Aquaporin gene expression and physiological responses of Robinia pseudoacacia L. to the mycorrhizal fungus Rhizophagus irregularis and drought stress.
Mycorrhiza. 2016 May; 26(4):311-23.M

Abstract

The influence of arbuscular mycorrhiza (AM) and drought stress on aquaporin (AQP) gene expression, water status, and photosynthesis was investigated in black locust (Robinia pseudoacacia L.). Seedlings were grown in potted soil inoculated without or with the AM fungus Rhizophagus irregularis, under well-watered and drought stress conditions. Six full-length AQP complementary DNAs (cDNAs) were isolated from Robinia pseudoacacia, named RpTIP1;1, RpTIP1;3, RpTIP2;1, RpPIP1;1, RpPIP1;3, and RpPIP2;1. A phylogenetic analysis of deduced amino acid sequences demonstrated that putative proteins coded by these RpAQP genes belong to the water channel protein family. Expression analysis revealed higher RpPIP expression in roots while RpTIP expression was higher in leaves, except for RpTIP1;3. AM symbiosis regulated host plant AQPs, and the expression of RpAQP genes in mycorrhizal plants depended on soil water condition and plant tissue. Positive effects were observed for plant physiological parameters in AM plants, which had higher dry mass and lower water saturation deficit and electrolyte leakage than non-AM plants. Rhizophagus irregularis inoculation also slightly increased leaf net photosynthetic rate and stomatal conductance under well-watered and drought stress conditions. These findings suggest that AM symbiosis can enhance the drought tolerance in Robinia pseudoacacia plants by regulating the expression of RpAQP genes, and by improving plant biomass, tissue water status, and leaf photosynthesis in host seedlings.

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Publisher Full Text (DOI)

Authors+Show Affiliations

He F

College of Forestry, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Zhang H

College of Forestry, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Tang M

College of Forestry, Northwest A&F University, Yangling, 712100, Shaanxi, China. tangm@nwsuaf.edu.cn.

MeSH

AquaporinsDroughtsGlomeromycotaMycorrhizaePhylogenyPlant ProteinsPlant RootsRobiniaSeedlingsStress, PhysiologicalSymbiosis

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26590998

Citation

He, Fei, et al. "Aquaporin Gene Expression and Physiological Responses of Robinia Pseudoacacia L. to the Mycorrhizal Fungus Rhizophagus Irregularis and Drought Stress." Mycorrhiza, vol. 26, no. 4, 2016, pp. 311-23.

He F, Zhang H, Tang M. Aquaporin gene expression and physiological responses of Robinia pseudoacacia L. to the mycorrhizal fungus Rhizophagus irregularis and drought stress. Mycorrhiza. 2016;26(4):311-23.

He, F., Zhang, H., & Tang, M. (2016). Aquaporin gene expression and physiological responses of Robinia pseudoacacia L. to the mycorrhizal fungus Rhizophagus irregularis and drought stress. Mycorrhiza, 26(4), 311-23. https://doi.org/10.1007/s00572-015-0670-3

He F, Zhang H, Tang M. Aquaporin Gene Expression and Physiological Responses of Robinia Pseudoacacia L. to the Mycorrhizal Fungus Rhizophagus Irregularis and Drought Stress. Mycorrhiza. 2016;26(4):311-23. PubMed PMID: 26590998.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Aquaporin gene expression and physiological responses of Robinia pseudoacacia L. to the mycorrhizal fungus Rhizophagus irregularis and drought stress. AU - He,Fei, AU - Zhang,Haoqiang, AU - Tang,Ming, Y1 - 2015/11/21/ PY - 2015/09/10/received PY - 2015/11/13/accepted PY - 2015/11/23/entrez PY - 2015/11/23/pubmed PY - 2016/12/15/medline KW - Aquaporins KW - Arbuscular mycorrhiza KW - Drought stress KW - Rhizophagus irregularis KW - Robinia pseudoacacia SP - 311 EP - 23 JF - Mycorrhiza JO - Mycorrhiza VL - 26 IS - 4 N2 - The influence of arbuscular mycorrhiza (AM) and drought stress on aquaporin (AQP) gene expression, water status, and photosynthesis was investigated in black locust (Robinia pseudoacacia L.). Seedlings were grown in potted soil inoculated without or with the AM fungus Rhizophagus irregularis, under well-watered and drought stress conditions. Six full-length AQP complementary DNAs (cDNAs) were isolated from Robinia pseudoacacia, named RpTIP1;1, RpTIP1;3, RpTIP2;1, RpPIP1;1, RpPIP1;3, and RpPIP2;1. A phylogenetic analysis of deduced amino acid sequences demonstrated that putative proteins coded by these RpAQP genes belong to the water channel protein family. Expression analysis revealed higher RpPIP expression in roots while RpTIP expression was higher in leaves, except for RpTIP1;3. AM symbiosis regulated host plant AQPs, and the expression of RpAQP genes in mycorrhizal plants depended on soil water condition and plant tissue. Positive effects were observed for plant physiological parameters in AM plants, which had higher dry mass and lower water saturation deficit and electrolyte leakage than non-AM plants. Rhizophagus irregularis inoculation also slightly increased leaf net photosynthetic rate and stomatal conductance under well-watered and drought stress conditions. These findings suggest that AM symbiosis can enhance the drought tolerance in Robinia pseudoacacia plants by regulating the expression of RpAQP genes, and by improving plant biomass, tissue water status, and leaf photosynthesis in host seedlings. SN - 1432-1890 UR - https://www.unboundmedicine.com/medline/citation/26590998/Aquaporin_gene_expression_and_physiological_responses_of_Robinia_pseudoacacia_L__to_the_mycorrhizal_fungus_Rhizophagus_irregularis_and_drought_stress_ DB - PRIME DP - Unbound Medicine ER -

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Aquaporin gene expression and physiological responses of Robinia pseudoacacia L. to the mycorrhizal fungus Rhizophagus irregularis and drought stress.Mycorrhiza. 2016 May; 26(4):311-23.M