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[Development of a Novel Method for Quantifying Quassin and Neoquassin in Jamaica Quassia Extracts Using the Molar Absorption Coefficient Ratio].
Shokuhin Eiseigaku Zasshi. 2015; 56(5):185-93.SE

Abstract

A novel HPLC-based method employing molar absorption coefficient ratios to 4-hydroxybenzoic acid (4HBA) was developed for the determination of quassin and neoquassin in Jamaica quassia extract, which is used as a food additive in Japan. Based on comparisons of quantitative NMR (qNMR) spectra and HPLC chromatograms of an artificial mixture of quassin, neoquassin, and 4HBA, the molar absorption coefficient ratios of quassin and neoquassin to 4HBA were determined as 0.84 and 0.85, respectively. Quassin and neoquassin were quantified in food additives by qNMR and HPLC based on molar absorption coefficient ratios using 1,4-bis(trimethylsilyl)benzene-d4 and 4HBA as internal standards, respectively. The differences in quantitation values between qNMR and HPLC analyses were below 1.2%. Our proposed novel HPLC-based quantitation method employing the molar absorption coefficient ratios is a reliable tool for determining levels of quassin and neoquassin in food additives and processed foods.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Nishizaki Y
National Institute of Health Science.
Tada A
No affiliation info available
Ishizuki K
No affiliation info available
Ito Y
No affiliation info available
Onoda A
No affiliation info available
Sugimoto N
No affiliation info available
Akiyama H
No affiliation info available

MeSH

Chromatography, High Pressure LiquidFood AdditivesFood AnalysisHydroxybenzoatesMagnetic Resonance SpectroscopyPlant ExtractsQuassiaQuassins

Pub Type(s)

Journal Article

Language

jpn

PubMed ID

26537647

Citation

Nishizaki, Yuzo, et al. "[Development of a Novel Method for Quantifying Quassin and Neoquassin in Jamaica Quassia Extracts Using the Molar Absorption Coefficient Ratio]." Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan, vol. 56, no. 5, 2015, pp. 185-93.
Nishizaki Y, Tada A, Ishizuki K, et al. [Development of a Novel Method for Quantifying Quassin and Neoquassin in Jamaica Quassia Extracts Using the Molar Absorption Coefficient Ratio]. Shokuhin Eiseigaku Zasshi. 2015;56(5):185-93.
Nishizaki, Y., Tada, A., Ishizuki, K., Ito, Y., Onoda, A., Sugimoto, N., & Akiyama, H. (2015). [Development of a Novel Method for Quantifying Quassin and Neoquassin in Jamaica Quassia Extracts Using the Molar Absorption Coefficient Ratio]. Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan, 56(5), 185-93. https://doi.org/10.3358/shokueishi.56.185
Nishizaki Y, et al. [Development of a Novel Method for Quantifying Quassin and Neoquassin in Jamaica Quassia Extracts Using the Molar Absorption Coefficient Ratio]. Shokuhin Eiseigaku Zasshi. 2015;56(5):185-93. PubMed PMID: 26537647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Development of a Novel Method for Quantifying Quassin and Neoquassin in Jamaica Quassia Extracts Using the Molar Absorption Coefficient Ratio]. AU - Nishizaki,Yuzo, AU - Tada,Atsuko, AU - Ishizuki,Kyoko, AU - Ito,Yusai, AU - Onoda,Aya, AU - Sugimoto,Naoki, AU - Akiyama,Hiroshi, PY - 2015/11/6/entrez PY - 2015/11/6/pubmed PY - 2016/6/15/medline SP - 185 EP - 93 JF - Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan JO - Shokuhin Eiseigaku Zasshi VL - 56 IS - 5 N2 - A novel HPLC-based method employing molar absorption coefficient ratios to 4-hydroxybenzoic acid (4HBA) was developed for the determination of quassin and neoquassin in Jamaica quassia extract, which is used as a food additive in Japan. Based on comparisons of quantitative NMR (qNMR) spectra and HPLC chromatograms of an artificial mixture of quassin, neoquassin, and 4HBA, the molar absorption coefficient ratios of quassin and neoquassin to 4HBA were determined as 0.84 and 0.85, respectively. Quassin and neoquassin were quantified in food additives by qNMR and HPLC based on molar absorption coefficient ratios using 1,4-bis(trimethylsilyl)benzene-d4 and 4HBA as internal standards, respectively. The differences in quantitation values between qNMR and HPLC analyses were below 1.2%. Our proposed novel HPLC-based quantitation method employing the molar absorption coefficient ratios is a reliable tool for determining levels of quassin and neoquassin in food additives and processed foods. SN - 1882-1006 UR - https://www.unboundmedicine.com/medline/citation/26537647/[Development_of_a_Novel_Method_for_Quantifying_Quassin_and_Neoquassin_in_Jamaica_Quassia_Extracts_Using_the_Molar_Absorption_Coefficient_Ratio]_ DB - PRIME DP - Unbound Medicine ER -