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Development and Validation of Liquid Chromatographic Method for Estimation of Naringin in Nanoformulation.
J Pharm (Cairo). 2014; 2014:864901.JP

Abstract

A simple, precise, accurate, rapid, and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method with UV detection has been developed and validated for quantification of naringin (NAR) in novel pharmaceutical formulation. NAR is a polyphenolic flavonoid present in most of the citrus plants having variety of pharmacological activities. Method optimization was carried out by considering the various parameters such as effect of pH and column. The analyte was separated by employing a C18 (250.0 × 4.6 mm, 5 μm) column at ambient temperature in isocratic conditions using phosphate buffer pH 3.5: acetonitrile (75 : 25% v/v) as mobile phase pumped at a flow rate of 1.0 mL/min. UV detection was carried out at 282 nm. The developed method was validated according to ICH guidelines Q2(R1). The method was found to be precise and accurate on statistical evaluation with a linearity range of 0.1 to 20.0 μg/mL for NAR. The intra- and interday precision studies showed good reproducibility with coefficients of variation (CV) less than 1.0%. The mean recovery of NAR was found to be 99.33 ± 0.16%. The proposed method was found to be highly accurate, sensitive, and robust. The proposed liquid chromatographic method was successfully employed for the routine analysis of said compound in developed novel nanopharmaceuticals. The presence of excipients did not show any interference on the determination of NAR, indicating method specificity.

Authors+Show Affiliations

Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India.Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India.Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India.Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India.Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India.Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India.Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26556205

Citation

Musmade, Kranti P., et al. "Development and Validation of Liquid Chromatographic Method for Estimation of Naringin in Nanoformulation." Journal of Pharmaceutics, vol. 2014, 2014, p. 864901.
Musmade KP, Trilok M, Dengale SJ, et al. Development and Validation of Liquid Chromatographic Method for Estimation of Naringin in Nanoformulation. J Pharm (Cairo). 2014;2014:864901.
Musmade, K. P., Trilok, M., Dengale, S. J., Bhat, K., Reddy, M. S., Musmade, P. B., & Udupa, N. (2014). Development and Validation of Liquid Chromatographic Method for Estimation of Naringin in Nanoformulation. Journal of Pharmaceutics, 2014, 864901. https://doi.org/10.1155/2014/864901
Musmade KP, et al. Development and Validation of Liquid Chromatographic Method for Estimation of Naringin in Nanoformulation. J Pharm (Cairo). 2014;2014:864901. PubMed PMID: 26556205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and Validation of Liquid Chromatographic Method for Estimation of Naringin in Nanoformulation. AU - Musmade,Kranti P, AU - Trilok,M, AU - Dengale,Swapnil J, AU - Bhat,Krishnamurthy, AU - Reddy,M S, AU - Musmade,Prashant B, AU - Udupa,N, Y1 - 2014/03/06/ PY - 2013/11/19/received PY - 2013/12/20/revised PY - 2013/12/26/accepted PY - 2015/11/12/entrez PY - 2014/1/1/pubmed PY - 2014/1/1/medline SP - 864901 EP - 864901 JF - Journal of pharmaceutics JO - J Pharm (Cairo) VL - 2014 N2 - A simple, precise, accurate, rapid, and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method with UV detection has been developed and validated for quantification of naringin (NAR) in novel pharmaceutical formulation. NAR is a polyphenolic flavonoid present in most of the citrus plants having variety of pharmacological activities. Method optimization was carried out by considering the various parameters such as effect of pH and column. The analyte was separated by employing a C18 (250.0 × 4.6 mm, 5 μm) column at ambient temperature in isocratic conditions using phosphate buffer pH 3.5: acetonitrile (75 : 25% v/v) as mobile phase pumped at a flow rate of 1.0 mL/min. UV detection was carried out at 282 nm. The developed method was validated according to ICH guidelines Q2(R1). The method was found to be precise and accurate on statistical evaluation with a linearity range of 0.1 to 20.0 μg/mL for NAR. The intra- and interday precision studies showed good reproducibility with coefficients of variation (CV) less than 1.0%. The mean recovery of NAR was found to be 99.33 ± 0.16%. The proposed method was found to be highly accurate, sensitive, and robust. The proposed liquid chromatographic method was successfully employed for the routine analysis of said compound in developed novel nanopharmaceuticals. The presence of excipients did not show any interference on the determination of NAR, indicating method specificity. SN - 2090-9918 UR - https://www.unboundmedicine.com/medline/citation/26556205/Development_and_Validation_of_Liquid_Chromatographic_Method_for_Estimation_of_Naringin_in_Nanoformulation_ L2 - https://dx.doi.org/10.1155/2014/864901 DB - PRIME DP - Unbound Medicine ER -
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