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Development and validation of an LC/MS/MS method for simultaneous determination of shionone and epi-friedelinol in rat plasma for pharmacokinetic study after oral administration of Aster tataricus extract.
Biomed Chromatogr. 2016 Jul; 30(7):1112-1117.BC

Abstract

A rapid and sensitive liquid chromatography tandem mass spectrometry (LC/MS/MS) method was developed and validated using spinasterol as the internal standard (IS) for the simultaneous determination of shionone and epi-friedelinol in rat plasma. Plasma samples were pretreated using liquid-liquid extraction with ethyl ether. Chromatographic separation was achieved on a C18 column (100 × 2.1 mm, 5 μm) with an isocratic elution consisting of acetonitrile-0.1% formic acid water (75:25, v/v) at a flow rate of 0.30 mL/min. Detection was performed under the selected reaction monitoring scan using an electrospray ionization in the positive ion mode. The mass transitions were as follows: m/z 427.4 → 95.1 for shionone, m/z 411.4 → 205.2 for epi-friedelinol and m/z 395.3 → 105.2 for IS. All calibration curves exhibited good linearity (r > 0.995) over the concentration range for both components. The intra- and inter-day precisions at three QC and lower limit of quantitation levels were both <10.21% in terms of relative standard deviation, and the accuracy ranged from -7.13 to 8.02% in terms of relative error. The extraction recoveries of the compounds ranged from 82.07 to 89.81%. The developed method was successfully applied to the pharmacokinetic study of shionone and epi-friedelinol after oral administration of Aster tataricus extract to rats. Copyright © 2015 John Wiley & Sons, Ltd.

Authors+Show Affiliations

Department of Emergency Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.Department of Emergency Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.Department of Emergency Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.Department of Emergency Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.Department of Emergency Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.Department of Respiratory Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.Department of Respiratory Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.Department of Respiratory Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.Department of Emergency Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, China.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

26581126

Citation

Yin, De-Feng, et al. "Development and Validation of an LC/MS/MS Method for Simultaneous Determination of Shionone and Epi-friedelinol in Rat Plasma for Pharmacokinetic Study After Oral Administration of Aster Tataricus Extract." Biomedical Chromatography : BMC, vol. 30, no. 7, 2016, pp. 1112-1117.
Yin DF, Zhou K, Liu JT, et al. Development and validation of an LC/MS/MS method for simultaneous determination of shionone and epi-friedelinol in rat plasma for pharmacokinetic study after oral administration of Aster tataricus extract. Biomed Chromatogr. 2016;30(7):1112-1117.
Yin, D. F., Zhou, K., Liu, J. T., Hu, L., Liu, Y., Deng, J., Wang, S. P., Xiong, Y., & Zhong, W. (2016). Development and validation of an LC/MS/MS method for simultaneous determination of shionone and epi-friedelinol in rat plasma for pharmacokinetic study after oral administration of Aster tataricus extract. Biomedical Chromatography : BMC, 30(7), 1112-1117. https://doi.org/10.1002/bmc.3658
Yin DF, et al. Development and Validation of an LC/MS/MS Method for Simultaneous Determination of Shionone and Epi-friedelinol in Rat Plasma for Pharmacokinetic Study After Oral Administration of Aster Tataricus Extract. Biomed Chromatogr. 2016;30(7):1112-1117. PubMed PMID: 26581126.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of an LC/MS/MS method for simultaneous determination of shionone and epi-friedelinol in rat plasma for pharmacokinetic study after oral administration of Aster tataricus extract. AU - Yin,De-Feng, AU - Zhou,Kai, AU - Liu,Ji-Tao, AU - Hu,Li, AU - Liu,Ying, AU - Deng,Jun, AU - Wang,Song-Ping, AU - Xiong,Ying, AU - Zhong,Wu, Y1 - 2015/12/20/ PY - 2015/07/13/received PY - 2015/10/07/revised PY - 2015/11/10/accepted PY - 2015/11/20/entrez PY - 2015/11/20/pubmed PY - 2017/1/25/medline KW - aster tataricus extract KW - epi-friedelinol KW - pharmacokinetic study KW - shionone SP - 1112 EP - 1117 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 30 IS - 7 N2 - A rapid and sensitive liquid chromatography tandem mass spectrometry (LC/MS/MS) method was developed and validated using spinasterol as the internal standard (IS) for the simultaneous determination of shionone and epi-friedelinol in rat plasma. Plasma samples were pretreated using liquid-liquid extraction with ethyl ether. Chromatographic separation was achieved on a C18 column (100 × 2.1 mm, 5 μm) with an isocratic elution consisting of acetonitrile-0.1% formic acid water (75:25, v/v) at a flow rate of 0.30 mL/min. Detection was performed under the selected reaction monitoring scan using an electrospray ionization in the positive ion mode. The mass transitions were as follows: m/z 427.4 → 95.1 for shionone, m/z 411.4 → 205.2 for epi-friedelinol and m/z 395.3 → 105.2 for IS. All calibration curves exhibited good linearity (r > 0.995) over the concentration range for both components. The intra- and inter-day precisions at three QC and lower limit of quantitation levels were both <10.21% in terms of relative standard deviation, and the accuracy ranged from -7.13 to 8.02% in terms of relative error. The extraction recoveries of the compounds ranged from 82.07 to 89.81%. The developed method was successfully applied to the pharmacokinetic study of shionone and epi-friedelinol after oral administration of Aster tataricus extract to rats. Copyright © 2015 John Wiley & Sons, Ltd. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/26581126/Development_and_validation_of_an_LC/MS/MS_method_for_simultaneous_determination_of_shionone_and_epi_friedelinol_in_rat_plasma_for_pharmacokinetic_study_after_oral_administration_of_Aster_tataricus_extract_ L2 - https://doi.org/10.1002/bmc.3658 DB - PRIME DP - Unbound Medicine ER -