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Anti-apoptotic effect of modified Chunsimyeolda-tang, a traditional Korean herbal formula, on MPTP-induced neuronal cell death in a Parkinson's disease mouse model.
J Ethnopharmacol. 2015 Dec 24; 176:336-44.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The modified-Chungsimyeolda-tang (DG) is an important traditional Korean herbal formula used in traditional oriental medicine for treatment of cerebrovascular disorders, including stroke. The formula is based on the book "Dongui Sasang Shinpyun".

AIM OF THE STUDY

In the previous studies, the neuroprotective effect of DG is demonstrated in an in vitro Parkinson's disease (PD) model, and in this study, the 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) animal model of PD is used to evaluate the behavioral effect of DG and possible mechanism through anti-apoptosis of DG. 6-Hydroxydopamine (6-OHDA) also is used to evaluate the anti-apoptosis effect of DG in SH-SY5Y cells.

MATERIALS AND METHODS

MPTP was used to evaluate the behavioral damage and neurotoxicity in mice. The bradykinesia symptom was measured by a Pole test and a Rota-rod test in mice. Also the loss of tyrosine hydroxylase (TH)-positive neurons induced by MPTP was examined by an immunohistochemical assay. The DG-mediated anti-apoptosis effect was measured using an immunoblotting assay with apoptosis-related markers such as Bax and cleaved caspase-3. DG and 1-methyl-4-phenylpyridinium (MPP(+)) were co-treated with primary dopaminergic neurons to evaluate the protective effect of DG. The expression of caspase-3 and PARP was measured to detect the protective effect of DG from the damage by 6-OHDA.

RESULTS AND CONCLUSIONS

The treatment with DG resulted in prophylactic effects on MPTP-induced Parkinsonian bradykinesia and the immunohistochemical analysis showed that DG provided the neuroprotection against the MPP(+)-induced dopaminergic neurons loss through the anti-apoptosis effect. The present results suggested that it might be possible to use DG for the prevention of substantia nigra pars compacta (SNpc) degeneration induced by exposure to the toxic substances, such as MPTP/MPP(+), in PD mouse model.

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Authors+Show Affiliations

Li H
Natural Products Research Center, Korea Institute of Science and Technology, Gangneung 210-340, Republic of Korea; Department of Biological Chemistry, University of Science & Technology (UST), Daejeon 305-350, Republic of Korea; College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China.
Park G
Department of Life and Nanopharmaceutical Science and Kyung Hee East-West Pharmaceutical Research Institute, Kyung Hee University, Seoul 130-701, Republic of Korea.
Bae N
Natural Products Research Center, Korea Institute of Science and Technology, Gangneung 210-340, Republic of Korea; Department of Sasang Constitution Medicine, Pusan National University School of Korean Medicine, Yangsan 626-870, Republic of Korea.
Kim J
Natural Products Research Center, Korea Institute of Science and Technology, Gangneung 210-340, Republic of Korea.
Oh MS
Department of Life and Nanopharmaceutical Science and Kyung Hee East-West Pharmaceutical Research Institute, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: msohok@khu.ac.kr.
Yang HO
Natural Products Research Center, Korea Institute of Science and Technology, Gangneung 210-340, Republic of Korea; Department of Biological Chemistry, University of Science & Technology (UST), Daejeon 305-350, Republic of Korea. Electronic address: hoyang@kist.re.kr.

MeSH

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridineAnimalsAntiparkinson AgentsApoptosisBehavior, AnimalBrainCaspase 3Cell DeathCell Line, TumorCells, CulturedDisease Models, AnimalDopaminergic NeuronsHumansKoreaMaleMedicine, TraditionalMice, Inbred C57BLNeuroprotective AgentsNeurotoxinsParkinson DiseasePhytotherapyPlant ExtractsRats, Sprague-Dawleybcl-2-Associated X Protein

