Tags

Type your tag names separated by a space and hit enter

Impact of combined β-glucanase and xylanase enzymes on growth performance, nutrients utilization and gut microbiota in broiler chickens fed corn or wheat-based diets.
Poult Sci 2016; 95(3):528-40PS

Abstract

The effects of a xylanase and β-glucanase (XB) blend (2,500 U of xylanase and 250 U of β-glucanase per kg of complete feed) on growth performance, nutrients utilization and digesta microbiota in broiler chickens were investigated. A total of 140 day-old male Ross 308 broiler chicks were randomly assigned to 7 replicate cages and fed experimental diets. Diets were based on either corn or wheat without or with supplemental XB. Performance was monitored weekly and excreta were collected from d 17 to 20 for nutrients digestibility and AMEn measurements. On d 21, jejunal contents were collected for viscosity determination whereas ileal and cecal contents were obtained for microbial analysis by Illumina sequencing. Microbial data were analyzed using QIIME and PLS-DA whilst other data were analyzed using SAS. Birds fed wheat diets had higher (P < 0.001) BWG (3.4%) than birds fed corn-based diet whilst birds fed XB had better BWG (4%) and FCR (7%) than birds fed non-XB diets. Birds fed wheat diet had higher (P < 0.001) NDF (46.5%) and less (P = 0.01) CP (-5.4%) digestibility compared to birds fed corn-based diet. XB reduced (P < 0.001) jejunal digesta viscosity to a greater extent in wheat diet (-31%) than in corn-based diet (-10%). Birds fed wheat-based diet with XB had higher (3.5%) starch digestibility than birds fed this diet without XB. Janthinobacterium was associated with non-XB corn-based diet, whereas Ruminococcus, Lachnospiraceae, Lactobacillaceae, Peptostreptococcaceae, Clostridiales, Acidovorax and Blautia were associated with XB corn-based diet in the ileum. A relatively similar microbiome clustering was observed in wheat-based treatments in the cecum. There were no significant (P ≥ 0.05) correlations between selected ileal or cecal bacterial taxa and AMEn. Diet impacted growth performance but XB was efficacious across diet types, implying that degradation of dietary fibrous components by feed enzymes may stimulate performance in young birds. Data provided significant insight on ileal and cecal microbial profile associated with the dietary types and XB; however their functional roles require further investigations.

Authors+Show Affiliations

Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada R3T 2N2.Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada R3T 2N2.Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada R3T 2N2 DuPont Industrial Biosciences-Danisco Animal Nutrition, Marlborough, United Kingdom SN8 Department of Animal Biosciences, University of Guelph, Guelph, ON, N1G 2W1 ekiarie@uoguelph.ca.Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada R3T 2N2.Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada R3T 2N2.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26574039

Citation

Munyaka, P M., et al. "Impact of Combined Β-glucanase and Xylanase Enzymes On Growth Performance, Nutrients Utilization and Gut Microbiota in Broiler Chickens Fed Corn or Wheat-based Diets." Poultry Science, vol. 95, no. 3, 2016, pp. 528-40.
Munyaka PM, Nandha NK, Kiarie E, et al. Impact of combined β-glucanase and xylanase enzymes on growth performance, nutrients utilization and gut microbiota in broiler chickens fed corn or wheat-based diets. Poult Sci. 2016;95(3):528-40.
Munyaka, P. M., Nandha, N. K., Kiarie, E., Nyachoti, C. M., & Khafipour, E. (2016). Impact of combined β-glucanase and xylanase enzymes on growth performance, nutrients utilization and gut microbiota in broiler chickens fed corn or wheat-based diets. Poultry Science, 95(3), pp. 528-40. doi:10.3382/ps/pev333.
Munyaka PM, et al. Impact of Combined Β-glucanase and Xylanase Enzymes On Growth Performance, Nutrients Utilization and Gut Microbiota in Broiler Chickens Fed Corn or Wheat-based Diets. Poult Sci. 2016;95(3):528-40. PubMed PMID: 26574039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of combined β-glucanase and xylanase enzymes on growth performance, nutrients utilization and gut microbiota in broiler chickens fed corn or wheat-based diets. AU - Munyaka,P M, AU - Nandha,N K, AU - Kiarie,E, AU - Nyachoti,C M, AU - Khafipour,E, Y1 - 2015/11/14/ PY - 2015/09/03/accepted PY - 2015/05/14/received PY - 2015/11/18/entrez PY - 2015/11/18/pubmed PY - 2016/5/4/medline KW - Broiler KW - microbiota KW - performance KW - xylanase KW - β-glucanase SP - 528 EP - 40 JF - Poultry science JO - Poult. Sci. VL - 95 IS - 3 N2 - The effects of a xylanase and β-glucanase (XB) blend (2,500 U of xylanase and 250 U of β-glucanase per kg of complete feed) on growth performance, nutrients utilization and digesta microbiota in broiler chickens were investigated. A total of 140 day-old male Ross 308 broiler chicks were randomly assigned to 7 replicate cages and fed experimental diets. Diets were based on either corn or wheat without or with supplemental XB. Performance was monitored weekly and excreta were collected from d 17 to 20 for nutrients digestibility and AMEn measurements. On d 21, jejunal contents were collected for viscosity determination whereas ileal and cecal contents were obtained for microbial analysis by Illumina sequencing. Microbial data were analyzed using QIIME and PLS-DA whilst other data were analyzed using SAS. Birds fed wheat diets had higher (P < 0.001) BWG (3.4%) than birds fed corn-based diet whilst birds fed XB had better BWG (4%) and FCR (7%) than birds fed non-XB diets. Birds fed wheat diet had higher (P < 0.001) NDF (46.5%) and less (P = 0.01) CP (-5.4%) digestibility compared to birds fed corn-based diet. XB reduced (P < 0.001) jejunal digesta viscosity to a greater extent in wheat diet (-31%) than in corn-based diet (-10%). Birds fed wheat-based diet with XB had higher (3.5%) starch digestibility than birds fed this diet without XB. Janthinobacterium was associated with non-XB corn-based diet, whereas Ruminococcus, Lachnospiraceae, Lactobacillaceae, Peptostreptococcaceae, Clostridiales, Acidovorax and Blautia were associated with XB corn-based diet in the ileum. A relatively similar microbiome clustering was observed in wheat-based treatments in the cecum. There were no significant (P ≥ 0.05) correlations between selected ileal or cecal bacterial taxa and AMEn. Diet impacted growth performance but XB was efficacious across diet types, implying that degradation of dietary fibrous components by feed enzymes may stimulate performance in young birds. Data provided significant insight on ileal and cecal microbial profile associated with the dietary types and XB; however their functional roles require further investigations. SN - 0032-5791 UR - https://www.unboundmedicine.com/medline/citation/26574039/Impact_of_combined_β_glucanase_and_xylanase_enzymes_on_growth_performance_nutrients_utilization_and_gut_microbiota_in_broiler_chickens_fed_corn_or_wheat_based_diets_ L2 - https://academic.oup.com/ps/article-lookup/doi/10.3382/ps/pev333 DB - PRIME DP - Unbound Medicine ER -