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Neuroprotection and immunomodulation by xenografted human mesenchymal stem cells following spinal cord ventral root avulsion.
Sci Rep. 2015 Nov 09; 5:16167.SR

Abstract

The present study investigates the effects of xenotransplantation of Adipose Tissue Mesenchymal Stem Cells (AT-MSCs) in animals after ventral root avulsion. AT-MSC has similar characteristics to bone marrow mesenchymal stem cells (BM-MSCs), such as immunomodulatory properties and expression of neurotrophic factors. In this study, Lewis rats were submitted to surgery for unilateral avulsion of the lumbar ventral roots and received 5 × 10(5) AT-MSCs via the lateral funiculus. Two weeks after cell administration, the animals were sacrificed and the moto neurons, T lymphocytes and cell defense nervous system were analyzed. An increased neuronal survival and partial preservation of synaptophysin-positive nerve terminals, related to GDNF and BDNF expression of AT-MSCs, and reduction of pro-inflammatory reaction were observed. In conclusion, AT-MSCs prevent second phase neuronal injury, since they suppressed lymphocyte, astroglia and microglia effects, which finally contributed to rat motor-neuron survival and synaptic stability of the lesioned motor-neuron. Moreover, the survival of the injected AT- MSCs lasted for at least 14 days. These results indicate that neuronal survival after lesion, followed by mesenchymal stem cell (MSC) administration, might occur through cytokine release and immunomodulation, thus suggesting that AT-MSCs are promising cells for the therapy of neuronal lesions.

Authors+Show Affiliations

Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil. Neuroimmunomodulation Group, Dept. Genetics, Evolution and Bioagents, University of Campinas, Campinas, Brazil.Neuroimmunomodulation Group, Dept. Genetics, Evolution and Bioagents, University of Campinas, Campinas, Brazil.Neuroimmunomodulation Group, Dept. Genetics, Evolution and Bioagents, University of Campinas, Campinas, Brazil.Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.Dept. of Structural and Functional Biology, Institute of Biology, University of Campinas, Campinas, Brazil.Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26548646

Citation

Ribeiro, Thiago B., et al. "Neuroprotection and Immunomodulation By Xenografted Human Mesenchymal Stem Cells Following Spinal Cord Ventral Root Avulsion." Scientific Reports, vol. 5, 2015, p. 16167.
Ribeiro TB, Duarte AS, Longhini AL, et al. Neuroprotection and immunomodulation by xenografted human mesenchymal stem cells following spinal cord ventral root avulsion. Sci Rep. 2015;5:16167.
Ribeiro, T. B., Duarte, A. S., Longhini, A. L., Pradella, F., Farias, A. S., Luzo, A. C., Oliveira, A. L., & Olalla Saad, S. T. (2015). Neuroprotection and immunomodulation by xenografted human mesenchymal stem cells following spinal cord ventral root avulsion. Scientific Reports, 5, 16167. https://doi.org/10.1038/srep16167
Ribeiro TB, et al. Neuroprotection and Immunomodulation By Xenografted Human Mesenchymal Stem Cells Following Spinal Cord Ventral Root Avulsion. Sci Rep. 2015 Nov 9;5:16167. PubMed PMID: 26548646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotection and immunomodulation by xenografted human mesenchymal stem cells following spinal cord ventral root avulsion. AU - Ribeiro,Thiago B, AU - Duarte,Adriana S S, AU - Longhini,Ana Leda F, AU - Pradella,Fernando, AU - Farias,Alessandro S, AU - Luzo,Angela C M, AU - Oliveira,Alexandre L R, AU - Olalla Saad,Sara Teresinha, Y1 - 2015/11/09/ PY - 2014/11/07/received PY - 2015/10/07/accepted PY - 2015/11/10/entrez PY - 2015/11/10/pubmed PY - 2016/9/8/medline SP - 16167 EP - 16167 JF - Scientific reports JO - Sci Rep VL - 5 N2 - The present study investigates the effects of xenotransplantation of Adipose Tissue Mesenchymal Stem Cells (AT-MSCs) in animals after ventral root avulsion. AT-MSC has similar characteristics to bone marrow mesenchymal stem cells (BM-MSCs), such as immunomodulatory properties and expression of neurotrophic factors. In this study, Lewis rats were submitted to surgery for unilateral avulsion of the lumbar ventral roots and received 5 × 10(5) AT-MSCs via the lateral funiculus. Two weeks after cell administration, the animals were sacrificed and the moto neurons, T lymphocytes and cell defense nervous system were analyzed. An increased neuronal survival and partial preservation of synaptophysin-positive nerve terminals, related to GDNF and BDNF expression of AT-MSCs, and reduction of pro-inflammatory reaction were observed. In conclusion, AT-MSCs prevent second phase neuronal injury, since they suppressed lymphocyte, astroglia and microglia effects, which finally contributed to rat motor-neuron survival and synaptic stability of the lesioned motor-neuron. Moreover, the survival of the injected AT- MSCs lasted for at least 14 days. These results indicate that neuronal survival after lesion, followed by mesenchymal stem cell (MSC) administration, might occur through cytokine release and immunomodulation, thus suggesting that AT-MSCs are promising cells for the therapy of neuronal lesions. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/26548646/Neuroprotection_and_immunomodulation_by_xenografted_human_mesenchymal_stem_cells_following_spinal_cord_ventral_root_avulsion_ L2 - http://dx.doi.org/10.1038/srep16167 DB - PRIME DP - Unbound Medicine ER -