes:cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE), using L-cysteine (L-Cys) as the substrate. In this study, we investigated the role of H2S in gastric accommodation using CBS(+/-) mice, immunohistochemistry, immunoblot, methylene blue assay, intragastric pressure (IGP) recording and electrical field stimulation (EFS). Mouse gastric fundus expressed H2S-generating enzymes (CBS and CSE) and generated detectable amounts of H2S. The H2S donor, NaHS or L-Cys, caused a relaxation in either gastric fundus or body. The gastric compliance was significantly increased in the presence of L-Cys (1 mM). On the contrary, AOAA, an inhibitor for CBS, largely inhibited gastric compliance. Consistently, CBS(+/-) mice shows a lower gastric compliance. However, PAG, a CSE inhibitor, had no effect on gastric compliances. L-Cys enhances the non-adrenergic, non-cholinergic (NANC) relaxation of fundus strips, but AOAA reduces the magnitude of relaxations to EFS. Notably, the expression level of CBS but not CSE protein was elevated after feeding. Consistently, the production of H2S was also increased after feeding in mice gastric fundus. In addition, AOAA largely reduced food intake and body weight in mice. Furthermore, a metabolic aberration of H2S was found in patients with functional dyspepsia (FD). In conclusion, endogenous H2S, a novel gasotransmitter, involves in gastric accommodation. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/26531221/H2S_a_novel_gasotransmitter_involves_in_gastric_accommodation_ L2 - https://doi.org/10.1038/srep16086 DB - PRIME DP - Unbound Medicine ER -