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Sperm parameters, protamine deficiency, and apoptosis in total globozoospermia.
Iran J Reprod Med. 2015 Aug; 13(8):495-502.IJ

Abstract

BACKGROUND

Globozoospermia is a severe form of teratozoospermia (incidence < 0.1%) in infertile men that is characterized by round headed sperm and acrosomeless in semen.

OBJECTIVE

To compare the semen parameters, protamine deficiency, and apoptosis in ejaculated spermatozoa between globozoospermic and normozoospermic men.

MATERIALS AND METHODS

Thirty six semen samples were divided into two groups including 15 infertile men with total globozoospermic (> 90% round-headed sperm) and 21 healthy donors with normal spermograms as controls. Semen analysis was performed according to World Health Organization criteria (2010). Sperm protamine deficiency was assessed using Chromomycin A3 (CMA3) staining and the rate of apoptotic spermatozoa was evaluated with TUNEL assay.

RESULTS

Sperm concentration, motility, and normal morphology in globozoospermic men were significantly decreased compared with controls (p<0.05). The rate of CMA3-reacted spermatozoa (CMA3+) in globozoospermic men was higher than controls (65.93 ± 11.77 vs. 21.24 ± 7.37, respectively, p<0.0001). The rate of apoptotic spermatozoa (TUNEL positive) were significantly increased in globozoospermic cases with respect to the controls (17.60 ± 10.72 and 5.95 ± 3.02, respectively, p<0.0001). There was no significant correlation between sperm protamine deficiency and apoptosis in globozoospermic men.

CONCLUSION

Globozoospermic samples contain a higher proportion of spermatozoa with abnormal chromatin packaging and DNA fragmentation than normozoospermic samples. Therefore, in addition to absence of acrosome in the spermatozoa of globozoospermic patients, the high percentage of spermatozoa with immature chromatin and apoptotic marker may be considered as the other etiologies of infertility in these patients.

Authors+Show Affiliations

International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26568752

Citation

Ghasemzadeh, Jalal, et al. "Sperm Parameters, Protamine Deficiency, and Apoptosis in Total Globozoospermia." Iranian Journal of Reproductive Medicine, vol. 13, no. 8, 2015, pp. 495-502.
Ghasemzadeh J, Talebi AR, Khalili MA, et al. Sperm parameters, protamine deficiency, and apoptosis in total globozoospermia. Iran J Reprod Med. 2015;13(8):495-502.
Ghasemzadeh, J., Talebi, A. R., Khalili, M. A., Fesahat, F., Halvaei, I., Nabi, A., & Ashourzadeh, S. (2015). Sperm parameters, protamine deficiency, and apoptosis in total globozoospermia. Iranian Journal of Reproductive Medicine, 13(8), 495-502.
Ghasemzadeh J, et al. Sperm Parameters, Protamine Deficiency, and Apoptosis in Total Globozoospermia. Iran J Reprod Med. 2015;13(8):495-502. PubMed PMID: 26568752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sperm parameters, protamine deficiency, and apoptosis in total globozoospermia. AU - Ghasemzadeh,Jalal, AU - Talebi,Ali Reza, AU - Khalili,Mohammad Ali, AU - Fesahat,Farzaneh, AU - Halvaei,Iman, AU - Nabi,Ali, AU - Ashourzadeh,Sareh, PY - 2015/11/17/entrez PY - 2015/11/17/pubmed PY - 2015/11/17/medline KW - Apoptosis KW - DNA damage KW - Male infertility KW - Sperm protamine SP - 495 EP - 502 JF - Iranian journal of reproductive medicine JO - Iran J Reprod Med VL - 13 IS - 8 N2 - BACKGROUND: Globozoospermia is a severe form of teratozoospermia (incidence < 0.1%) in infertile men that is characterized by round headed sperm and acrosomeless in semen. OBJECTIVE: To compare the semen parameters, protamine deficiency, and apoptosis in ejaculated spermatozoa between globozoospermic and normozoospermic men. MATERIALS AND METHODS: Thirty six semen samples were divided into two groups including 15 infertile men with total globozoospermic (> 90% round-headed sperm) and 21 healthy donors with normal spermograms as controls. Semen analysis was performed according to World Health Organization criteria (2010). Sperm protamine deficiency was assessed using Chromomycin A3 (CMA3) staining and the rate of apoptotic spermatozoa was evaluated with TUNEL assay. RESULTS: Sperm concentration, motility, and normal morphology in globozoospermic men were significantly decreased compared with controls (p<0.05). The rate of CMA3-reacted spermatozoa (CMA3+) in globozoospermic men was higher than controls (65.93 ± 11.77 vs. 21.24 ± 7.37, respectively, p<0.0001). The rate of apoptotic spermatozoa (TUNEL positive) were significantly increased in globozoospermic cases with respect to the controls (17.60 ± 10.72 and 5.95 ± 3.02, respectively, p<0.0001). There was no significant correlation between sperm protamine deficiency and apoptosis in globozoospermic men. CONCLUSION: Globozoospermic samples contain a higher proportion of spermatozoa with abnormal chromatin packaging and DNA fragmentation than normozoospermic samples. Therefore, in addition to absence of acrosome in the spermatozoa of globozoospermic patients, the high percentage of spermatozoa with immature chromatin and apoptotic marker may be considered as the other etiologies of infertility in these patients. SN - 1680-6433 UR - https://www.unboundmedicine.com/medline/citation/26568752/Sperm_parameters_protamine_deficiency_and_apoptosis_in_total_globozoospermia_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26568752/ DB - PRIME DP - Unbound Medicine ER -
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