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Characterization of Insulin-Immunoreactive Cells and Endocrine Cells Within the Duct System of the Adult Human Pancreas.
Pancreas. 2016 May-Jun; 45(5):735-42.P

Abstract

OBJECTIVE

The adult pancreatic duct system accommodates endocrine cells that have the potential to produce insulin. Here we report the characterization and distribution of insulin-immunoreactive cells and endocrine cells within the ductal units of adult human pancreas.

METHODS

Sequential pancreas sections from 12 nondiabetic adults were stained with biomarkers of ductal epithelial cells (cytokeratin 19), acinar cells (amylase), endocrine cells (chromogranin A; neuron-specific enolase), islet hormones (insulin, glucagon, somatostatin, pancreatic polypeptide), cell proliferation (Ki-67), and neogenesis (CD29).

RESULTS

The number of islet hormone-immunoreactive cells increased from large ducts to the terminal branches. The insulin-producing cells outnumbered endocrine cells reactive for glucagon, somatostatin, or pancreatic polypeptide. The proportions of insulin-immunoreactive count compared with local islets (100% as a baseline) were 1.5% for the main ducts, 7.2% for interlobular ducts, 24.8% for intralobular ducts, 67.9% for intercalated ducts, and 348.9% for centroacinar cells. Both Ki-67- and CD29-labeled cells were predominantly localized in the terminal branches around the islets. The terminal branches also showed cells coexpressing islet hormones and cytokeratin 19.

CONCLUSIONS

The adult human pancreatic ducts showed islet hormone-producing cells. The insulin-reactive cells predominantly localized in terminal branches where they may retain potential capability for β-cell neogenesis.

Authors+Show Affiliations

From the *Center for Diabetic Systems Medicine, Faculty of Basic Medicine, Guilin Medical University, Guilin; and †Molecular Endocrinology and Toxicology Laboratory, Department of Biology, Hong Kong Baptist University, Hong Kong, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26580455

Citation

Li, Rong, et al. "Characterization of Insulin-Immunoreactive Cells and Endocrine Cells Within the Duct System of the Adult Human Pancreas." Pancreas, vol. 45, no. 5, 2016, pp. 735-42.
Li R, Zhang X, Yu L, et al. Characterization of Insulin-Immunoreactive Cells and Endocrine Cells Within the Duct System of the Adult Human Pancreas. Pancreas. 2016;45(5):735-42.
Li, R., Zhang, X., Yu, L., Zou, X., & Zhao, H. (2016). Characterization of Insulin-Immunoreactive Cells and Endocrine Cells Within the Duct System of the Adult Human Pancreas. Pancreas, 45(5), 735-42. https://doi.org/10.1097/MPA.0000000000000555
Li R, et al. Characterization of Insulin-Immunoreactive Cells and Endocrine Cells Within the Duct System of the Adult Human Pancreas. Pancreas. 2016 May-Jun;45(5):735-42. PubMed PMID: 26580455.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Insulin-Immunoreactive Cells and Endocrine Cells Within the Duct System of the Adult Human Pancreas. AU - Li,Rong, AU - Zhang,Xiaoxi, AU - Yu,Lan, AU - Zou,Xia, AU - Zhao,Hailu, PY - 2015/11/19/entrez PY - 2015/11/19/pubmed PY - 2017/1/12/medline SP - 735 EP - 42 JF - Pancreas JO - Pancreas VL - 45 IS - 5 N2 - OBJECTIVE: The adult pancreatic duct system accommodates endocrine cells that have the potential to produce insulin. Here we report the characterization and distribution of insulin-immunoreactive cells and endocrine cells within the ductal units of adult human pancreas. METHODS: Sequential pancreas sections from 12 nondiabetic adults were stained with biomarkers of ductal epithelial cells (cytokeratin 19), acinar cells (amylase), endocrine cells (chromogranin A; neuron-specific enolase), islet hormones (insulin, glucagon, somatostatin, pancreatic polypeptide), cell proliferation (Ki-67), and neogenesis (CD29). RESULTS: The number of islet hormone-immunoreactive cells increased from large ducts to the terminal branches. The insulin-producing cells outnumbered endocrine cells reactive for glucagon, somatostatin, or pancreatic polypeptide. The proportions of insulin-immunoreactive count compared with local islets (100% as a baseline) were 1.5% for the main ducts, 7.2% for interlobular ducts, 24.8% for intralobular ducts, 67.9% for intercalated ducts, and 348.9% for centroacinar cells. Both Ki-67- and CD29-labeled cells were predominantly localized in the terminal branches around the islets. The terminal branches also showed cells coexpressing islet hormones and cytokeratin 19. CONCLUSIONS: The adult human pancreatic ducts showed islet hormone-producing cells. The insulin-reactive cells predominantly localized in terminal branches where they may retain potential capability for β-cell neogenesis. SN - 1536-4828 UR - https://www.unboundmedicine.com/medline/citation/26580455/Characterization_of_Insulin_Immunoreactive_Cells_and_Endocrine_Cells_Within_the_Duct_System_of_the_Adult_Human_Pancreas_ L2 - https://doi.org/10.1097/MPA.0000000000000555 DB - PRIME DP - Unbound Medicine ER -