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Comparative study of the modulation of fructose/sucrose-induced hepatic steatosis by mixed lipid formulations varying in unsaturated fatty acid content.
Nutr Metab (Lond) 2015; 12:41NM

Abstract

BACKGROUND

Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease in developed countries. NAFLD encompasses a spectrum of diseases, ranging from hepatic steatosis to non-alcoholic steatohepatitis (NASH), cirrhosis, and liver failure. The etiology of NAFLD remains unclear but is thought to relate to increased fatty acid flux within the liver that results in toxic fatty acid metabolite production. One source of increased fatty acid flux is fructose/sucrose-induced hepatic lipogenesis. Current treatment for NAFLD encompasses dietary modifications. However, little scientific evidence exists on which to base many dietary recommendations, especially the intake of different types of carbohydrates and fats. We hypothesized that lipid mixtures of unsaturated fatty acids would inhibit lipogenesis and subsequent hepatic steatosis induced by high carbohydrate diets. The aim of this study was to examine the effects of different complex mixtures of fatty acids upon the development of fructose/sucrose-induced hepatic steatosis.

METHODS

C57BL/6 mice were randomized to normocaloric chow-based diets that varied in the type of carbohydrate (starch, sucrose, fructose). Animals in each carbohydrate group were further randomized to diets that varied in lipid type (no additional lipid, soybean oil, fish oil, olive/soybean oil, macadamia nut oil). These oils were chosen based upon their content of omega-6 polyunsaturated fatty acids, omega-3 polyunsaturated fatty acids, omega-9 monounsaturated fatty acids, or omega-7 monounsaturated fatty acids. Fatty acid flux in the liver was determine by assessing hepatic lipid content (steatosis). We also assessed fatty acid levels in the plasma and liver of the animals, hepatic lipogenesis activity, hepatic stearoyl-CoA-1 desaturase activity, and hepatic elongase activity.

RESULTS

Animals consumed similar amounts of the diets and maintained normal body weights throughout the study. Both sucrose and fructose induced hepatic lipogenesis and steatosis, with fructose being more potent. All mixed lipids similarly inhibited steatosis, limiting lipid content to levels found in the control (starch) animals. Lipogenesis and stearoyl-CoA-1 desaturase activity were increased in the sucrose and fructose groups. Levels of these enzymatic processes remained at baseline in all of the lipid groups.

CONCLUSION

This is the first study to compare various complex lipid mixtures, based upon dietary oils with different types of long-chain fatty acids, upon development of sucrose/fructose-induced steatosis. Both carbohydrate source and lipid content appear important for the modulation of steatosis. Moderate intake of complex lipids with high unsaturated to saturated fatty acid ratios inhibited both lipogenesis and steatosis.

Authors+Show Affiliations

Methodist Research Institute, Indiana University Health, 1800 N. Capitol Ave, Indianapolis, IN 46202 USA.Methodist Research Institute, Indiana University Health, 1800 N. Capitol Ave, Indianapolis, IN 46202 USA.Methodist Research Institute, Indiana University Health, 1800 N. Capitol Ave, Indianapolis, IN 46202 USA.Baxter Healthcare Corporation, Deerfield, IL 60015 USA.Baxter Healthcare Corporation, Deerfield, IL 60015 USA.Baxter Healthcare Corporation, Deerfield, IL 60015 USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26583036

