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Hepcidin determination in dried blood by microfluidic LC-MS/MS: comparison of DBS and volumetric absorptive microsampling for matrix effect and recovery.
Bioanalysis. 2015 Nov; 7(21):2789-99.B

Abstract

BACKGROUND

Dried blood analysis experiences a growing interest due to practical, ethical and financial advantages compared with classical wet plasma or serum analysis. Besides classical DBS, new alternatives are commercialized as volumetric absorptive microsampling (VAMS) that are expected to overcome hematocrit influence.

RESULTS

The feasibility of hepcidin (a peptide hormone) extraction and determination from DBS and VAMS blood sampling was investigated. Experimental design was used to determine the optimal extraction conditions. Matrix effect and extraction recovery were studied and a special attention was paid to phospholipid removal.

CONCLUSION

The data suggest that the combination of VAMS and phospholipid removal plates provides low matrix effect and high sensitivity, and constitutes an easy and promising protocol for hepcidin analysis.

Authors+Show Affiliations

Laboratory for the Analysis of Medicines, Department of Pharmacy, CIRM, University of Liege, Belgium.Department of Clinical Chemistry, CHU, University of Liege, Belgium.Laboratory for the Analysis of Medicines, Department of Pharmacy, CIRM, University of Liege, Belgium.Laboratory for the Analysis of Medicines, Department of Pharmacy, CIRM, University of Liege, Belgium.Department of Clinical Chemistry, CHU, University of Liege, Belgium.Laboratory for the Analysis of Medicines, Department of Pharmacy, CIRM, University of Liege, Belgium.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26595680

Citation

Houbart, Virginie, et al. "Hepcidin Determination in Dried Blood By Microfluidic LC-MS/MS: Comparison of DBS and Volumetric Absorptive Microsampling for Matrix Effect and Recovery." Bioanalysis, vol. 7, no. 21, 2015, pp. 2789-99.
Houbart V, Cobraiville G, Servais AC, et al. Hepcidin determination in dried blood by microfluidic LC-MS/MS: comparison of DBS and volumetric absorptive microsampling for matrix effect and recovery. Bioanalysis. 2015;7(21):2789-99.
Houbart, V., Cobraiville, G., Servais, A. C., Napp, A., Merville, M. P., & Fillet, M. (2015). Hepcidin determination in dried blood by microfluidic LC-MS/MS: comparison of DBS and volumetric absorptive microsampling for matrix effect and recovery. Bioanalysis, 7(21), 2789-99. https://doi.org/10.4155/bio.15.181
Houbart V, et al. Hepcidin Determination in Dried Blood By Microfluidic LC-MS/MS: Comparison of DBS and Volumetric Absorptive Microsampling for Matrix Effect and Recovery. Bioanalysis. 2015;7(21):2789-99. PubMed PMID: 26595680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepcidin determination in dried blood by microfluidic LC-MS/MS: comparison of DBS and volumetric absorptive microsampling for matrix effect and recovery. AU - Houbart,Virginie, AU - Cobraiville,Gaël, AU - Servais,Anne-Catherine, AU - Napp,Aurore, AU - Merville,Marie-Paule, AU - Fillet,Marianne, Y1 - 2015/11/13/ PY - 2015/11/24/entrez PY - 2015/11/26/pubmed PY - 2016/9/14/medline SP - 2789 EP - 99 JF - Bioanalysis JO - Bioanalysis VL - 7 IS - 21 N2 - BACKGROUND: Dried blood analysis experiences a growing interest due to practical, ethical and financial advantages compared with classical wet plasma or serum analysis. Besides classical DBS, new alternatives are commercialized as volumetric absorptive microsampling (VAMS) that are expected to overcome hematocrit influence. RESULTS: The feasibility of hepcidin (a peptide hormone) extraction and determination from DBS and VAMS blood sampling was investigated. Experimental design was used to determine the optimal extraction conditions. Matrix effect and extraction recovery were studied and a special attention was paid to phospholipid removal. CONCLUSION: The data suggest that the combination of VAMS and phospholipid removal plates provides low matrix effect and high sensitivity, and constitutes an easy and promising protocol for hepcidin analysis. SN - 1757-6199 UR - https://www.unboundmedicine.com/medline/citation/26595680/Hepcidin_determination_in_dried_blood_by_microfluidic_LC_MS/MS:_comparison_of_DBS_and_volumetric_absorptive_microsampling_for_matrix_effect_and_recovery_ L2 - https://www.future-science.com/doi/full/10.4155/bio.15.181?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -