Tags

Type your tag names separated by a space and hit enter

Antimutagenicity and induction of antioxidant defense by flavonoid rich extract of Myrcia bella Cambess. in normal and tumor gastric cells.
J Ethnopharmacol 2015; 176:345-55JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The Brazilian "Cerrado" is an important source of natural products, such as Myrcia bella Cambess (MB, also known as "mercurinho"). MB leaves are popularly used for the treatment of diabetes and gastrointestinal disorders; however, only its hypoglycemic activity has been experimentally described.

AIM OF THE STUDY

Because MB is used to treat gastrointestinal disorders, the present study characterized biological activities of hydroalcoholic MB extract in human normal and tumor gastric cells.

MATERIALS AND METHODS

Cytotoxic, antiproliferative, genotoxic and protective effects were evaluated, as well as the effects of the MB extract on gene expression.

RESULTS

The MB extract induced cytotoxicity in tumor cells at lower concentrations compared with normal cells as assessed by the MTT assay. Moreover, the MB extract induced necrosis based on acridine orange/ethidium bromide staining. An antiproliferative effect was evidenced through an arrest in the G2/M phase detected by flow cytometry and a decrease in the nuclear division index using the cytokinesis-block micronucleus cytome assay. Cells treated with MB extract combined with doxorubicin (DXR) showed increased NUBDs, which may be related to the gene amplification of CCND1. Antimutagenic effects were also observed and may be associated with the antioxidant activities detected using the CM-H2DCFDA probe.

CONCLUSIONS

Our findings showed the following: (a) high concentrations of MB induced cytotoxicity and cell death by necrosis; (b) its antiproliferative effect was associated with G2/M arrest; and (c) its antioxidant activity could be responsible for the observed antimutagenic effects and for protective effects against gastrointestinal disorders previously described to MB. Although these effects are not specific to normal or tumor cells, they provide a panel of biological activities for further exploration.

Authors+Show Affiliations

Department of Biological Sciences, Faculty of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14801-902, Brazil. Electronic address: julianaserpeloni@yahoo.com.br.Department of General Biology, Center of Biological Sciences, State University of Londrina (UEL), Londrina 86057-970, Brazil.Department of General Biology, Center of Biological Sciences, State University of Londrina (UEL), Londrina 86057-970, Brazil.Department of General Biology, Center of Biological Sciences, State University of Londrina (UEL), Londrina 86057-970, Brazil.Experimental Campus of São Vicente, São Paulo State University (UNESP), São Vicente 11350-000, Brazil.Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, Uruguay.Department of Biological Sciences, Faculty of Sciences, São Paulo State University (UNESP), Bauru 17033-360, Brazil.Department of Biological Sciences, Faculty of Sciences, São Paulo State University (UNESP), Bauru 17033-360, Brazil; Department of Botany, Institute of Bioscience, São Paulo State University (UNESP), Botucatu 18618-970, Brazil.Department of General Biology, Center of Biological Sciences, State University of Londrina (UEL), Londrina 86057-970, Brazil.Department of Biological Sciences, Faculty of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14801-902, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26549270

Citation

Serpeloni, Juliana Mara, et al. "Antimutagenicity and Induction of Antioxidant Defense By Flavonoid Rich Extract of Myrcia Bella Cambess. in Normal and Tumor Gastric Cells." Journal of Ethnopharmacology, vol. 176, 2015, pp. 345-55.
Serpeloni JM, Specian AF, Ribeiro DL, et al. Antimutagenicity and induction of antioxidant defense by flavonoid rich extract of Myrcia bella Cambess. in normal and tumor gastric cells. J Ethnopharmacol. 2015;176:345-55.
Serpeloni, J. M., Specian, A. F., Ribeiro, D. L., Tuttis, K., Vilegas, W., Martínez-López, W., ... Varanda, E. A. (2015). Antimutagenicity and induction of antioxidant defense by flavonoid rich extract of Myrcia bella Cambess. in normal and tumor gastric cells. Journal of Ethnopharmacology, 176, pp. 345-55. doi:10.1016/j.jep.2015.11.003.
Serpeloni JM, et al. Antimutagenicity and Induction of Antioxidant Defense By Flavonoid Rich Extract of Myrcia Bella Cambess. in Normal and Tumor Gastric Cells. J Ethnopharmacol. 2015 Dec 24;176:345-55. PubMed PMID: 26549270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimutagenicity and induction of antioxidant defense by flavonoid rich extract of Myrcia bella Cambess. in normal and tumor gastric cells. AU - Serpeloni,Juliana Mara, AU - Specian,Ana Flávia Leal, AU - Ribeiro,Diego Luis, AU - Tuttis,Katiuska, AU - Vilegas,Wagner, AU - Martínez-López,Wilner, AU - Dokkedal,Anne Lígia, AU - Saldanha,Luiz Leonardo, AU - Cólus,Ilce Mara de Syllos, AU - Varanda,Eliana Aparecida, Y1 - 2015/11/06/ PY - 2015/06/02/received PY - 2015/10/14/revised PY - 2015/11/01/accepted PY - 2015/11/10/entrez PY - 2015/11/10/pubmed PY - 2016/10/7/medline KW - Acridine orange (PubChem CID: 62344) KW - Antioxidant KW - Antiproliferative KW - Azure A (PubChem CID: 13735) KW - Bromophenol blue (PubChem CID: 8272) KW - Cytochalasin B (PubChem CID: 5311281) KW - Dimethyl sulfoxide (PubChem CID: 679) KW - Doxorubicin (PubChem CID: 31703) KW - Ethidium bromide (PubChem CID: 14710) KW - Gastric disorders KW - Hydrogen peroxide (PubChem CID: 784) KW - Mutagenicity KW - Myrcia bella KW - Necrosis KW - Propidium iodide (PubChem CID: 104981) KW - Thiazolyl blue (PubChem CID: 64965) SP - 345 EP - 55 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 176 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The Brazilian "Cerrado" is an important source of natural products, such as Myrcia bella Cambess (MB, also known as "mercurinho"). MB leaves are popularly used for the treatment of diabetes and gastrointestinal disorders; however, only its hypoglycemic activity has been experimentally described. AIM OF THE STUDY: Because MB is used to treat gastrointestinal disorders, the present study characterized biological activities of hydroalcoholic MB extract in human normal and tumor gastric cells. MATERIALS AND METHODS: Cytotoxic, antiproliferative, genotoxic and protective effects were evaluated, as well as the effects of the MB extract on gene expression. RESULTS: The MB extract induced cytotoxicity in tumor cells at lower concentrations compared with normal cells as assessed by the MTT assay. Moreover, the MB extract induced necrosis based on acridine orange/ethidium bromide staining. An antiproliferative effect was evidenced through an arrest in the G2/M phase detected by flow cytometry and a decrease in the nuclear division index using the cytokinesis-block micronucleus cytome assay. Cells treated with MB extract combined with doxorubicin (DXR) showed increased NUBDs, which may be related to the gene amplification of CCND1. Antimutagenic effects were also observed and may be associated with the antioxidant activities detected using the CM-H2DCFDA probe. CONCLUSIONS: Our findings showed the following: (a) high concentrations of MB induced cytotoxicity and cell death by necrosis; (b) its antiproliferative effect was associated with G2/M arrest; and (c) its antioxidant activity could be responsible for the observed antimutagenic effects and for protective effects against gastrointestinal disorders previously described to MB. Although these effects are not specific to normal or tumor cells, they provide a panel of biological activities for further exploration. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/26549270/Antimutagenicity_and_induction_of_antioxidant_defense_by_flavonoid_rich_extract_of_Myrcia_bella_Cambess__in_normal_and_tumor_gastric_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)30203-8 DB - PRIME DP - Unbound Medicine ER -