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[Role of transient receptor potential canonical 1 in airway remodeling and effect of budesonide on its pulmonary expression in asthmatic guinea pigs].
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Oct; 35(10):1374-9.NF

Abstract

OBJECTIVE

To explore the role of transient receptor potential canonical 1 (TRPC1) in airway remodeling and the effect of budesonide intervention on its expression in the lungs of guinea pigs with ovalbumin-induced asthma.

METHODS

Fifty male guinea pigs were randomized into 5 equal groups, including a blank control group, ovalbumin group, ovalbumin+TRPC1 siRNA group, ovalbumin+luciferase siRNA group, and ovalbumin+budesonide group. After corresponding treatments, bronchoalveolar lavage was collected from the guinea pigs for eosinophils analysis and detection of IL-5 and IL-13 levels using ELISA. The lung tissues were stained with HE and Masson's trichrome to observe the bronchial wall thickness, smooth muscle hypertrophy, subepithelial collagen deposition, and lung inflammations. Immunohistochemistry and real-time quantitative PCR were performed to detect TRPC1 protein and mRNA expressions in the lungs, respectively.

RESULTS

The guinea pig models of ovalbumin-induced asthma showed significantly increased thickness of the bronchial wall, smooth muscle hypertrophy, collagen deposition and inflammatory cell infiltration, but these pathologies were obviously alleviated by treatment with TRPC1 siRNA or budesonide (P/0.05). Immunohistochemstry showed that TRPC1 protein was distributed mainly on the cell membrane and in the nuclei of the basal cells or columnar epithelial cells.

CONCLUSION

The up-regulated expression of TRPC1 ion channel is closely associated with the occurrence and progression of airway remodeling and chronic airway inflammation in asthma. Budesonide can partially suppress airway remodeling and inflammation by regulating the expression of TRPC1.

Authors+Show Affiliations

Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China. E-mail: linamu90@163.com.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26547326

Citation

Li, Na, et al. "[Role of Transient Receptor Potential Canonical 1 in Airway Remodeling and Effect of Budesonide On Its Pulmonary Expression in Asthmatic Guinea Pigs]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 35, no. 10, 2015, pp. 1374-9.
Li N, He Y, Li MC. [Role of transient receptor potential canonical 1 in airway remodeling and effect of budesonide on its pulmonary expression in asthmatic guinea pigs]. Nan Fang Yi Ke Da Xue Xue Bao. 2015;35(10):1374-9.
Li, N., He, Y., & Li, M. C. (2015). [Role of transient receptor potential canonical 1 in airway remodeling and effect of budesonide on its pulmonary expression in asthmatic guinea pigs]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 35(10), 1374-9.
Li N, He Y, Li MC. [Role of Transient Receptor Potential Canonical 1 in Airway Remodeling and Effect of Budesonide On Its Pulmonary Expression in Asthmatic Guinea Pigs]. Nan Fang Yi Ke Da Xue Xue Bao. 2015;35(10):1374-9. PubMed PMID: 26547326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Role of transient receptor potential canonical 1 in airway remodeling and effect of budesonide on its pulmonary expression in asthmatic guinea pigs]. AU - Li,Na, AU - He,Ye, AU - Li,Min-Chao, PY - 2015/11/9/entrez PY - 2015/11/10/pubmed PY - 2016/1/26/medline SP - 1374 EP - 9 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 35 IS - 10 N2 - OBJECTIVE: To explore the role of transient receptor potential canonical 1 (TRPC1) in airway remodeling and the effect of budesonide intervention on its expression in the lungs of guinea pigs with ovalbumin-induced asthma. METHODS: Fifty male guinea pigs were randomized into 5 equal groups, including a blank control group, ovalbumin group, ovalbumin+TRPC1 siRNA group, ovalbumin+luciferase siRNA group, and ovalbumin+budesonide group. After corresponding treatments, bronchoalveolar lavage was collected from the guinea pigs for eosinophils analysis and detection of IL-5 and IL-13 levels using ELISA. The lung tissues were stained with HE and Masson's trichrome to observe the bronchial wall thickness, smooth muscle hypertrophy, subepithelial collagen deposition, and lung inflammations. Immunohistochemistry and real-time quantitative PCR were performed to detect TRPC1 protein and mRNA expressions in the lungs, respectively. RESULTS: The guinea pig models of ovalbumin-induced asthma showed significantly increased thickness of the bronchial wall, smooth muscle hypertrophy, collagen deposition and inflammatory cell infiltration, but these pathologies were obviously alleviated by treatment with TRPC1 siRNA or budesonide (P/0.05). Immunohistochemstry showed that TRPC1 protein was distributed mainly on the cell membrane and in the nuclei of the basal cells or columnar epithelial cells. CONCLUSION: The up-regulated expression of TRPC1 ion channel is closely associated with the occurrence and progression of airway remodeling and chronic airway inflammation in asthma. Budesonide can partially suppress airway remodeling and inflammation by regulating the expression of TRPC1. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/26547326/[Role_of_transient_receptor_potential_canonical_1_in_airway_remodeling_and_effect_of_budesonide_on_its_pulmonary_expression_in_asthmatic_guinea_pigs]_ L2 - https://medlineplus.gov/asthma.html DB - PRIME DP - Unbound Medicine ER -