Citation

Siddiqui, Rafat A., et al. "Comparative Study of the Modulation of Fructose/sucrose-induced Hepatic Steatosis By Mixed Lipid Formulations Varying in Unsaturated Fatty Acid Content." Nutrition & Metabolism, vol. 12, 2015, p. 41.
Siddiqui RA, Xu Z, Harvey KA, et al. Comparative study of the modulation of fructose/sucrose-induced hepatic steatosis by mixed lipid formulations varying in unsaturated fatty acid content. Nutr Metab (Lond). 2015;12:41.
Siddiqui, R. A., Xu, Z., Harvey, K. A., Pavlina, T. M., Becker, M. J., & Zaloga, G. P. (2015). Comparative study of the modulation of fructose/sucrose-induced hepatic steatosis by mixed lipid formulations varying in unsaturated fatty acid content. Nutrition & Metabolism, 12, p. 41. doi:10.1186/s12986-015-0038-x.
Siddiqui RA, et al. Comparative Study of the Modulation of Fructose/sucrose-induced Hepatic Steatosis By Mixed Lipid Formulations Varying in Unsaturated Fatty Acid Content. Nutr Metab (Lond). 2015;12:41. PubMed PMID: 26583036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative study of the modulation of fructose/sucrose-induced hepatic steatosis by mixed lipid formulations varying in unsaturated fatty acid content. AU - Siddiqui,Rafat A, AU - Xu,Zhidong, AU - Harvey,Kevin A, AU - Pavlina,Thomas M, AU - Becker,Michael J, AU - Zaloga,Gary P, Y1 - 2015/11/14/ PY - 2015/07/01/received PY - 2015/11/08/accepted PY - 2015/11/20/entrez PY - 2015/11/20/pubmed PY - 2015/11/20/medline KW - Fatty acids KW - Fish oil KW - Fructose KW - Hepatic steatosis KW - Lipid emulsion KW - Lipogenesis KW - Liver KW - Macadamia nut oil KW - Non-alcoholic fatty liver disease (NAFLD) KW - Nonalcoholic steatohepatitis (NASH) KW - Olive oil KW - Parenteral-nutrition-associated liver disease (PNALD) KW - Soybean oil KW - Sucrose SP - 41 EP - 41 JF - Nutrition & metabolism JO - Nutr Metab (Lond) VL - 12 N2 - BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease in developed countries. NAFLD encompasses a spectrum of diseases, ranging from hepatic steatosis to non-alcoholic steatohepatitis (NASH), cirrhosis, and liver failure. The etiology of NAFLD remains unclear but is thought to relate to increased fatty acid flux within the liver that results in toxic fatty acid metabolite production. One source of increased fatty acid flux is fructose/sucrose-induced hepatic lipogenesis. Current treatment for NAFLD encompasses dietary modifications. However, little scientific evidence exists on which to base many dietary recommendations, especially the intake of different types of carbohydrates and fats. We hypothesized that lipid mixtures of unsaturated fatty acids would inhibit lipogenesis and subsequent hepatic steatosis induced by high carbohydrate diets. The aim of this study was to examine the effects of different complex mixtures of fatty acids upon the development of fructose/sucrose-induced hepatic steatosis. METHODS: C57BL/6 mice were randomized to normocaloric chow-based diets that varied in the type of carbohydrate (starch, sucrose, fructose). Animals in each carbohydrate group were further randomized to diets that varied in lipid type (no additional lipid, soybean oil, fish oil, olive/soybean oil, macadamia nut oil). These oils were chosen based upon their content of omega-6 polyunsaturated fatty acids, omega-3 polyunsaturated fatty acids, omega-9 monounsaturated fatty acids, or omega-7 monounsaturated fatty acids. Fatty acid flux in the liver was determine by assessing hepatic lipid content (steatosis). We also assessed fatty acid levels in the plasma and liver of the animals, hepatic lipogenesis activity, hepatic stearoyl-CoA-1 desaturase activity, and hepatic elongase activity. RESULTS: Animals consumed similar amounts of the diets and maintained normal body weights throughout the study. Both sucrose and fructose induced hepatic lipogenesis and steatosis, with fructose being more potent. All mixed lipids similarly inhibited steatosis, limiting lipid content to levels found in the control (starch) animals. Lipogenesis and stearoyl-CoA-1 desaturase activity were increased in the sucrose and fructose groups. Levels of these enzymatic processes remained at baseline in all of the lipid groups. CONCLUSION: This is the first study to compare various complex lipid mixtures, based upon dietary oils with different types of long-chain fatty acids, upon development of sucrose/fructose-induced steatosis. Both carbohydrate source and lipid content appear important for the modulation of steatosis. Moderate intake of complex lipids with high unsaturated to saturated fatty acid ratios inhibited both lipogenesis and steatosis. SN - 1743-7075 UR - https://www.unboundmedicine.com/medline/citation/26583036/Comparative_study_of_the_modulation_of_fructose/sucrose_induced_hepatic_steatosis_by_mixed_lipid_formulations_varying_in_unsaturated_fatty_acid_content_ L2 - https://nutritionandmetabolism.biomedcentral.com/articles/10.1186/s12986-015-0038-x DB - PRIME DP - Unbound Medicine ER -