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Oleanolic acid activates daf-16 to increase lifespan in Caenorhabditis elegans.
Biochem Biophys Res Commun 2015; 468(4):843-9BB

Abstract

Oleanolic acid (OA) is an active ingredient in natural plants. It has been reported to possess a variety of pharmacological activities, but very little is known about its effects of anti-aging. We investigate here whether OA has an impact on longevity in vivo, and more specifically, we have examined effects of OA on the lifespan and stress tolerance in Caenorhabditis elegans (C. elegans). Our results showed that OA could extend the lifespan, increase its stress resistance and reduce the intracellular reactive oxygen species (ROS) in wild-type worms. Moreover, we have found that OA-induced longevity may not be associated with the calorie restriction (CR) mechanism. Our mechanistic studies using daf-16 loss-of-function mutant strains (GR1307) indicated that the extension of lifespan by OA requires daf-16. In addition, OA treatment could also modulate the nuclear localization, and the quantitative real-time PCR results revealed that up-regulation of daf-16 target genes such as sod-3, hsp-16.2 and ctl-1 could prolong lifespan and increase stress response in C. elegans. This study overall uncovers the longevity effect of OA and its underpinning mechanisms.

Authors+Show Affiliations

Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China. Electronic address: lijunzhou@tju.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26592451

Citation

Zhang, Jiaolong, et al. "Oleanolic Acid Activates Daf-16 to Increase Lifespan in Caenorhabditis Elegans." Biochemical and Biophysical Research Communications, vol. 468, no. 4, 2015, pp. 843-9.
Zhang J, Lu L, Zhou L. Oleanolic acid activates daf-16 to increase lifespan in Caenorhabditis elegans. Biochem Biophys Res Commun. 2015;468(4):843-9.
Zhang, J., Lu, L., & Zhou, L. (2015). Oleanolic acid activates daf-16 to increase lifespan in Caenorhabditis elegans. Biochemical and Biophysical Research Communications, 468(4), pp. 843-9. doi:10.1016/j.bbrc.2015.11.042.
Zhang J, Lu L, Zhou L. Oleanolic Acid Activates Daf-16 to Increase Lifespan in Caenorhabditis Elegans. Biochem Biophys Res Commun. 2015 Dec 25;468(4):843-9. PubMed PMID: 26592451.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oleanolic acid activates daf-16 to increase lifespan in Caenorhabditis elegans. AU - Zhang,Jiaolong, AU - Lu,Lulu, AU - Zhou,Lijun, Y1 - 2015/11/25/ PY - 2015/11/04/received PY - 2015/11/09/accepted PY - 2015/11/24/entrez PY - 2015/11/26/pubmed PY - 2016/4/20/medline KW - Caenorhabditis elegans KW - Lifespan KW - Oleanolic acid KW - daf-16 SP - 843 EP - 9 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 468 IS - 4 N2 - Oleanolic acid (OA) is an active ingredient in natural plants. It has been reported to possess a variety of pharmacological activities, but very little is known about its effects of anti-aging. We investigate here whether OA has an impact on longevity in vivo, and more specifically, we have examined effects of OA on the lifespan and stress tolerance in Caenorhabditis elegans (C. elegans). Our results showed that OA could extend the lifespan, increase its stress resistance and reduce the intracellular reactive oxygen species (ROS) in wild-type worms. Moreover, we have found that OA-induced longevity may not be associated with the calorie restriction (CR) mechanism. Our mechanistic studies using daf-16 loss-of-function mutant strains (GR1307) indicated that the extension of lifespan by OA requires daf-16. In addition, OA treatment could also modulate the nuclear localization, and the quantitative real-time PCR results revealed that up-regulation of daf-16 target genes such as sod-3, hsp-16.2 and ctl-1 could prolong lifespan and increase stress response in C. elegans. This study overall uncovers the longevity effect of OA and its underpinning mechanisms. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/26592451/Oleanolic_acid_activates_daf_16_to_increase_lifespan_in_Caenorhabditis_elegans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(15)30912-8 DB - PRIME DP - Unbound Medicine ER -