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Identification of specific antinuclear antibodies in dogs using a line immunoassay and enzyme-linked immunosorbent assay.
Vet Immunol Immunopathol. 2015 Dec 15; 168(3-4):233-41.VI

Abstract

Circulating antinuclear antibodies (ANA) are commonly present in the systemic autoimmune disease Systemic Lupus Erythematosus (SLE) and in other systemic rheumatic diseases, in humans as well as in dogs. The indirect immunofluorescence (IIF)-ANA test is the standard method for detecting ANA. Further testing for specific ANA with immunoblot techniques or ELISAs is routinely performed in humans to aid in the diagnosis and monitoring of disease. Several specific ANA identified in humans have been identified also in suspected canine SLE but, in contrast to humans, investigation of autoantibodies in canine SLE is mainly restricted to the IIF-ANA test. Our aim was to identify both known and novel specific ANA in dogs and to investigate if different IIF-ANA patterns are associated with different specific ANA in dogs. Sera from 240 dogs with suspicion of autoimmune disease (210 IIF-ANA positive (ANA(pos)) and 30 IIF-ANA negative (ANA(neg))) as well as sera from 27 healthy controls were included. The samples were analysed with a line immunoassay, LIA (Euroline ANA Profile 5, Euroimmun, Lübeck, Germany) and four different ELISAs (Euroimmun). The ANA(pos) dogs were divided in two groups depending on the type of IIF-ANA pattern. Of the 210 ANA(pos) samples 68 were classified as ANA homogenous (ANA(H)) and 141 as ANA speckled (ANA(S)), one sample was not possible to classify. Dogs in the ANA(H) group had, compared to the other groups, most frequently high levels of anti-double stranded deoxyribonucleic acid (dsDNA) and anti-nucleosome ANA. Anti-dsDNA antibodies were confirmed in some dogs with the Crithidia luciliae indirect immunofluorescence test (CLIFT). The frequency of ANA(H) dogs with values above those observed in the healthy group was significantly higher compared to ANA(S) dogs for anti-dsDNA, anti-nucleosome, and anti-histone reactivity. Dogs in the ANA(S) group had, compared to the other groups, most frequently high levels of anti-ribonucleoproteins (RNP) and/or anti-Smith (Sm) antibodies. Reactivity against Sjögren's syndrome related antigens (SS)-A (including the Ro-60 and Ro-52 subcomponents), SS-B, histidyl tRNA synthetase (Jo-1), topoisomerase I antigen (Scl-70), polymyositis-scleroderma antigen (PM-Scl) and proliferating cell nuclear antigen (PCNA) was also noted in individual dogs. In conclusion, by using a commercial LIA and different ELISAs originally developed for detection of human ANA, we identified several specific ANA in serum samples from dogs sampled for IIF-ANA testing. Further, we found that the types of IIF-ANA pattern were associated with reactivity against some particular nuclear antigens.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Bremer HD
Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Box 7054, SE-750 07 Uppsala, Sweden. Electronic address: hanna.bremer@slu.se.
Lattwein E
Euroimmun AG, Lübeck, Germany.
Renneker S
Euroimmun AG, Lübeck, Germany.
Lilliehöök I
Clinical Pathology Laboratory, University Animal Hospital, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Rönnelid J
Department of Immunology, Genetics and Pathology, Uppsala University, Sweden.
Hansson-Hamlin H
Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Box 7054, SE-750 07 Uppsala, Sweden.

MeSH

AnimalsAntibodies, AntinuclearAntibody SpecificityAutoimmune DiseasesDNADog DiseasesDogsEnzyme-Linked Immunosorbent AssayHistonesImmunoassayNucleosomes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26547884

Citation

Bremer, Hanna D., et al. "Identification of Specific Antinuclear Antibodies in Dogs Using a Line Immunoassay and Enzyme-linked Immunosorbent Assay." Veterinary Immunology and Immunopathology, vol. 168, no. 3-4, 2015, pp. 233-41.
Bremer HD, Lattwein E, Renneker S, et al. Identification of specific antinuclear antibodies in dogs using a line immunoassay and enzyme-linked immunosorbent assay. Vet Immunol Immunopathol. 2015;168(3-4):233-41.
Bremer, H. D., Lattwein, E., Renneker, S., Lilliehöök, I., Rönnelid, J., & Hansson-Hamlin, H. (2015). Identification of specific antinuclear antibodies in dogs using a line immunoassay and enzyme-linked immunosorbent assay. Veterinary Immunology and Immunopathology, 168(3-4), 233-41. https://doi.org/10.1016/j.vetimm.2015.10.002
Bremer HD, et al. Identification of Specific Antinuclear Antibodies in Dogs Using a Line Immunoassay and Enzyme-linked Immunosorbent Assay. Vet Immunol Immunopathol. 2015 Dec 15;168(3-4):233-41. PubMed PMID: 26547884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of specific antinuclear antibodies in dogs using a line immunoassay and enzyme-linked immunosorbent assay. AU - Bremer,Hanna D, AU - Lattwein,Erik, AU - Renneker,Stefanie, AU - Lilliehöök,Inger, AU - Rönnelid,Johan, AU - Hansson-Hamlin,Helene, Y1 - 2015/10/09/ PY - 2015/5/6/received PY - 2015/10/1/revised PY - 2015/10/4/accepted PY - 2015/11/9/entrez PY - 2015/11/9/pubmed PY - 2016/10/12/medline KW - Antinuclear antibodies KW - Diagnostics KW - Dog KW - Systemic Lupus Erythematosus SP - 233 EP - 41 JF - Veterinary immunology and immunopathology JO - Vet Immunol Immunopathol VL - 168 IS - 3-4 N2 - Circulating antinuclear antibodies (ANA) are commonly present in the systemic autoimmune disease Systemic Lupus Erythematosus (SLE) and in other systemic rheumatic diseases, in humans as well as in dogs. The indirect immunofluorescence (IIF)-ANA test is the standard method for detecting ANA. Further testing for specific ANA with immunoblot techniques or ELISAs is routinely performed in humans to aid in the diagnosis and monitoring of disease. Several specific ANA identified in humans have been identified also in suspected canine SLE but, in contrast to humans, investigation of autoantibodies in canine SLE is mainly restricted to the IIF-ANA test. Our aim was to identify both known and novel specific ANA in dogs and to investigate if different IIF-ANA patterns are associated with different specific ANA in dogs. Sera from 240 dogs with suspicion of autoimmune disease (210 IIF-ANA positive (ANA(pos)) and 30 IIF-ANA negative (ANA(neg))) as well as sera from 27 healthy controls were included. The samples were analysed with a line immunoassay, LIA (Euroline ANA Profile 5, Euroimmun, Lübeck, Germany) and four different ELISAs (Euroimmun). The ANA(pos) dogs were divided in two groups depending on the type of IIF-ANA pattern. Of the 210 ANA(pos) samples 68 were classified as ANA homogenous (ANA(H)) and 141 as ANA speckled (ANA(S)), one sample was not possible to classify. Dogs in the ANA(H) group had, compared to the other groups, most frequently high levels of anti-double stranded deoxyribonucleic acid (dsDNA) and anti-nucleosome ANA. Anti-dsDNA antibodies were confirmed in some dogs with the Crithidia luciliae indirect immunofluorescence test (CLIFT). The frequency of ANA(H) dogs with values above those observed in the healthy group was significantly higher compared to ANA(S) dogs for anti-dsDNA, anti-nucleosome, and anti-histone reactivity. Dogs in the ANA(S) group had, compared to the other groups, most frequently high levels of anti-ribonucleoproteins (RNP) and/or anti-Smith (Sm) antibodies. Reactivity against Sjögren's syndrome related antigens (SS)-A (including the Ro-60 and Ro-52 subcomponents), SS-B, histidyl tRNA synthetase (Jo-1), topoisomerase I antigen (Scl-70), polymyositis-scleroderma antigen (PM-Scl) and proliferating cell nuclear antigen (PCNA) was also noted in individual dogs. In conclusion, by using a commercial LIA and different ELISAs originally developed for detection of human ANA, we identified several specific ANA in serum samples from dogs sampled for IIF-ANA testing. Further, we found that the types of IIF-ANA pattern were associated with reactivity against some particular nuclear antigens. SN - 1873-2534 UR - https://www.unboundmedicine.com/medline/citation/26547884/Identification_of_specific_antinuclear_antibodies_in_dogs_using_a_line_immunoassay_and_enzyme_linked_immunosorbent_assay_ DB - PRIME DP - Unbound Medicine ER -