TY - JOUR T1 - Structural characterization of phenolic compounds and antioxidant activity of the phenolic-rich fraction from defatted adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) seed meal. AU - Wang,Lifeng, AU - Chen,Chao, AU - Su,Anxiang, AU - Zhang,Yiyi, AU - Yuan,Jian, AU - Ju,Xingrong, Y1 - 2015/09/25/ PY - 2015/04/20/received PY - 2015/08/20/revised PY - 2015/09/23/accepted PY - 2015/11/24/entrez PY - 2015/11/26/pubmed PY - 2016/8/23/medline KW - Antioxidant activity KW - Defatted adlay seed meal KW - Ferulic acid KW - Fractionation KW - Phenolic compounds KW - Quercetin KW - Rutin SP - 509 EP - 17 JF - Food chemistry JO - Food Chem VL - 196 N2 - The current study aims to investigate the antioxidant activities of various extracts from defatted adlay seed meal (DASM) based on the oxygen radical absorbance capacity (ORAC) assay, peroxyl radical scavenging capacity (PSC) assay and cellular antioxidant activity (CAA) assay. Of all the fractions, the n-butanol fraction exhibited the highest antioxidant activity, followed by crude acetone extract and aqueous fractions. Of the three sub-fractions obtained by Sephadex LH-20 chromatography, sub-fraction 3 possessed the highest antioxidant activity and total phenolic content. There was a strong positive correlation between the total phenolic content and the antioxidant activity. Based on HPLC-DAD-ESI-MS/MS analysis, the most abundant phenolic acid in sub-fraction 3 of DASM was ferulic acid at 67.28 mg/g, whereas the predominant flavonoid was rutin at 41.11 mg/g. Of the major individual compounds in sub-fraction 3, p-coumaric acid exhibited the highest ORAC values, and quercetin exhibited the highest PSC values and CAA values. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/26593521/Structural_characterization_of_phenolic_compounds_and_antioxidant_activity_of_the_phenolic_rich_fraction_from_defatted_adlay__Coix_lachryma_jobi_L__var__ma_yuen_Stapf__seed_meal_ DB - PRIME DP - Unbound Medicine ER -