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Prevalence and Phylogenetic Characterization of Enterovirus D68 in Pediatric Patients with Acute Respiratory Tract Infection in Thailand.
Jpn J Infect Dis. 2016 Sep 21; 69(5):426-30.JJ

Abstract

Enterovirus D68 (EV-D68) is associated with severe lower respiratory tract infection and neurological abnormalities including acute myelitis and cranial nerve dysfunction. To determine whether an increased incidence of EV-D68 occurs in Southeast Asia, we retrospectively tested specimens collected from Thai pediatric patients who were less than 5 years of age and presented with acute respiratory tract infections between 2012 and 2014. Reverse transcription-polymerase chain reaction and nucleotide sequencing of the 5'-UTR/VP2 region were used to identify EV-D68. We also examined the epidemiological pattern of EV-D68 since 2009, when it was first identified in Thailand, and compiled records of clinical manifestations in children with confirmed EV-D68 infection. From 837 samples, 5 samples (0.6%) tested positive for EV-D68. All patients presented with viral pneumonia and required hospitalization. Phylogenetic analysis of the VP4/VP2 regions revealed that EV-D68 strains circulating in Thailand between 2012 and 2014 were closely related to strains reported in Japan, United Kingdom, China, and France. Continued surveillance of probable EV-D68-associated severe respiratory tract infection and the development of a rapid diagnostic test for EV-D68 are essential in supporting awareness and facilitating disease prevention and control.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Thongpan I
Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University.
Wanlapakorn N
No affiliation info available
Vongpunsawad S
No affiliation info available
Linsuwanon P
No affiliation info available
Theamboonlers A
No affiliation info available
Payungporn S
No affiliation info available
Poovorawan Y
No affiliation info available

MeSH

Child, PreschoolEnterovirusEnterovirus InfectionsFemaleHumansInfantMaleMolecular EpidemiologyPhylogenyPrevalenceRespiratory Tract InfectionsRetrospective StudiesReverse Transcriptase Polymerase Chain ReactionSequence Analysis, DNASequence HomologyThailandViral Structural Proteins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26567839

Citation

Thongpan, Ilada, et al. "Prevalence and Phylogenetic Characterization of Enterovirus D68 in Pediatric Patients With Acute Respiratory Tract Infection in Thailand." Japanese Journal of Infectious Diseases, vol. 69, no. 5, 2016, pp. 426-30.
Thongpan I, Wanlapakorn N, Vongpunsawad S, et al. Prevalence and Phylogenetic Characterization of Enterovirus D68 in Pediatric Patients with Acute Respiratory Tract Infection in Thailand. Jpn J Infect Dis. 2016;69(5):426-30.
Thongpan, I., Wanlapakorn, N., Vongpunsawad, S., Linsuwanon, P., Theamboonlers, A., Payungporn, S., & Poovorawan, Y. (2016). Prevalence and Phylogenetic Characterization of Enterovirus D68 in Pediatric Patients with Acute Respiratory Tract Infection in Thailand. Japanese Journal of Infectious Diseases, 69(5), 426-30. https://doi.org/10.7883/yoken.JJID.2015.352
Thongpan I, et al. Prevalence and Phylogenetic Characterization of Enterovirus D68 in Pediatric Patients With Acute Respiratory Tract Infection in Thailand. Jpn J Infect Dis. 2016 Sep 21;69(5):426-30. PubMed PMID: 26567839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence and Phylogenetic Characterization of Enterovirus D68 in Pediatric Patients with Acute Respiratory Tract Infection in Thailand. AU - Thongpan,Ilada, AU - Wanlapakorn,Nasamon, AU - Vongpunsawad,Sompong, AU - Linsuwanon,Piyada, AU - Theamboonlers,Apiradee, AU - Payungporn,Sunchai, AU - Poovorawan,Yong, Y1 - 2015/11/13/ PY - 2015/11/17/entrez PY - 2015/11/17/pubmed PY - 2017/3/23/medline SP - 426 EP - 30 JF - Japanese journal of infectious diseases JO - Jpn J Infect Dis VL - 69 IS - 5 N2 - Enterovirus D68 (EV-D68) is associated with severe lower respiratory tract infection and neurological abnormalities including acute myelitis and cranial nerve dysfunction. To determine whether an increased incidence of EV-D68 occurs in Southeast Asia, we retrospectively tested specimens collected from Thai pediatric patients who were less than 5 years of age and presented with acute respiratory tract infections between 2012 and 2014. Reverse transcription-polymerase chain reaction and nucleotide sequencing of the 5'-UTR/VP2 region were used to identify EV-D68. We also examined the epidemiological pattern of EV-D68 since 2009, when it was first identified in Thailand, and compiled records of clinical manifestations in children with confirmed EV-D68 infection. From 837 samples, 5 samples (0.6%) tested positive for EV-D68. All patients presented with viral pneumonia and required hospitalization. Phylogenetic analysis of the VP4/VP2 regions revealed that EV-D68 strains circulating in Thailand between 2012 and 2014 were closely related to strains reported in Japan, United Kingdom, China, and France. Continued surveillance of probable EV-D68-associated severe respiratory tract infection and the development of a rapid diagnostic test for EV-D68 are essential in supporting awareness and facilitating disease prevention and control. SN - 1884-2836 UR - https://www.unboundmedicine.com/medline/citation/26567839/Prevalence_and_Phylogenetic_Characterization_of_Enterovirus_D68_in_Pediatric_Patients_with_Acute_Respiratory_Tract_Infection_in_Thailand_ DB - PRIME DP - Unbound Medicine ER -