Tags

Type your tag names separated by a space and hit enter

Nopaline-type Ti plasmid of Agrobacterium encodes a VirF-like functional F-box protein.
Sci Rep. 2015 Nov 20; 5:16610.SR

Abstract

During Agrobacterium-mediated genetic transformation of plants, several bacterial virulence (Vir) proteins are translocated into the host cell to facilitate infection. One of the most important of such translocated factors is VirF, an F-box protein produced by octopine strains of Agrobacterium, which presumably facilitates proteasomal uncoating of the invading T-DNA from its associated proteins. The presence of VirF also is thought to be involved in differences in host specificity between octopine and nopaline strains of Agrobacterium, with the current dogma being that no functional VirF is encoded by nopaline strains. Here, we show that a protein with homology to octopine VirF is encoded by the Ti plasmid of the nopaline C58 strain of Agrobacterium. This protein, C58VirF, possesses the hallmarks of functional F-box proteins: it contains an active F-box domain and specifically interacts, via its F-box domain, with SKP1-like (ASK) protein components of the plant ubiquitin/proteasome system. Thus, our data suggest that nopaline strains of Agrobacterium have evolved to encode a functional F-box protein VirF.

Authors+Show Affiliations

Stony Brook University, Department of Biochemistry and Cell Biology, Stony Brook, NY 11794-5215, USA.Stony Brook University, Department of Biochemistry and Cell Biology, Stony Brook, NY 11794-5215, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26586289

Citation

Lacroix, Benoît, and Vitaly Citovsky. "Nopaline-type Ti Plasmid of Agrobacterium Encodes a VirF-like Functional F-box Protein." Scientific Reports, vol. 5, 2015, p. 16610.
Lacroix B, Citovsky V. Nopaline-type Ti plasmid of Agrobacterium encodes a VirF-like functional F-box protein. Sci Rep. 2015;5:16610.
Lacroix, B., & Citovsky, V. (2015). Nopaline-type Ti plasmid of Agrobacterium encodes a VirF-like functional F-box protein. Scientific Reports, 5, 16610. https://doi.org/10.1038/srep16610
Lacroix B, Citovsky V. Nopaline-type Ti Plasmid of Agrobacterium Encodes a VirF-like Functional F-box Protein. Sci Rep. 2015 Nov 20;5:16610. PubMed PMID: 26586289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nopaline-type Ti plasmid of Agrobacterium encodes a VirF-like functional F-box protein. AU - Lacroix,Benoît, AU - Citovsky,Vitaly, Y1 - 2015/11/20/ PY - 2015/06/19/received PY - 2015/10/16/accepted PY - 2015/11/21/entrez PY - 2015/11/21/pubmed PY - 2016/10/12/medline SP - 16610 EP - 16610 JF - Scientific reports JO - Sci Rep VL - 5 N2 - During Agrobacterium-mediated genetic transformation of plants, several bacterial virulence (Vir) proteins are translocated into the host cell to facilitate infection. One of the most important of such translocated factors is VirF, an F-box protein produced by octopine strains of Agrobacterium, which presumably facilitates proteasomal uncoating of the invading T-DNA from its associated proteins. The presence of VirF also is thought to be involved in differences in host specificity between octopine and nopaline strains of Agrobacterium, with the current dogma being that no functional VirF is encoded by nopaline strains. Here, we show that a protein with homology to octopine VirF is encoded by the Ti plasmid of the nopaline C58 strain of Agrobacterium. This protein, C58VirF, possesses the hallmarks of functional F-box proteins: it contains an active F-box domain and specifically interacts, via its F-box domain, with SKP1-like (ASK) protein components of the plant ubiquitin/proteasome system. Thus, our data suggest that nopaline strains of Agrobacterium have evolved to encode a functional F-box protein VirF. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/26586289/Nopaline_type_Ti_plasmid_of_Agrobacterium_encodes_a_VirF_like_functional_F_box_protein_ L2 - http://dx.doi.org/10.1038/srep16610 DB - PRIME DP - Unbound Medicine ER -