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Efficacy of medicinal essential oils against pathogenic Malassezia sp. isolates.
J Mycol Med. 2016 Mar; 26(1):28-34.JM

Abstract

OBJECTIVES

The purposes of this study were to evaluate the distribution pattern and population size of Malassezia species in dogs with atopic dermatitis (AD) and the inhibitory efficacy of Zataria multiflora, Thymus kotschyanus, Mentha spicata, Artemisia sieberi, Rosmarinus officinalis and Heracleum persicum essential oils against pathogenic Malassezia isolates.

METHODS

The samples were collected from 5 different anatomical sites of 33 atopic dogs and cultured onto modified Dixon agar (MDA) and Sabouraud dextrose agar (SDA) media. The essential oil extraction was performed by steam distillation using Clevenger system. Anti-Malassezia efficacy of medicinal essential oils and standard drugs was evaluated using broth microdilution method.

RESULTS

A total of 103 yeast colonies were isolated from dogs with AD. Eight different Malassezia species were identified as follows: Malassezia pachydermatis (81.4%), M. globosa (7.8%), M. restricta (3.9%), M. sloofiae (2.9%), M. furfur (1%), M. nana (1%), M. obtusa (1%) and M. sympodialis (1%). The most and least infected sites were: anal (21.2%) and ear (10.6%) respectively. M. pachydermatis was the most frequent Malassezia species isolated from both skin and mucosa of dogs with AD. Antifungal susceptibility test revealed the inhibitory efficacy of essential oils on pathogenic Malassezia isolates with minimum inhibitory concentration (MIC(90)) values ranging from 30 to 850 μg/mL. Among the tested oils, Z. multiflora and T. kotschyanus exhibited the highest inhibitory effects (P<0.05).

CONCLUSION

The essential oils of Z. multiflora and T. kotschyanus showed strong antifungal activity against pathogenic Malassezia species tested.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Khosravi AR
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Electronic address: khosravi@ut.ac.ir.
Shokri H
Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Fahimirad S
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

MeSH

AnimalsAntifungal AgentsArtemisiaDermatitis, AtopicDog DiseasesDogsDrug Resistance, FungalFemaleHeracleumLamiaceaeMalasseziaMaleMentha spicataMicrobial Sensitivity TestsOils, VolatilePlant ExtractsRosmarinusThymus Plant

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26597143

Citation

Khosravi, A R., et al. "Efficacy of Medicinal Essential Oils Against Pathogenic Malassezia Sp. Isolates." Journal De Mycologie Medicale, vol. 26, no. 1, 2016, pp. 28-34.
Khosravi AR, Shokri H, Fahimirad S. Efficacy of medicinal essential oils against pathogenic Malassezia sp. isolates. J Mycol Med. 2016;26(1):28-34.
Khosravi, A. R., Shokri, H., & Fahimirad, S. (2016). Efficacy of medicinal essential oils against pathogenic Malassezia sp. isolates. Journal De Mycologie Medicale, 26(1), 28-34. https://doi.org/10.1016/j.mycmed.2015.10.012
Khosravi AR, Shokri H, Fahimirad S. Efficacy of Medicinal Essential Oils Against Pathogenic Malassezia Sp. Isolates. J Mycol Med. 2016;26(1):28-34. PubMed PMID: 26597143.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficacy of medicinal essential oils against pathogenic Malassezia sp. isolates. AU - Khosravi,A R, AU - Shokri,H, AU - Fahimirad,S, Y1 - 2015/11/17/ PY - 2015/08/18/received PY - 2015/10/19/revised PY - 2015/10/21/accepted PY - 2015/11/25/entrez PY - 2015/11/26/pubmed PY - 2017/4/20/medline KW - Atopic dermatitis KW - Atopique KW - Chien KW - Dermatite atopique KW - Dog KW - Essential oils KW - Huiles essentielles KW - Malassezia KW - Malassezia pachydermatis KW - Malassezia sp. SP - 28 EP - 34 JF - Journal de mycologie medicale JO - J Mycol Med VL - 26 IS - 1 N2 - OBJECTIVES: The purposes of this study were to evaluate the distribution pattern and population size of Malassezia species in dogs with atopic dermatitis (AD) and the inhibitory efficacy of Zataria multiflora, Thymus kotschyanus, Mentha spicata, Artemisia sieberi, Rosmarinus officinalis and Heracleum persicum essential oils against pathogenic Malassezia isolates. METHODS: The samples were collected from 5 different anatomical sites of 33 atopic dogs and cultured onto modified Dixon agar (MDA) and Sabouraud dextrose agar (SDA) media. The essential oil extraction was performed by steam distillation using Clevenger system. Anti-Malassezia efficacy of medicinal essential oils and standard drugs was evaluated using broth microdilution method. RESULTS: A total of 103 yeast colonies were isolated from dogs with AD. Eight different Malassezia species were identified as follows: Malassezia pachydermatis (81.4%), M. globosa (7.8%), M. restricta (3.9%), M. sloofiae (2.9%), M. furfur (1%), M. nana (1%), M. obtusa (1%) and M. sympodialis (1%). The most and least infected sites were: anal (21.2%) and ear (10.6%) respectively. M. pachydermatis was the most frequent Malassezia species isolated from both skin and mucosa of dogs with AD. Antifungal susceptibility test revealed the inhibitory efficacy of essential oils on pathogenic Malassezia isolates with minimum inhibitory concentration (MIC(90)) values ranging from 30 to 850 μg/mL. Among the tested oils, Z. multiflora and T. kotschyanus exhibited the highest inhibitory effects (P<0.05). CONCLUSION: The essential oils of Z. multiflora and T. kotschyanus showed strong antifungal activity against pathogenic Malassezia species tested. SN - 1773-0449 UR - https://www.unboundmedicine.com/medline/citation/26597143/Efficacy_of_medicinal_essential_oils_against_pathogenic_Malassezia_sp__isolates_ DB - PRIME DP - Unbound Medicine ER -