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Separation of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside from hawthorn leaves extracts using macroporous resins.
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Dec 15; 1007:23-9.JC

Abstract

Vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside are the major flavonoids of hawthorn leaves. In this work, the adsorption and desorption characteristics of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside on seven macroporous resins were evaluated. Among the tested resins, the HPD-400 resin showed the best adsorption and desorption capacities. Adsorption isotherms were constructed for the HPD-400 resin and well fitted to Langmuir and Freundlich models. Dynamic adsorption and desorption tests were performed on column packed with the HPD-400 resin to optimize the chromatographic parameters. After one run treatment with the HPD-400 resin, the contents of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside in the product were increased 8.44-fold and 8.43-fold from 0.720% and 2.63% to 6.08% and 22.2% with recovery yields of 79.1% and 81.2%, respectively. These results show that the developed method is a promising basis for the large-scale purification of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside from hawthorn leaves and other plant materials.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Li H
Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China; School of Pharmacy, Binzhou Medical University, Yantai 264003, PR China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, PR China.
Liu Y
Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
Jin H
School of life sciences, Yantai University, Yantai 264005, PR China.
Liu S
Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
Fang S
Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
Wang C
School of Pharmacy, Binzhou Medical University, Yantai 264003, PR China. Electronic address: chunhuawang508@126.com.
Xia C
Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China. Electronic address: chxia@yic.ac.cn.

MeSH

AdsorptionApigeninChromatography, High Pressure LiquidCrataegusGlucosidesHydrogen-Ion ConcentrationIsoflavonesKineticsPlant LeavesThermodynamics

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26562804

Citation

Li, Hongjuan, et al. "Separation of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside From Hawthorn Leaves Extracts Using Macroporous Resins." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 1007, 2015, pp. 23-9.
Li H, Liu Y, Jin H, et al. Separation of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside from hawthorn leaves extracts using macroporous resins. J Chromatogr B Analyt Technol Biomed Life Sci. 2015;1007:23-9.
Li, H., Liu, Y., Jin, H., Liu, S., Fang, S., Wang, C., & Xia, C. (2015). Separation of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside from hawthorn leaves extracts using macroporous resins. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1007, 23-9. https://doi.org/10.1016/j.jchromb.2015.10.043
Li H, et al. Separation of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside From Hawthorn Leaves Extracts Using Macroporous Resins. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Dec 15;1007:23-9. PubMed PMID: 26562804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside from hawthorn leaves extracts using macroporous resins. AU - Li,Hongjuan, AU - Liu,Ying, AU - Jin,Haizhu, AU - Liu,Sujing, AU - Fang,Shengtao, AU - Wang,Chunhua, AU - Xia,Chuanhai, Y1 - 2015/10/30/ PY - 2015/08/07/received PY - 2015/10/23/revised PY - 2015/10/24/accepted PY - 2015/11/13/entrez PY - 2015/11/13/pubmed PY - 2016/6/25/medline KW - Hawthorn leaves KW - Macroporous resins KW - Purification KW - Vitexin-2″-O-rhamnoside KW - Vitexin-4″-O-glucoside SP - 23 EP - 9 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 1007 N2 - Vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside are the major flavonoids of hawthorn leaves. In this work, the adsorption and desorption characteristics of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside on seven macroporous resins were evaluated. Among the tested resins, the HPD-400 resin showed the best adsorption and desorption capacities. Adsorption isotherms were constructed for the HPD-400 resin and well fitted to Langmuir and Freundlich models. Dynamic adsorption and desorption tests were performed on column packed with the HPD-400 resin to optimize the chromatographic parameters. After one run treatment with the HPD-400 resin, the contents of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside in the product were increased 8.44-fold and 8.43-fold from 0.720% and 2.63% to 6.08% and 22.2% with recovery yields of 79.1% and 81.2%, respectively. These results show that the developed method is a promising basis for the large-scale purification of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside from hawthorn leaves and other plant materials. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/26562804/Separation_of_vitexin_4″_O_glucoside_and_vitexin_2″_O_rhamnoside_from_hawthorn_leaves_extracts_using_macroporous_resins_ DB - PRIME DP - Unbound Medicine ER -
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