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Three-dimensional cellulose sponge: Fabrication, characterization, biomimetic mineralization, and in vitro cell infiltration.
Carbohydr Polym 2016; 136:154-62CP

Abstract

In this study, cellulose based scaffolds were produced by electrospinning of cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h. The resulting nonwoven cellulose mat was treated with sodium borohydride (SB) solution. In situ hydrolysis of SB solution into the pores of the membrane produced hydrogen gas resulting a three-dimensional (3D) cellulose sponge. SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane. 3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution. SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone. 3D cellulose sponge exhibited the better cell infiltration, spreading and proliferation compared to 2D cellulose mat. Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications.

Authors+Show Affiliations

Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea; Department of Chemistry, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal.Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea; Research Center for Next Generation, Kalanki, Kathmandu, Nepal.Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea.Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea.Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea.Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea; Department of Convergence Technology Engineering, College of Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea.Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: biochan@jbnu.ac.kr.Department of Bionanosystem Engineering, Graduate School, Chonbuk National University, Jeonju 561-756, Republic of Korea; Division of Mechanical Design Engineering, School of Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Eco-friendly Machine Parts Design Research Center, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: chskim@jbnu.ac.kr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26572341

Citation

Joshi, Mahesh Kumar, et al. "Three-dimensional Cellulose Sponge: Fabrication, Characterization, Biomimetic Mineralization, and in Vitro Cell Infiltration." Carbohydrate Polymers, vol. 136, 2016, pp. 154-62.
Joshi MK, Pant HR, Tiwari AP, et al. Three-dimensional cellulose sponge: Fabrication, characterization, biomimetic mineralization, and in vitro cell infiltration. Carbohydr Polym. 2016;136:154-62.
Joshi, M. K., Pant, H. R., Tiwari, A. P., Maharjan, B., Liao, N., Kim, H. J., ... Kim, C. S. (2016). Three-dimensional cellulose sponge: Fabrication, characterization, biomimetic mineralization, and in vitro cell infiltration. Carbohydrate Polymers, 136, pp. 154-62. doi:10.1016/j.carbpol.2015.09.018.
Joshi MK, et al. Three-dimensional Cellulose Sponge: Fabrication, Characterization, Biomimetic Mineralization, and in Vitro Cell Infiltration. Carbohydr Polym. 2016 Jan 20;136:154-62. PubMed PMID: 26572341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Three-dimensional cellulose sponge: Fabrication, characterization, biomimetic mineralization, and in vitro cell infiltration. AU - Joshi,Mahesh Kumar, AU - Pant,Hem Raj, AU - Tiwari,Arjun Prasad, AU - Maharjan,Bikendra, AU - Liao,Nina, AU - Kim,Han Joo, AU - Park,Chan Hee, AU - Kim,Cheol Sang, Y1 - 2015/09/16/ PY - 2015/04/28/received PY - 2015/09/06/revised PY - 2015/09/07/accepted PY - 2015/11/18/entrez PY - 2015/11/18/pubmed PY - 2016/9/13/medline KW - 3D scaffold KW - Biomimetic mineralization KW - Cellulose KW - Saponification SP - 154 EP - 62 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 136 N2 - In this study, cellulose based scaffolds were produced by electrospinning of cellulose acetate (CA) solution followed by its saponification with NaOH/ethanol system for 24h. The resulting nonwoven cellulose mat was treated with sodium borohydride (SB) solution. In situ hydrolysis of SB solution into the pores of the membrane produced hydrogen gas resulting a three-dimensional (3D) cellulose sponge. SEM images demonstrated an open porous and loosely packed fibrous mesh compared to the tightly packed single-layered structure of the conventional electrospun membrane. 3D cellulose sponge showed admirable ability to nucleate bioactive calcium phosphate (Ca-P) crystals in simulated body fluid (SBF) solution. SEM-EDX and X-ray diffraction studies revealed that the minerals deposited on the nanofibers have the nonstoichiometric composition similar to that of hydroxyapatite, the mineralized component of the bone. 3D cellulose sponge exhibited the better cell infiltration, spreading and proliferation compared to 2D cellulose mat. Therefore, a facile fabrication of 3D cellulose sponge with improved mineralization represents an innovative strategy for the bone tissue engineering applications. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/26572341/Three_dimensional_cellulose_sponge:_Fabrication_characterization_biomimetic_mineralization_and_in_vitro_cell_infiltration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0144-8617(15)00865-6 DB - PRIME DP - Unbound Medicine ER -