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Multiplex PCR testing for nine different sexually transmitted infections.
Int J STD AIDS. 2016 12; 27(14):1275-1282.IJ

Abstract

Current sexually transmitted infection (STI) testing is not optimal due to delays in reporting or missed diagnoses due to a lack of comprehensive testing. The FilmArray® (BioFire Diagnostics, LLC, Salt Lake City, Utah) is a user-friendly, fully automated, multiplex PCR system that is being developed for rapid point-of-care use. A research-use-only STI panel including multiple PCR primer sets for each organism was designed to detect Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum, Trichomonas vaginalis, Mycoplasma genitalium, Ureaplasma urealyticum, Haemophilus ducreyi, and herpes simplex virus (HSV) types 1 and 2. Standard clinical testing included Gram stain, nucleic acid amplification, wet mount examination, herpes simplex virus culture, and syphilis IgG. Standard clinical tests were not available for all the organisms tested by the FilmArray STI panel. Two hundred and ninety-five clinical specimens from 190 subjects were directly compared to standard testing. Urine (n = 146), urethral/cervical swabs (31), oral swabs (60), rectal swabs (43), and ulcer swabs (15) were tested. Among the tested samples, FilmArray detected C. trachomatis in 39 (13%), N. gonorrhoeae in 20 (7%), T. vaginalis in nine (3%), HSV 1 in five (2%), HSV 2 in five (2%), U. urealyticum in 36 (12%), M. genitalium in eight (3%), and T. pallidum in 11 (4%). Concordance between the FilmArray STI panel and standard nucleic acid amplification testing for C. trachomatis was 98% and for N. gonorrhoeae was 97%. Multiplex PCR STI testing has the potential to improve public health by providing rapid, sensitive, and reliable results within the clinic or nearby laboratory.

Authors+Show Affiliations

Division of Infectious Diseases, Department of Internal Medicine, University of Utah, USA John.Kriesel@hsc.utah.edu.Division of Infectious Diseases, Department of Internal Medicine, University of Utah, USA.Salt Lake Valley Health Department, USA.BioFire Diagnostics, LLC, USA.BioFire Defense, LLC, USA.BioFire Diagnostics, LLC, USA.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

26538551

Citation

Kriesel, John D., et al. "Multiplex PCR Testing for Nine Different Sexually Transmitted Infections." International Journal of STD & AIDS, vol. 27, no. 14, 2016, pp. 1275-1282.
Kriesel JD, Bhatia AS, Barrus C, et al. Multiplex PCR testing for nine different sexually transmitted infections. Int J STD AIDS. 2016;27(14):1275-1282.
Kriesel, J. D., Bhatia, A. S., Barrus, C., Vaughn, M., Gardner, J., & Crisp, R. J. (2016). Multiplex PCR testing for nine different sexually transmitted infections. International Journal of STD & AIDS, 27(14), 1275-1282.
Kriesel JD, et al. Multiplex PCR Testing for Nine Different Sexually Transmitted Infections. Int J STD AIDS. 2016;27(14):1275-1282. PubMed PMID: 26538551.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiplex PCR testing for nine different sexually transmitted infections. AU - Kriesel,John D, AU - Bhatia,Amiteshwar S, AU - Barrus,Cammie, AU - Vaughn,Mike, AU - Gardner,Jordan, AU - Crisp,Robert J, Y1 - 2015/11/03/ PY - 2015/06/24/received PY - 2015/10/14/accepted PY - 2015/11/6/pubmed PY - 2017/7/1/medline PY - 2015/11/6/entrez KW - FilmArray KW - STI KW - diagnostic test performance KW - multiplex PCR KW - sexually transmitted diseases KW - sexually transmitted infections SP - 1275 EP - 1282 JF - International journal of STD & AIDS JO - Int J STD AIDS VL - 27 IS - 14 N2 - Current sexually transmitted infection (STI) testing is not optimal due to delays in reporting or missed diagnoses due to a lack of comprehensive testing. The FilmArray® (BioFire Diagnostics, LLC, Salt Lake City, Utah) is a user-friendly, fully automated, multiplex PCR system that is being developed for rapid point-of-care use. A research-use-only STI panel including multiple PCR primer sets for each organism was designed to detect Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum, Trichomonas vaginalis, Mycoplasma genitalium, Ureaplasma urealyticum, Haemophilus ducreyi, and herpes simplex virus (HSV) types 1 and 2. Standard clinical testing included Gram stain, nucleic acid amplification, wet mount examination, herpes simplex virus culture, and syphilis IgG. Standard clinical tests were not available for all the organisms tested by the FilmArray STI panel. Two hundred and ninety-five clinical specimens from 190 subjects were directly compared to standard testing. Urine (n = 146), urethral/cervical swabs (31), oral swabs (60), rectal swabs (43), and ulcer swabs (15) were tested. Among the tested samples, FilmArray detected C. trachomatis in 39 (13%), N. gonorrhoeae in 20 (7%), T. vaginalis in nine (3%), HSV 1 in five (2%), HSV 2 in five (2%), U. urealyticum in 36 (12%), M. genitalium in eight (3%), and T. pallidum in 11 (4%). Concordance between the FilmArray STI panel and standard nucleic acid amplification testing for C. trachomatis was 98% and for N. gonorrhoeae was 97%. Multiplex PCR STI testing has the potential to improve public health by providing rapid, sensitive, and reliable results within the clinic or nearby laboratory. SN - 1758-1052 UR - https://www.unboundmedicine.com/medline/citation/26538551/Multiplex_PCR_testing_for_nine_different_sexually_transmitted_infections_ L2 - https://journals.sagepub.com/doi/10.1177/0956462415615775?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -