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Comparison of virulence between Paracoccidioides brasiliensis and Paracoccidioides lutzii using Galleria mellonella as a host model.
Virulence. 2015; 6(8):766-76.V

Abstract

Paracoccidioidomycosis is a systemic mycosis, endemic in Latin America. The etiologic agents of this mycosis are composed of 2 species: Paracoccidioides brasiliensis and P. lutzii. Murine animal models are the gold standard for in vivo studies; however, ethical, economical and logistical considerations limit their use. Galleria mellonella is a suitable model for in vivo studies of fungal infections. In this study, we compared the virulence of P. brasiliensis and P. lutzii in G. mellonella model. The deaths of larvae infected with P. brasiliensis or P. lutzii were similar, and both species were able to reduce the number of hemocytes, which were estimated by microscopy and flow cytometer. Additionally, the phagocytosis percentage was similar for both species, but when we analyze hemocyte-Paracoccidioides spp. interaction using flow cytometer, P. lutzii showed higher interactions with hemocytes. The gene expression of gp43 as well as this protein was higher for P. lutzii, and this expression may contribute to a greater adherence to hemocytes. These results helped us evaluate the behavior of Paracoccidioides spp in G. mellonella, which is a convenient model for investigating the host-Paracoccidioides spp. interaction.

Authors+Show Affiliations

a Faculdade de Ciências Farmacêuticas; UNESP-Univ Estadual Paulista; Campus Araraquara; Departamento de Análises Clínicas e Núcleo de Proteômica ; Laboratório de Micologia Clínica ; Araraquara , São Paulo , Brazil.a Faculdade de Ciências Farmacêuticas; UNESP-Univ Estadual Paulista; Campus Araraquara; Departamento de Análises Clínicas e Núcleo de Proteômica ; Laboratório de Micologia Clínica ; Araraquara , São Paulo , Brazil.a Faculdade de Ciências Farmacêuticas; UNESP-Univ Estadual Paulista; Campus Araraquara; Departamento de Análises Clínicas e Núcleo de Proteômica ; Laboratório de Micologia Clínica ; Araraquara , São Paulo , Brazil.a Faculdade de Ciências Farmacêuticas; UNESP-Univ Estadual Paulista; Campus Araraquara; Departamento de Análises Clínicas e Núcleo de Proteômica ; Laboratório de Micologia Clínica ; Araraquara , São Paulo , Brazil.a Faculdade de Ciências Farmacêuticas; UNESP-Univ Estadual Paulista; Campus Araraquara; Departamento de Análises Clínicas e Núcleo de Proteômica ; Laboratório de Micologia Clínica ; Araraquara , São Paulo , Brazil.a Faculdade de Ciências Farmacêuticas; UNESP-Univ Estadual Paulista; Campus Araraquara; Departamento de Análises Clínicas e Núcleo de Proteômica ; Laboratório de Micologia Clínica ; Araraquara , São Paulo , Brazil.a Faculdade de Ciências Farmacêuticas; UNESP-Univ Estadual Paulista; Campus Araraquara; Departamento de Análises Clínicas e Núcleo de Proteômica ; Laboratório de Micologia Clínica ; Araraquara , São Paulo , Brazil.a Faculdade de Ciências Farmacêuticas; UNESP-Univ Estadual Paulista; Campus Araraquara; Departamento de Análises Clínicas e Núcleo de Proteômica ; Laboratório de Micologia Clínica ; Araraquara , São Paulo , Brazil.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26552324

Citation

Scorzoni, Liliana, et al. "Comparison of Virulence Between Paracoccidioides Brasiliensis and Paracoccidioides Lutzii Using Galleria Mellonella as a Host Model." Virulence, vol. 6, no. 8, 2015, pp. 766-76.
Scorzoni L, de Paula e Silva AC, Singulani Jde L, et al. Comparison of virulence between Paracoccidioides brasiliensis and Paracoccidioides lutzii using Galleria mellonella as a host model. Virulence. 2015;6(8):766-76.
Scorzoni, L., de Paula e Silva, A. C., Singulani, J. d. e. . L., Leite, F. S., de Oliveira, H. C., da Silva, R. A., Fusco-Almeida, A. M., & Mendes-Giannini, M. J. (2015). Comparison of virulence between Paracoccidioides brasiliensis and Paracoccidioides lutzii using Galleria mellonella as a host model. Virulence, 6(8), 766-76. https://doi.org/10.1080/21505594.2015.1085277
Scorzoni L, et al. Comparison of Virulence Between Paracoccidioides Brasiliensis and Paracoccidioides Lutzii Using Galleria Mellonella as a Host Model. Virulence. 2015;6(8):766-76. PubMed PMID: 26552324.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of virulence between Paracoccidioides brasiliensis and Paracoccidioides lutzii using Galleria mellonella as a host model. AU - Scorzoni,Liliana, AU - de Paula e Silva,Ana Carolina Alves, AU - Singulani,Junya de Lacorte, AU - Leite,Fernanda Sangalli, AU - de Oliveira,Haroldo Cesar, AU - da Silva,Rosangela Aparecida Moraes, AU - Fusco-Almeida,Ana Marisa, AU - Mendes-Giannini,Maria José Soares, Y1 - 2015/11/09/ PY - 2015/11/11/entrez PY - 2015/11/11/pubmed PY - 2016/10/8/medline KW - Galleria mellonella KW - Paracoccidioides spp KW - adhesin KW - adhesion KW - gp43 KW - hemocyte KW - virulence SP - 766 EP - 76 JF - Virulence JO - Virulence VL - 6 IS - 8 N2 - Paracoccidioidomycosis is a systemic mycosis, endemic in Latin America. The etiologic agents of this mycosis are composed of 2 species: Paracoccidioides brasiliensis and P. lutzii. Murine animal models are the gold standard for in vivo studies; however, ethical, economical and logistical considerations limit their use. Galleria mellonella is a suitable model for in vivo studies of fungal infections. In this study, we compared the virulence of P. brasiliensis and P. lutzii in G. mellonella model. The deaths of larvae infected with P. brasiliensis or P. lutzii were similar, and both species were able to reduce the number of hemocytes, which were estimated by microscopy and flow cytometer. Additionally, the phagocytosis percentage was similar for both species, but when we analyze hemocyte-Paracoccidioides spp. interaction using flow cytometer, P. lutzii showed higher interactions with hemocytes. The gene expression of gp43 as well as this protein was higher for P. lutzii, and this expression may contribute to a greater adherence to hemocytes. These results helped us evaluate the behavior of Paracoccidioides spp in G. mellonella, which is a convenient model for investigating the host-Paracoccidioides spp. interaction. SN - 2150-5608 UR - https://www.unboundmedicine.com/medline/citation/26552324/Comparison_of_virulence_between_Paracoccidioides_brasiliensis_and_Paracoccidioides_lutzii_using_Galleria_mellonella_as_a_host_model_ L2 - http://www.tandfonline.com/doi/full/10.1080/21505594.2015.1085277 DB - PRIME DP - Unbound Medicine ER -