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Postmortem tissue distribution of acetyl fentanyl, fentanyl and their respective nor-metabolites analyzed by ultrahigh performance liquid chromatography with tandem mass spectrometry.
Forensic Sci Int. 2015 Dec; 257:435-441.FS

Abstract

In the last two years, an epidemic of fatal narcotic overdose cases has occurred in the Tampa area of Florida. Fourteen of these deaths involved fentanyl and/or the new designer drug, acetyl fentanyl. Victim demographics, case histories, toxicology findings and causes and manners of death, as well as, disposition of fentanyl derivatives and their nor-metabolites in postmortem heart blood, peripheral blood, bile, brain, liver, urine and vitreous humor are presented. In the cases involving only acetyl fentanyl (without fentanyl, n=4), the average peripheral blood acetyl fentanyl concentration was 0.467 mg/L (range 0.31 to 0.60 mg/L) and average acetyl norfentanyl concentration was 0.053 mg/L (range 0.002 to 0.086 mg/L). In the cases involving fentanyl (without acetyl fentanyl, n=7), the average peripheral blood fentanyl concentration was 0.012 mg/L (range 0.004 to 0.027 mg/L) and average norfentanyl blood concentration was 0.001 mg/L (range 0.0002 to 0.003 mg/L). In the cases involving both acetyl fentanyl and fentanyl (n=3), the average peripheral blood acetyl fentanyl concentration was 0.008 mg/L (range 0.006 to 0.012 mg/L), the average peripheral blood acetyl norfentanyl concentration was 0.001 mg/L (range 0.001 to 0.002 mg/L), the average peripheral blood fentanyl concentration was 0.018 mg/L (range 0.015 to 0.021mg/L) and the average peripheral blood norfentanyl concentration was 0.002 mg/L (range 0.001 mg/L to 0.003 mg/L). Based on the toxicology results, it is evident that when fentanyl and/or acetyl fentanyl were present, they contributed to the cause of death. A novel ultrahigh performance liquid chromatography (UPLC) tandem mass spectrometry (MS/MS) method to identify and quantify acetyl fentanyl, acetyl norfentanyl, fentanyl and norfentanyl in postmortem fluids and tissues is also presented.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA, United States. Electronic address: jlpoklis@vcu.edu.Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA, United States; Department of Pathology, Virginia Commonwealth University, Richmond, VA, United States; Department of Forensic Science, Virginia Commonwealth University, Richmond, VA, United States. Electronic address: apoklis@mcvh-vcu.edu.Department of Pathology, Virginia Commonwealth University, Richmond, VA, United States; Department of Forensic Science, Virginia Commonwealth University, Richmond, VA, United States. Electronic address: cewolf@vcu.edu.Hillsborough County Medical Examiner Department, Tampa, FL, United States. Electronic address: mainlandm@hillsboroughcounty.org.Hillsborough County Medical Examiner Department, Tampa, FL, United States. Electronic address: hairl@hillsboroughcounty.org.Hillsborough County Medical Examiner Department, Tampa, FL, United States. Electronic address: deversk@hillsboroughcounty.org.Hillsborough County Medical Examiner Department, Tampa, FL, United States. Electronic address: chrostowskil@hillsboroughcounty.org.Hillsborough County Medical Examiner Department, Tampa, FL, United States. Electronic address: arbefevillee@hillsboroughcounty.org.Hillsborough County Medical Examiner Department, Tampa, FL, United States. Electronic address: mervesm@hillsboroughcounty.org.Hillsborough County Medical Examiner Department, Tampa, FL, United States. Electronic address: pearsonjm@hillsboroughcounty.org.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

