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Switch-on fluorescence scheme for antibiotics based on a magnetic composite probe with aptamer and hemin/G-quadruplex coimmobilized nano-Pt-luminol as signal tracer.
Talanta. 2016 Jan 15; 147:296-301.T

Abstract

A selective and facile fluorescence "switch-on" scheme is developed to detect antibiotics residues in food, using chloramphenicol (CAP) as model, based on a novel magnetic aptamer probe (aptamer-Pt-luminol nanocomposite labeled with hemin/G-quadruplex). Firstly, the composite probe is prepared through the immuno-reactions between the capture beads (anti-dsDNA antibody labeled on magnetic Dynabeads) and the nanotracer (nano-Pt-luminol labeled with double-strand aptamer, as ds-Apt, and hemin/G-quadruplex). When the composite probe is mixed with CAP, the aptamer preferentially reacted with CAP to decompose the double-strand aptamer to ssDNA, which cannot be recognized by the anti-dsDNA antibody on the capture probes. Thus, after magnetic separation, the nanotracer can be released into the supernatant. Because the hemin/G-quadruplex and PtNPs in nanotracer can catalyze luminol-H2O2 system to emit fluorescence. Thus a dual-amplified "switch-on" signal appeared, of which intensity is proportional to the concentration of CAP between 0.001 and 100ng mL(-1) with detection limit of 0.0005ng mL(-1) (S/N=3). Besides, our method has good selectivity and was employed for CAP detection in real milk samples. The results agree well with those from conventional gas chromatograph-mass spectrometer (GC-MS). The switch-on signal is produced by one-step substitution reaction between aptamer in nanotracer and target. When the analyte is changed, the probe can be refabricated only by changing the corresponding aptamer. Thus, all features above prove our strategy to be a facile, feasible and selective method in antibiotics screening for food safety.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China.

Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China.

Key Laboratory of Asymmetric Synthesis and Chirotechnology of Sichuan Province, Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 10049, China.

Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China.

Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China.

Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China.

Faculty of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210000, China.

MeSH

Anti-Bacterial AgentsAptamers, NucleotideBase SequenceBiosensing TechniquesChloramphenicolFluorescent DyesG-QuadruplexesHeminHydrogen PeroxideLimit of DetectionLuminolMagnetsModels, MolecularNanostructuresPlatinumSpectrometry, Fluorescence

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26592610

Citation

Miao, Yang-Bao, et al. "Switch-on Fluorescence Scheme for Antibiotics Based On a Magnetic Composite Probe With Aptamer and hemin/G-quadruplex Coimmobilized nano-Pt-luminol as Signal Tracer." Talanta, vol. 147, 2016, pp. 296-301.

Miao YB, Gan N, Ren HX, et al. Switch-on fluorescence scheme for antibiotics based on a magnetic composite probe with aptamer and hemin/G-quadruplex coimmobilized nano-Pt-luminol as signal tracer. Talanta. 2016;147:296-301.

Miao, Y. B., Gan, N., Ren, H. X., Li, T., Cao, Y., Hu, F., & Chen, Y. (2016). Switch-on fluorescence scheme for antibiotics based on a magnetic composite probe with aptamer and hemin/G-quadruplex coimmobilized nano-Pt-luminol as signal tracer. Talanta, 147, 296-301. https://doi.org/10.1016/j.talanta.2015.10.005

Miao YB, et al. Switch-on Fluorescence Scheme for Antibiotics Based On a Magnetic Composite Probe With Aptamer and hemin/G-quadruplex Coimmobilized nano-Pt-luminol as Signal Tracer. Talanta. 2016 Jan 15;147:296-301. PubMed PMID: 26592610.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Switch-on fluorescence scheme for antibiotics based on a magnetic composite probe with aptamer and hemin/G-quadruplex coimmobilized nano-Pt-luminol as signal tracer. AU - Miao,Yang-Bao, AU - Gan,Ning, AU - Ren,Hong-Xia, AU - Li,Tianhua, AU - Cao,Yuting, AU - Hu,Futao, AU - Chen,Yinji, Y1 - 2015/10/03/ PY - 2015/07/28/received PY - 2015/09/28/revised PY - 2015/10/01/accepted PY - 2015/11/24/entrez PY - 2015/11/26/pubmed PY - 2016/9/1/medline KW - Antibiotics KW - Chloramphenicol KW - Fluorescence aptamer probes KW - Hemin/G-quadruplex nanotracer KW - Pt–luminol capture bead KW - Switch on SP - 296 EP - 301 JF - Talanta JO - Talanta VL - 147 N2 - A selective and facile fluorescence "switch-on" scheme is developed to detect antibiotics residues in food, using chloramphenicol (CAP) as model, based on a novel magnetic aptamer probe (aptamer-Pt-luminol nanocomposite labeled with hemin/G-quadruplex). Firstly, the composite probe is prepared through the immuno-reactions between the capture beads (anti-dsDNA antibody labeled on magnetic Dynabeads) and the nanotracer (nano-Pt-luminol labeled with double-strand aptamer, as ds-Apt, and hemin/G-quadruplex). When the composite probe is mixed with CAP, the aptamer preferentially reacted with CAP to decompose the double-strand aptamer to ssDNA, which cannot be recognized by the anti-dsDNA antibody on the capture probes. Thus, after magnetic separation, the nanotracer can be released into the supernatant. Because the hemin/G-quadruplex and PtNPs in nanotracer can catalyze luminol-H2O2 system to emit fluorescence. Thus a dual-amplified "switch-on" signal appeared, of which intensity is proportional to the concentration of CAP between 0.001 and 100ng mL(-1) with detection limit of 0.0005ng mL(-1) (S/N=3). Besides, our method has good selectivity and was employed for CAP detection in real milk samples. The results agree well with those from conventional gas chromatograph-mass spectrometer (GC-MS). The switch-on signal is produced by one-step substitution reaction between aptamer in nanotracer and target. When the analyte is changed, the probe can be refabricated only by changing the corresponding aptamer. Thus, all features above prove our strategy to be a facile, feasible and selective method in antibiotics screening for food safety. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/26592610/Switch_on_fluorescence_scheme_for_antibiotics_based_on_a_magnetic_composite_probe_with_aptamer_and_hemin/G_quadruplex_coimmobilized_nano_Pt_luminol_as_signal_tracer_ DB - PRIME DP - Unbound Medicine ER -