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26593210

Citation

Li, Huan, et al. "Anti-apoptotic Effect of Modified Chunsimyeolda-tang, a Traditional Korean Herbal Formula, On MPTP-induced Neuronal Cell Death in a Parkinson's Disease Mouse Model." Journal of Ethnopharmacology, vol. 176, 2015, pp. 336-44.
Li H, Park G, Bae N, et al. Anti-apoptotic effect of modified Chunsimyeolda-tang, a traditional Korean herbal formula, on MPTP-induced neuronal cell death in a Parkinson's disease mouse model. J Ethnopharmacol. 2015;176:336-44.
Li, H., Park, G., Bae, N., Kim, J., Oh, M. S., & Yang, H. O. (2015). Anti-apoptotic effect of modified Chunsimyeolda-tang, a traditional Korean herbal formula, on MPTP-induced neuronal cell death in a Parkinson's disease mouse model. Journal of Ethnopharmacology, 176, 336-44. https://doi.org/10.1016/j.jep.2015.11.013
Li H, et al. Anti-apoptotic Effect of Modified Chunsimyeolda-tang, a Traditional Korean Herbal Formula, On MPTP-induced Neuronal Cell Death in a Parkinson's Disease Mouse Model. J Ethnopharmacol. 2015 Dec 24;176:336-44. PubMed PMID: 26593210.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-apoptotic effect of modified Chunsimyeolda-tang, a traditional Korean herbal formula, on MPTP-induced neuronal cell death in a Parkinson's disease mouse model. AU - Li,Huan, AU - Park,Gunhyuk, AU - Bae,Nayoung, AU - Kim,Joonki, AU - Oh,Myung Sook, AU - Yang,Hyun Ok, Y1 - 2015/11/23/ PY - 2015/06/22/received PY - 2015/11/01/revised PY - 2015/11/04/accepted PY - 2015/11/24/entrez PY - 2015/11/26/pubmed PY - 2016/10/7/medline KW - 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine KW - 6-Hydroxydopamine KW - Apoptosis KW - Modified-Chungsimyeolda-tang KW - Neuroprotection KW - Parkinson's disease SP - 336 EP - 44 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 176 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The modified-Chungsimyeolda-tang (DG) is an important traditional Korean herbal formula used in traditional oriental medicine for treatment of cerebrovascular disorders, including stroke. The formula is based on the book "Dongui Sasang Shinpyun". AIM OF THE STUDY: In the previous studies, the neuroprotective effect of DG is demonstrated in an in vitro Parkinson's disease (PD) model, and in this study, the 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) animal model of PD is used to evaluate the behavioral effect of DG and possible mechanism through anti-apoptosis of DG. 6-Hydroxydopamine (6-OHDA) also is used to evaluate the anti-apoptosis effect of DG in SH-SY5Y cells. MATERIALS AND METHODS: MPTP was used to evaluate the behavioral damage and neurotoxicity in mice. The bradykinesia symptom was measured by a Pole test and a Rota-rod test in mice. Also the loss of tyrosine hydroxylase (TH)-positive neurons induced by MPTP was examined by an immunohistochemical assay. The DG-mediated anti-apoptosis effect was measured using an immunoblotting assay with apoptosis-related markers such as Bax and cleaved caspase-3. DG and 1-methyl-4-phenylpyridinium (MPP(+)) were co-treated with primary dopaminergic neurons to evaluate the protective effect of DG. The expression of caspase-3 and PARP was measured to detect the protective effect of DG from the damage by 6-OHDA. RESULTS AND CONCLUSIONS: The treatment with DG resulted in prophylactic effects on MPTP-induced Parkinsonian bradykinesia and the immunohistochemical analysis showed that DG provided the neuroprotection against the MPP(+)-induced dopaminergic neurons loss through the anti-apoptosis effect. The present results suggested that it might be possible to use DG for the prevention of substantia nigra pars compacta (SNpc) degeneration induced by exposure to the toxic substances, such as MPTP/MPP(+), in PD mouse model. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/26593210/Anti_apoptotic_effect_of_modified_Chunsimyeolda_tang_a_traditional_Korean_herbal_formula_on_MPTP_induced_neuronal_cell_death_in_a_Parkinson's_disease_mouse_model_ DB - PRIME DP - Unbound Medicine ER -