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Genome analysis of orf virus isolates from goats in the Fujian Province of southern China.
Front Microbiol 2015; 6:1135FM

Abstract

Orf virus (ORFV), a species of the genus Parapoxvirus of the family Poxviridae, causes non-systemic, highly contagious, and eruptive disease in sheep, goat, and other wild and domestic ruminants. Our previous work shows orf to be ubiquitous in the Fujian Province of China, a region where there is considerable heterogeneity among ORFVs. In this study, we sequenced full genomes of four Fujian goat ORFV strains (OV-GO, OV-YX, OV-NP, and OV-SJ1). The four strains were 132-139 kb in length, with each containing 124-132 genes and about 64% G+C content. The most notable differences between the four strains were found near the genome termini. OV-NP lacked seven and OV-SJ1 lacked three genes near the right terminus when compared against other ORFVs. We also investigated the skin-virulence of the four Fujian ORFVs in goats. The ORFVs with gene deletions showed low virulence while the ORFVs without gene deletions showed high virulence in goats suggesting gene deletion possibly leads to attenuation of ORFVs. Gene 134 was disrupted in OV-NP genome due to the lack of initial code. The phylogenetic tree based on complete Parapoxviruse genomes showed that sheep originated and goat originated ORFVs formed distinctly separate branches with 100% bootstrap. Based on the single gene phylogenetic tree of 132 genes of ORFVs, 47 genes can be easily distinguished as having originated from sheep or goats. In order to further reveal genetic variation presented in goat ORFVs and sheep ORFVs, we analyzed the deduced amino acid sequences of gene 008, multiple alignment of amino acid sequences of gene 008 from the genome of five goat ORFVs and four sheep ORFVs revealed 33 unique amino acids differentiating it as having sheep or goats as host. The availability of genomic sequences of four Fujian goat ORFVs aids in our understanding of the diversity of orf virus isolates in this region and can assist in distinguishing between orf strains that originate in sheep and goats.

Authors+Show Affiliations

College of Life Sciences, Fujian Agriculture and Forestry University Fuzhou, China ; University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University Fuzhou, China.College of Life Sciences, Fujian Agriculture and Forestry University Fuzhou, China ; University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University Fuzhou, China.Department of Immunology, School of Biotechnology, Southern Medical University Guangzhou, China.Department of Immunology, School of Biotechnology, Southern Medical University Guangzhou, China.Department of Immunology, School of Biotechnology, Southern Medical University Guangzhou, China.University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University Fuzhou, China.University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University Fuzhou, China.Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Champaign-Urbana Urbana, IL, USA.Department of Immunology, School of Biotechnology, Southern Medical University Guangzhou, China.College of Life Sciences, Fujian Agriculture and Forestry University Fuzhou, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26557108

Citation

Chi, Xuelin, et al. "Genome Analysis of Orf Virus Isolates From Goats in the Fujian Province of Southern China." Frontiers in Microbiology, vol. 6, 2015, p. 1135.
Chi X, Zeng X, Li W, et al. Genome analysis of orf virus isolates from goats in the Fujian Province of southern China. Front Microbiol. 2015;6:1135.
Chi, X., Zeng, X., Li, W., Hao, W., Li, M., Huang, X., ... Wang, S. (2015). Genome analysis of orf virus isolates from goats in the Fujian Province of southern China. Frontiers in Microbiology, 6, p. 1135. doi:10.3389/fmicb.2015.01135.
Chi X, et al. Genome Analysis of Orf Virus Isolates From Goats in the Fujian Province of Southern China. Front Microbiol. 2015;6:1135. PubMed PMID: 26557108.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome analysis of orf virus isolates from goats in the Fujian Province of southern China. AU - Chi,Xuelin, AU - Zeng,Xiancheng, AU - Li,Wei, AU - Hao,Wenbo, AU - Li,Ming, AU - Huang,Xiaohong, AU - Huang,Yifan, AU - Rock,Daniel L, AU - Luo,Shuhong, AU - Wang,Shihua, Y1 - 2015/10/23/ PY - 2015/04/26/received PY - 2015/10/02/accepted PY - 2015/11/12/entrez PY - 2015/11/12/pubmed PY - 2015/11/12/medline KW - Parapoxvirus KW - gene deletion KW - gene variation KW - orf virus KW - phylogenetic tree SP - 1135 EP - 1135 JF - Frontiers in microbiology JO - Front Microbiol VL - 6 N2 - Orf virus (ORFV), a species of the genus Parapoxvirus of the family Poxviridae, causes non-systemic, highly contagious, and eruptive disease in sheep, goat, and other wild and domestic ruminants. Our previous work shows orf to be ubiquitous in the Fujian Province of China, a region where there is considerable heterogeneity among ORFVs. In this study, we sequenced full genomes of four Fujian goat ORFV strains (OV-GO, OV-YX, OV-NP, and OV-SJ1). The four strains were 132-139 kb in length, with each containing 124-132 genes and about 64% G+C content. The most notable differences between the four strains were found near the genome termini. OV-NP lacked seven and OV-SJ1 lacked three genes near the right terminus when compared against other ORFVs. We also investigated the skin-virulence of the four Fujian ORFVs in goats. The ORFVs with gene deletions showed low virulence while the ORFVs without gene deletions showed high virulence in goats suggesting gene deletion possibly leads to attenuation of ORFVs. Gene 134 was disrupted in OV-NP genome due to the lack of initial code. The phylogenetic tree based on complete Parapoxviruse genomes showed that sheep originated and goat originated ORFVs formed distinctly separate branches with 100% bootstrap. Based on the single gene phylogenetic tree of 132 genes of ORFVs, 47 genes can be easily distinguished as having originated from sheep or goats. In order to further reveal genetic variation presented in goat ORFVs and sheep ORFVs, we analyzed the deduced amino acid sequences of gene 008, multiple alignment of amino acid sequences of gene 008 from the genome of five goat ORFVs and four sheep ORFVs revealed 33 unique amino acids differentiating it as having sheep or goats as host. The availability of genomic sequences of four Fujian goat ORFVs aids in our understanding of the diversity of orf virus isolates in this region and can assist in distinguishing between orf strains that originate in sheep and goats. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/26557108/Genome_analysis_of_orf_virus_isolates_from_goats_in_the_Fujian_Province_of_southern_China_ L2 - https://dx.doi.org/10.3389/fmicb.2015.01135 DB - PRIME DP - Unbound Medicine ER -