26583960

Citation

Poklis, Justin, et al. "Postmortem Tissue Distribution of Acetyl Fentanyl, Fentanyl and Their Respective Nor-metabolites Analyzed By Ultrahigh Performance Liquid Chromatography With Tandem Mass Spectrometry." Forensic Science International, vol. 257, 2015, pp. 435-441.
Poklis J, Poklis A, Wolf C, et al. Postmortem tissue distribution of acetyl fentanyl, fentanyl and their respective nor-metabolites analyzed by ultrahigh performance liquid chromatography with tandem mass spectrometry. Forensic Sci Int. 2015;257:435-441.
Poklis, J., Poklis, A., Wolf, C., Mainland, M., Hair, L., Devers, K., Chrostowski, L., Arbefeville, E., Merves, M., & Pearson, J. (2015). Postmortem tissue distribution of acetyl fentanyl, fentanyl and their respective nor-metabolites analyzed by ultrahigh performance liquid chromatography with tandem mass spectrometry. Forensic Science International, 257, 435-441. https://doi.org/10.1016/j.forsciint.2015.10.021
Poklis J, et al. Postmortem Tissue Distribution of Acetyl Fentanyl, Fentanyl and Their Respective Nor-metabolites Analyzed By Ultrahigh Performance Liquid Chromatography With Tandem Mass Spectrometry. Forensic Sci Int. 2015;257:435-441. PubMed PMID: 26583960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Postmortem tissue distribution of acetyl fentanyl, fentanyl and their respective nor-metabolites analyzed by ultrahigh performance liquid chromatography with tandem mass spectrometry. AU - Poklis,Justin, AU - Poklis,Alphonse, AU - Wolf,Carl, AU - Mainland,Mary, AU - Hair,Laura, AU - Devers,Kelly, AU - Chrostowski,Leszek, AU - Arbefeville,Elise, AU - Merves,Michele, AU - Pearson,Julia, Y1 - 2015/10/26/ PY - 2015/06/26/received PY - 2015/10/06/revised PY - 2015/10/20/accepted PY - 2015/11/20/entrez PY - 2015/11/20/pubmed PY - 2016/9/23/medline KW - Acetyl fentanyl KW - Fentanyl KW - Postmortem tissue distribution KW - UPLC/MS/MS SP - 435 EP - 441 JF - Forensic science international JO - Forensic Sci Int VL - 257 N2 - In the last two years, an epidemic of fatal narcotic overdose cases has occurred in the Tampa area of Florida. Fourteen of these deaths involved fentanyl and/or the new designer drug, acetyl fentanyl. Victim demographics, case histories, toxicology findings and causes and manners of death, as well as, disposition of fentanyl derivatives and their nor-metabolites in postmortem heart blood, peripheral blood, bile, brain, liver, urine and vitreous humor are presented. In the cases involving only acetyl fentanyl (without fentanyl, n=4), the average peripheral blood acetyl fentanyl concentration was 0.467 mg/L (range 0.31 to 0.60 mg/L) and average acetyl norfentanyl concentration was 0.053 mg/L (range 0.002 to 0.086 mg/L). In the cases involving fentanyl (without acetyl fentanyl, n=7), the average peripheral blood fentanyl concentration was 0.012 mg/L (range 0.004 to 0.027 mg/L) and average norfentanyl blood concentration was 0.001 mg/L (range 0.0002 to 0.003 mg/L). In the cases involving both acetyl fentanyl and fentanyl (n=3), the average peripheral blood acetyl fentanyl concentration was 0.008 mg/L (range 0.006 to 0.012 mg/L), the average peripheral blood acetyl norfentanyl concentration was 0.001 mg/L (range 0.001 to 0.002 mg/L), the average peripheral blood fentanyl concentration was 0.018 mg/L (range 0.015 to 0.021mg/L) and the average peripheral blood norfentanyl concentration was 0.002 mg/L (range 0.001 mg/L to 0.003 mg/L). Based on the toxicology results, it is evident that when fentanyl and/or acetyl fentanyl were present, they contributed to the cause of death. A novel ultrahigh performance liquid chromatography (UPLC) tandem mass spectrometry (MS/MS) method to identify and quantify acetyl fentanyl, acetyl norfentanyl, fentanyl and norfentanyl in postmortem fluids and tissues is also presented. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/26583960/Postmortem_tissue_distribution_of_acetyl_fentanyl_fentanyl_and_their_respective_nor_metabolites_analyzed_by_ultrahigh_performance_liquid_chromatography_with_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(15)00445-4 DB - PRIME DP - Unbound Medicine ER -